Skip to content

Research at St Andrews

Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system

Research output: Contribution to journalArticle

Author(s)

Li-Yang Hsu, Simon R Harris, Monika A Chlebowicz, Jodi A Lindsay, Tse-Hsien Koh, Prabha Krishnan, Thean-Yen Tan, Pei-Yun Hon, Warren B Grubb, Stephen D Bentley, Julian Parkhill, Sharon J Peacock, Matthew T G Holden

School/Research organisations

Abstract

Background

In the past decade, several countries have seen gradual replacement of endemic multi-resistant healthcare-associated methicillin-resistant Staphylococcus aureus (MRSA) with clones that are more susceptible to antibiotic treatment. One example is Singapore, where MRSA ST239, the dominant clone since molecular profiling of MRSA began in the mid-1980s, has been replaced by ST22 isolates belonging to EMRSA-15, a recently emerged pandemic lineage originating from Europe.

Results

We investigated the population structure of MRSA in Singaporean hospitals spanning three decades, using whole genome sequencing. Applying Bayesian phylogenetic methods we report that prior to the introduction of ST22, the ST239 MRSA population in Singapore originated from multiple introductions from the surrounding region; it was frequently transferred within the healthcare system resulting in a heterogeneous hospital population. Following the introduction of ST22 around the beginning of the millennium, this clone spread rapidly through Singaporean hospitals, supplanting the endemic ST239 population. Coalescent analysis revealed that although the genetic diversity of ST239 initially decreased as ST22 became more dominant, from 2007 onwards the genetic diversity of ST239 began to increase once more, which was not associated with the emergence of a sub-clone of ST239. Comparative genomic analysis of the accessory genome of the extant ST239 population identified that the Arginine Catabolic Mobile Element arose multiple times, thereby introducing genes associated with enhanced skin colonization into this population.

Conclusions

Our results clearly demonstrate that, alongside clinical practice and antibiotic usage, competition between clones also has an important role in driving the evolution of nosocomial pathogen populations.

Close

Details

Original languageEnglish
Article number81
Number of pages13
JournalGenome Biology
Volume16
DOIs
Publication statusPublished - 23 Apr 2015

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Draft genome sequences of the type strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), two NAD-dependent bacterial species found in the respiratory tract of pigs

    Bossé, J. T., Li, Y., Fernandez Crespo, R., Angen, Ø., Holden, M. T. G., Weinert, L. A., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R. & BRaDP1T Consortium, 2 Jan 2020, In : Microbiology Resource Announcements. 9, 3 p., e00716-19.

    Research output: Contribution to journalArticle

  2. The emergence of successful Streptococcus pyogenes lineages through convergent pathways of capsule loss and recombination directing high toxin expression

    Turner, C. E., Holden, M. T. G., Blane, B., Horner, C., Peacock, S. J. & Sriskandan, S., 10 Dec 2019, In : mBio. 10, 6, p. 1-19 20 p., e02521.

    Research output: Contribution to journalArticle

  3. Evolution and global transmission of a multidrug-resistant, community-associated methicillin-resistant Staphylococcus aureus lineage from the Indian subcontinent

    Steinig, E. J., Duchene, S., Robinson, D. A., Monecke, S., Yokoyama, M., Laabei, M., Slickers, P., Andersson, P., Williamson, D., Kearns, A., Goering, R. V., Dickson, E., Ehricht, R., Ip, M., O'Sullivan, M. V. N., Coombs, G. W., Petersen, A., Brennan, G., Shore, A. C., Coleman, D. C. & 18 others, Pantosti, A., de Lencastre, H., Westh, H., Kobayashi, N., Heffernan, H., Strommenger, B., Layer, F., Weber, S., Aamot, H. V., Skakni, L., Peacock, S. J., Sarovich, D., Harris, S., Parkhill, J., Massey, R. C., Holden, M. T. G., Bentley, S. D. & Tong, S. Y. C., 26 Nov 2019, In : mBio. 10, 6, p. 1-20 20 p., e01105.

    Research output: Contribution to journalArticle

  4. Genetic variation associated with infection and the environment in the accidental pathogen Burkholderia pseudomallei

    Chewapreecha, C., Mather, A. E., Harris, S. R., Hunt, M., Holden, M. T. G., Chaichana, C., Wuthiekanun, V., Dougan, G., Day, N. P. J., Limmathurotsakul, D., Parkhill, J. & Peacock, S. J., 22 Nov 2019, In : Communications Biology. 2, 11 p., 428.

    Research output: Contribution to journalArticle

  5. Improved characterisation of MRSA transmission using within-host bacterial sequence diversity

    Hall, M. D., Holden, M. TG., Srisomang, P., Mahavanakul, W., Wuthiekanun, V., Limmathurotsakul, D., Fountain, K., Parkhill, J., Nickerson, E. K., Peacock, S. J. & Fraser, C., 8 Oct 2019, In : eLife. 8, e46402.

    Research output: Contribution to journalArticle

Related by journal

  1. Wnt evolution and function shuffling in liberal and conservative chordate genomes

    Somorjai, I. M. L., Marti-Solans, J., Diaz-Gracia, M., Nishida, H., Imai, K., Escriva, H., Cañestro, C. & Albalat, R., 25 Jul 2018, In : Genome Biology. 19, 17 p., 98.

    Research output: Contribution to journalArticle

  2. Methicillin-resistant Staphylococcus aureus emerged long before the introduction of methicillin into clinical practice

    Harkins, C. P., Pichon, B., Doumith, M., Parkhill, J., Westh, H., Tomasz, A., de Lencastre, H., Bentley, S. D., Kearns, A. M. & Holden, M. T. G., 20 Jul 2017, In : Genome Biology. 18, 11 p., 130.

    Research output: Contribution to journalArticle

  3. The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence

    Eves-van den Akker, S., Laetsch, D. R., Thorpe, P., Lilley, C. J., Danchin, E. G. J., Da Rocha, M., Rancurel, C., Holroyd, N. E., Cotton, J. A., Szitenberg, A., Grenier, E., Montarry, J., Mimee, B., Duceppe, M-O., Boyes, I., Marvin, J. M. C., Jones, L. M., Yusup, H. B., Lafond-Lapalme, J., Esquibet, M. & 14 others, Sabeh, M., Rott, M., Overmars, H., Finkers-Tomczak, A., Smant, G., Koutsovoulos, G., Blok, V., Mantelin, S., Cock, P. J. A., Phillips, W., Henrissat, B., Urwin, P. E., Blaxter, M. & Jones, J. T., 10 Jun 2016, In : Genome Biology. 17, 1, p. 1-23 23 p., 124.

    Research output: Contribution to journalArticle

  4. 5-hydroxymethylcytosine marks promoters in colon that resist DNA hypermethylation in cancer

    Uribe-Lewis, S., Stark, R., Carroll, T., Dunning, M. J., Bachman, M., Ito, Y., Stojic, L., Halim, S., Vowler, S. L., Lynch, A. G., Delatte, B., de Bony, E. J., Colin, L., Defrance, M., Krueger, F., Silva, A. L., ten Hoopen, R., Ibrahim, A. E. K., Fuks, F. & Murrell, A., 1 Apr 2015, In : Genome Biology. 16, 15 p., 69.

    Research output: Contribution to journalArticle

ID: 183840848

Top