Skip to content

Research at St Andrews

Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system

Research output: Contribution to journalArticle

Standard

Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system. / Hsu, Li-Yang; Harris, Simon R; Chlebowicz, Monika A; Lindsay, Jodi A; Koh, Tse-Hsien; Krishnan, Prabha; Tan, Thean-Yen; Hon, Pei-Yun; Grubb, Warren B; Bentley, Stephen D; Parkhill, Julian; Peacock, Sharon J; Holden, Matthew Tg.

In: Genome Biology, Vol. 16, 81, 23.04.2015.

Research output: Contribution to journalArticle

Harvard

Hsu, L-Y, Harris, SR, Chlebowicz, MA, Lindsay, JA, Koh, T-H, Krishnan, P, Tan, T-Y, Hon, P-Y, Grubb, WB, Bentley, SD, Parkhill, J, Peacock, SJ & Holden, MT 2015, 'Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system', Genome Biology, vol. 16, 81. https://doi.org/10.1186/s13059-015-0643-z

APA

Hsu, L-Y., Harris, S. R., Chlebowicz, M. A., Lindsay, J. A., Koh, T-H., Krishnan, P., ... Holden, M. T. (2015). Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system. Genome Biology, 16, [81]. https://doi.org/10.1186/s13059-015-0643-z

Vancouver

Hsu L-Y, Harris SR, Chlebowicz MA, Lindsay JA, Koh T-H, Krishnan P et al. Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system. Genome Biology. 2015 Apr 23;16. 81. https://doi.org/10.1186/s13059-015-0643-z

Author

Hsu, Li-Yang ; Harris, Simon R ; Chlebowicz, Monika A ; Lindsay, Jodi A ; Koh, Tse-Hsien ; Krishnan, Prabha ; Tan, Thean-Yen ; Hon, Pei-Yun ; Grubb, Warren B ; Bentley, Stephen D ; Parkhill, Julian ; Peacock, Sharon J ; Holden, Matthew Tg. / Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system. In: Genome Biology. 2015 ; Vol. 16.

Bibtex - Download

@article{148f0f533b9a41bc8248435da2a17862,
title = "Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system",
abstract = "BackgroundIn the past decade, several countries have seen gradual replacement of endemic multi-resistant healthcare-associated methicillin-resistant Staphylococcus aureus (MRSA) with clones that are more susceptible to antibiotic treatment. One example is Singapore, where MRSA ST239, the dominant clone since molecular profiling of MRSA began in the mid-1980s, has been replaced by ST22 isolates belonging to EMRSA-15, a recently emerged pandemic lineage originating from Europe.ResultsWe investigated the population structure of MRSA in Singaporean hospitals spanning three decades, using whole genome sequencing. Applying Bayesian phylogenetic methods we report that prior to the introduction of ST22, the ST239 MRSA population in Singapore originated from multiple introductions from the surrounding region; it was frequently transferred within the healthcare system resulting in a heterogeneous hospital population. Following the introduction of ST22 around the beginning of the millennium, this clone spread rapidly through Singaporean hospitals, supplanting the endemic ST239 population. Coalescent analysis revealed that although the genetic diversity of ST239 initially decreased as ST22 became more dominant, from 2007 onwards the genetic diversity of ST239 began to increase once more, which was not associated with the emergence of a sub-clone of ST239. Comparative genomic analysis of the accessory genome of the extant ST239 population identified that the Arginine Catabolic Mobile Element arose multiple times, thereby introducing genes associated with enhanced skin colonization into this population.ConclusionsOur results clearly demonstrate that, alongside clinical practice and antibiotic usage, competition between clones also has an important role in driving the evolution of nosocomial pathogen populations.",
author = "Li-Yang Hsu and Harris, {Simon R} and Chlebowicz, {Monika A} and Lindsay, {Jodi A} and Tse-Hsien Koh and Prabha Krishnan and Thean-Yen Tan and Pei-Yun Hon and Grubb, {Warren B} and Bentley, {Stephen D} and Julian Parkhill and Peacock, {Sharon J} and Holden, {Matthew Tg}",
note = "SRH, SDB JP and MTGH were supported by Wellcome Trust grant 098051. LYH was funded by the National Medical Research Council Singapore. SP is funded by the UKCRC Translational Infection Research Initiative, and the NIHR Cambridge Biomedical Research Centre.",
year = "2015",
month = "4",
day = "23",
doi = "10.1186/s13059-015-0643-z",
language = "English",
volume = "16",
journal = "Genome Biology",
issn = "1465-6906",
publisher = "BioMed Central",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system

AU - Hsu, Li-Yang

AU - Harris, Simon R

AU - Chlebowicz, Monika A

AU - Lindsay, Jodi A

AU - Koh, Tse-Hsien

AU - Krishnan, Prabha

AU - Tan, Thean-Yen

AU - Hon, Pei-Yun

AU - Grubb, Warren B

AU - Bentley, Stephen D

AU - Parkhill, Julian

AU - Peacock, Sharon J

AU - Holden, Matthew Tg

N1 - SRH, SDB JP and MTGH were supported by Wellcome Trust grant 098051. LYH was funded by the National Medical Research Council Singapore. SP is funded by the UKCRC Translational Infection Research Initiative, and the NIHR Cambridge Biomedical Research Centre.

PY - 2015/4/23

Y1 - 2015/4/23

N2 - BackgroundIn the past decade, several countries have seen gradual replacement of endemic multi-resistant healthcare-associated methicillin-resistant Staphylococcus aureus (MRSA) with clones that are more susceptible to antibiotic treatment. One example is Singapore, where MRSA ST239, the dominant clone since molecular profiling of MRSA began in the mid-1980s, has been replaced by ST22 isolates belonging to EMRSA-15, a recently emerged pandemic lineage originating from Europe.ResultsWe investigated the population structure of MRSA in Singaporean hospitals spanning three decades, using whole genome sequencing. Applying Bayesian phylogenetic methods we report that prior to the introduction of ST22, the ST239 MRSA population in Singapore originated from multiple introductions from the surrounding region; it was frequently transferred within the healthcare system resulting in a heterogeneous hospital population. Following the introduction of ST22 around the beginning of the millennium, this clone spread rapidly through Singaporean hospitals, supplanting the endemic ST239 population. Coalescent analysis revealed that although the genetic diversity of ST239 initially decreased as ST22 became more dominant, from 2007 onwards the genetic diversity of ST239 began to increase once more, which was not associated with the emergence of a sub-clone of ST239. Comparative genomic analysis of the accessory genome of the extant ST239 population identified that the Arginine Catabolic Mobile Element arose multiple times, thereby introducing genes associated with enhanced skin colonization into this population.ConclusionsOur results clearly demonstrate that, alongside clinical practice and antibiotic usage, competition between clones also has an important role in driving the evolution of nosocomial pathogen populations.

AB - BackgroundIn the past decade, several countries have seen gradual replacement of endemic multi-resistant healthcare-associated methicillin-resistant Staphylococcus aureus (MRSA) with clones that are more susceptible to antibiotic treatment. One example is Singapore, where MRSA ST239, the dominant clone since molecular profiling of MRSA began in the mid-1980s, has been replaced by ST22 isolates belonging to EMRSA-15, a recently emerged pandemic lineage originating from Europe.ResultsWe investigated the population structure of MRSA in Singaporean hospitals spanning three decades, using whole genome sequencing. Applying Bayesian phylogenetic methods we report that prior to the introduction of ST22, the ST239 MRSA population in Singapore originated from multiple introductions from the surrounding region; it was frequently transferred within the healthcare system resulting in a heterogeneous hospital population. Following the introduction of ST22 around the beginning of the millennium, this clone spread rapidly through Singaporean hospitals, supplanting the endemic ST239 population. Coalescent analysis revealed that although the genetic diversity of ST239 initially decreased as ST22 became more dominant, from 2007 onwards the genetic diversity of ST239 began to increase once more, which was not associated with the emergence of a sub-clone of ST239. Comparative genomic analysis of the accessory genome of the extant ST239 population identified that the Arginine Catabolic Mobile Element arose multiple times, thereby introducing genes associated with enhanced skin colonization into this population.ConclusionsOur results clearly demonstrate that, alongside clinical practice and antibiotic usage, competition between clones also has an important role in driving the evolution of nosocomial pathogen populations.

UR - http://www.genomebiology.com/2015/16/1/81#sec9

U2 - 10.1186/s13059-015-0643-z

DO - 10.1186/s13059-015-0643-z

M3 - Article

VL - 16

JO - Genome Biology

JF - Genome Biology

SN - 1465-6906

M1 - 81

ER -

Related by author

  1. Genomic identification of cryptic susceptibility to penicillins and β-lactamase inhibitors in methicillin-resistant Staphylococcus aureus

    Harrison, E. M., Ba, X., Coll, F., Blane, B., Restif, O., Carvell, H., Köser, C. U., Jamrozy, D., Reuter, S., Lovering, A., Gleadall, N., Bellis, K. L., Uhlemann, A. C., Lowy, F. D., Massey, R. C., Grilo, I. R., Sobral, R., Larsen, J., Rhod Larsen, A., Vingsbo Lundberg, C. & 5 others, Parkhill, J., Paterson, G. K., Holden, M. T. G., Peacock, S. J. & Holmes, M. A., 24 Jun 2019, In : Nature Microbiology. 15 p.

    Research output: Contribution to journalArticle

  2. Atlas of group A streptococcal vaccine candidates compiled using large-scale comparative genomics

    Davies, M. R., McIntyre, L., Mutreja, A., Lacey, J. A., Lees, J. A., Towers, R. J., Duchêne, S., Smeesters, P. R., Frost, H. R., Price, D. J., Holden, M. T. G., David, S., Giffard, P. M., Worthing, K. A., Seale, A. C., Berkley, J. A., Harris, S. R., Rivera-Hernandez, T., Berking, O., Cork, A. J. & 18 others, Torres, R. S. L. A., Lithgow, T., Strugnell, R. A., Bergmann, R., Nitsche-Schmitz, P., Chhatwal, G. S., Bentley, S. D., Fraser, J. D., Moreland, N. J., Carapetis, J. R., Steer, A. C., Parkhill, J., Saul, A., Williamson, D. A., Currie, B. J., Tong, S. Y. C., Dougan, G. & Walker, M. J., Jun 2019, In : Nature Genetics. 51, 6, p. 1035-1043

    Research output: Contribution to journalArticle

  3. Antimicrobial resistance in atopic dermatitis: need for an urgent rethink

    Harkins, C. P., Holden, M. T. G. & Irvine, A. D., Mar 2019, In : Annals of Allergy Asthma & Immunology. 122, 3, p. 236-240 5 p.

    Research output: Contribution to journalArticle

  4. Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States

    Mohamed, N., Timofeyeva, Y., Jamrozy, D., Rojas, E., Hao, L., Silmon de Monerri, N. C., Hawkins, J., Singh, G., Cai, B., Liberator, P., Sebastian, S., Donald, R. G. K., Scully, I. L., Jones, C. H., Creech, C. B., Thomsen, I., Parkhill, J., Peacock, S. J., Jansen, K. U., Holden, M. T. G. & 1 others, Anderson, A. S., 14 Jan 2019, In : PLoS ONE. 14, 1, 33 p., e0208356.

    Research output: Contribution to journalArticle

Related by journal

  1. Wnt evolution and function shuffling in liberal and conservative chordate genomes

    Somorjai, I. M. L., Marti-Solans, J., Diaz-Gracia, M., Nishida, H., Imai, K., Escriva, H., Cañestro, C. & Albalat, R., 25 Jul 2018, In : Genome Biology. 19, 17 p., 98.

    Research output: Contribution to journalArticle

  2. Methicillin-resistant Staphylococcus aureus emerged long before the introduction of methicillin into clinical practice

    Harkins, C. P., Pichon, B., Doumith, M., Parkhill, J., Westh, H., Tomasz, A., de Lencastre, H., Bentley, S. D., Kearns, A. M. & Holden, M. T. G., 20 Jul 2017, In : Genome Biology. 18, 11 p., 130.

    Research output: Contribution to journalArticle

  3. The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence

    Eves-van den Akker, S., Laetsch, D. R., Thorpe, P., Lilley, C. J., Danchin, E. G. J., Da Rocha, M., Rancurel, C., Holroyd, N. E., Cotton, J. A., Szitenberg, A., Grenier, E., Montarry, J., Mimee, B., Duceppe, M-O., Boyes, I., Marvin, J. M. C., Jones, L. M., Yusup, H. B., Lafond-Lapalme, J., Esquibet, M. & 14 others, Sabeh, M., Rott, M., Overmars, H., Finkers-Tomczak, A., Smant, G., Koutsovoulos, G., Blok, V., Mantelin, S., Cock, P. J. A., Phillips, W., Henrissat, B., Urwin, P. E., Blaxter, M. & Jones, J. T., 10 Jun 2016, In : Genome Biology. 17, 1, p. 1-23 23 p., 124.

    Research output: Contribution to journalArticle

  4. 5-hydroxymethylcytosine marks promoters in colon that resist DNA hypermethylation in cancer

    Uribe-Lewis, S., Stark, R., Carroll, T., Dunning, M. J., Bachman, M., Ito, Y., Stojic, L., Halim, S., Vowler, S. L., Lynch, A. G., Delatte, B., de Bony, E. J., Colin, L., Defrance, M., Krueger, F., Silva, A. L., ten Hoopen, R., Ibrahim, A. E. K., Fuks, F. & Murrell, A., 1 Apr 2015, In : Genome Biology. 16, 15 p., 69.

    Research output: Contribution to journalArticle

ID: 183840848

Top