Skip to content

Research at St Andrews

Extensive Myzus cerasi transcriptional changes associated with detoxification genes upon primary to secondary host alternation

Research output: Contribution to journalArticle

Standard

Extensive Myzus cerasi transcriptional changes associated with detoxification genes upon primary to secondary host alternation. / Thorpe, Peter; Escudero-Martinez, Carmen; Eves-van den Akker, Sebastian; Bos, Jorunn.

In: biorxiv, 2018.

Research output: Contribution to journalArticle

Harvard

Thorpe, P, Escudero-Martinez, C, Eves-van den Akker, S & Bos, J 2018, 'Extensive Myzus cerasi transcriptional changes associated with detoxification genes upon primary to secondary host alternation', biorxiv. https://doi.org/10.1101/366450

APA

Thorpe, P., Escudero-Martinez, C., Eves-van den Akker, S., & Bos, J. (2018). Extensive Myzus cerasi transcriptional changes associated with detoxification genes upon primary to secondary host alternation. biorxiv. https://doi.org/10.1101/366450

Vancouver

Thorpe P, Escudero-Martinez C, Eves-van den Akker S, Bos J. Extensive Myzus cerasi transcriptional changes associated with detoxification genes upon primary to secondary host alternation. biorxiv. 2018. https://doi.org/10.1101/366450

Author

Thorpe, Peter ; Escudero-Martinez, Carmen ; Eves-van den Akker, Sebastian ; Bos, Jorunn. / Extensive Myzus cerasi transcriptional changes associated with detoxification genes upon primary to secondary host alternation. In: biorxiv. 2018.

Bibtex - Download

@article{894a550540a7431cb6294f181dd7b644,
title = "Extensive Myzus cerasi transcriptional changes associated with detoxification genes upon primary to secondary host alternation",
abstract = "Background: Aphids are phloem-feeding insects that cause yield losses to crops globally. These insects feature complex life cycles, which in the case of many agriculturally important species involves the use of primary and secondary host plant species. The switching between winter or primary hosts, on which overwintering eggs are laid upon sexual reproduction, and the secondary hosts, on which aphids reproduce asexually by parthenogenesis, is called host alternation. Here, we used Myzus cerasi (black cherry aphid), which overwinters on cherry trees and in summer spreads to herbaceous plant species, to assess aphid transcriptional changes that occur upon host alternation. Results: Adaptation experiments of M. cerasi collected from local cherry tress to reported secondary host species revealed low survival rates when aphids were moved to two secondary host species. Moreover, aphids were unable to survive on one of the reported hosts (Land cress) unless first adapted to another secondary host (cleavers). Transcriptome analyses of populations adapted to the primary host cherry and two secondary host species showed extensive transcriptional plasticity to host alternation, with predominantly genes involved in oxidation-reduction differentially regulated. Most of the differentially expressed genes across the M. cerasi populations from the different hosts were duplicated and we found evidence for differential exon usage. In contrast, we observed only limited transcriptional to secondary host switching. Conclusion: Aphid host alternation between summer and winter host plant species is an intriguing feature of aphid life cycles that is not well understood, especially at the molecular level. Here we show that, under controlled conditions, M. cerasi adaptation from primary to secondary host species does not readily occur and involves extensive changes in aphid gene expression. Our data suggests that different sets of genes involved in detoxification are required to feed from primary versus secondary host species.",
author = "Peter Thorpe and Carmen Escudero-Martinez and {Eves-van den Akker}, Sebastian and Jorunn Bos",
year = "2018",
doi = "10.1101/366450",
language = "Undefined/Unknown",
journal = "biorxiv",
publisher = "Cold Spring Harbor Laboratory Press",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Extensive Myzus cerasi transcriptional changes associated with detoxification genes upon primary to secondary host alternation

AU - Thorpe, Peter

AU - Escudero-Martinez, Carmen

AU - Eves-van den Akker, Sebastian

AU - Bos, Jorunn

PY - 2018

Y1 - 2018

N2 - Background: Aphids are phloem-feeding insects that cause yield losses to crops globally. These insects feature complex life cycles, which in the case of many agriculturally important species involves the use of primary and secondary host plant species. The switching between winter or primary hosts, on which overwintering eggs are laid upon sexual reproduction, and the secondary hosts, on which aphids reproduce asexually by parthenogenesis, is called host alternation. Here, we used Myzus cerasi (black cherry aphid), which overwinters on cherry trees and in summer spreads to herbaceous plant species, to assess aphid transcriptional changes that occur upon host alternation. Results: Adaptation experiments of M. cerasi collected from local cherry tress to reported secondary host species revealed low survival rates when aphids were moved to two secondary host species. Moreover, aphids were unable to survive on one of the reported hosts (Land cress) unless first adapted to another secondary host (cleavers). Transcriptome analyses of populations adapted to the primary host cherry and two secondary host species showed extensive transcriptional plasticity to host alternation, with predominantly genes involved in oxidation-reduction differentially regulated. Most of the differentially expressed genes across the M. cerasi populations from the different hosts were duplicated and we found evidence for differential exon usage. In contrast, we observed only limited transcriptional to secondary host switching. Conclusion: Aphid host alternation between summer and winter host plant species is an intriguing feature of aphid life cycles that is not well understood, especially at the molecular level. Here we show that, under controlled conditions, M. cerasi adaptation from primary to secondary host species does not readily occur and involves extensive changes in aphid gene expression. Our data suggests that different sets of genes involved in detoxification are required to feed from primary versus secondary host species.

AB - Background: Aphids are phloem-feeding insects that cause yield losses to crops globally. These insects feature complex life cycles, which in the case of many agriculturally important species involves the use of primary and secondary host plant species. The switching between winter or primary hosts, on which overwintering eggs are laid upon sexual reproduction, and the secondary hosts, on which aphids reproduce asexually by parthenogenesis, is called host alternation. Here, we used Myzus cerasi (black cherry aphid), which overwinters on cherry trees and in summer spreads to herbaceous plant species, to assess aphid transcriptional changes that occur upon host alternation. Results: Adaptation experiments of M. cerasi collected from local cherry tress to reported secondary host species revealed low survival rates when aphids were moved to two secondary host species. Moreover, aphids were unable to survive on one of the reported hosts (Land cress) unless first adapted to another secondary host (cleavers). Transcriptome analyses of populations adapted to the primary host cherry and two secondary host species showed extensive transcriptional plasticity to host alternation, with predominantly genes involved in oxidation-reduction differentially regulated. Most of the differentially expressed genes across the M. cerasi populations from the different hosts were duplicated and we found evidence for differential exon usage. In contrast, we observed only limited transcriptional to secondary host switching. Conclusion: Aphid host alternation between summer and winter host plant species is an intriguing feature of aphid life cycles that is not well understood, especially at the molecular level. Here we show that, under controlled conditions, M. cerasi adaptation from primary to secondary host species does not readily occur and involves extensive changes in aphid gene expression. Our data suggests that different sets of genes involved in detoxification are required to feed from primary versus secondary host species.

U2 - 10.1101/366450

DO - 10.1101/366450

M3 - Article

JO - biorxiv

JF - biorxiv

ER -

Related by author

  1. Metabarcoding reveals a high diversity of woody host-associated Phytophthora spp. in soils at public gardens and amenity woodlands in Britain

    Riddell, C. E., Frederickson-Matika, D., Armstrong, A. C., Elliot, M., Forster, J., Hedley, P. E., Morris, J., Thorpe, P., Cooke, D. EL., Pritchard, L., Sharp, P. M. & Green, S., 16 May 2019, In : PeerJ. 7, e6931.

    Research output: Contribution to journalArticle

  2. Shared transcriptional control and disparate gain and loss of aphid parasitism genes

    Thorpe, P., Escudero-Martinez, C. M., Cock, P. J. A., Eves-van den Akker, S. & Bos, J. I. B., 1 Oct 2018, In : Genome Biology and Evolution. 10, 10, p. 2716-2733

    Research output: Contribution to journalArticle

  3. The Phytophthora infestans haustorium is a site for secretion of diverse classes of infection-associated proteins

    Wang, S., Welsh, L., Thorpe, P., Whisson, S. C., Boevink, P. C. & Birch, P. R. J., 28 Aug 2018, In : mBio. 9, 4, 14 p., e01216-18.

    Research output: Contribution to journalArticle

  4. The transcriptomes of Xiphinema index and Longidorus elongatus suggest independent acquisition of some plant parasitism genes by horizontal gene transfer in early-branching nematodes

    Danchin, E. G. J., Perfus-Barbeoch, L., Rancurel, C., Thorpe, P., Da Rocha, M., Bajew, S., Neilson, R., Guzeeva, E. S., Da Silva, C., Guy, J., Labadie, K., Esmenjaud, D., Helder, J., Jones, J. T. & den Akker, S. E., 23 Oct 2017, In : Genes. 8, 10, 287.

    Research output: Contribution to journalArticle

Related by journal

  1. Plasma non-esterified fatty acids contribute to increased coagulability in type-2 diabetes through altered plasma zinc speciation

    Sobczak, A. I. S., Katundu, K. G. H., Phoenix, F. A., Khazaipoul, S., Yu, R., Lampiao, F., Stefanowicz, F., Blindauer, C. A., Pitt, S. J., Smith, T. K., Ajjan, R. A. & Stewart, A. J., 28 Aug 2019, In : biorxiv. 38 p.

    Research output: Contribution to journalArticle

  2. The ATP-dependent chromatin remodelling enzyme Uls1 prevents Topoisomerase II poisoning

    Swanston, A., Zabrady, K. & Ferreira, H. C., 2018, In : biorxiv.

    Research output: Contribution to journalArticle

ID: 256116226

Top