Skip to content

Research at St Andrews

Femtosecond optical transfection of cells: viability and efficiency

Research output: Contribution to journalArticle

DOI

Abstract

Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 mu J/cm(2), have found an average transfection efficiency of 50 +/- 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique. (c) 2006 Optical Society of America

Close

Details

Original languageEnglish
Pages (from-to)7125-7133
Number of pages9
JournalOptics Express
Volume14
Issue number16
DOIs
Publication statusPublished - 7 Aug 2006

    Research areas

  • INFRARED LASER-PULSES, GENE DELIVERY, SINGLE CELLS, OPTOPORATION

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Optical transfection of mammalian cells - art. no. 61910D

    Stevenson, D. J., Agate, B., Paterson, L., Lake, T., Comrie, M., Brown, T., Riches, A., Bryant, P., Sibbett, W., Gunn-Moore, F. & Dholakia, K., 2006, Biophotonics and new therapy frontiers . Grzymala, R. & Haeberle, O. (eds.). SPIE, p. 1910-1910 8 p. (Proceeding of SPIE - The International Society for Optical Engineering; vol. 6191).

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

  2. Photoporation and cell transfection using a violet diode laser

    Paterson, L., Agate, B., Comrie, M., Ferguson, R., Lake, TK., Morris, JE., Carruthers, AE., Brown, C. T. A., Sibbett, W., Bryant, P. E., Gunn-Moore, F. J., Riches, A. C. & Dholakia, K., 24 Jan 2005, In : Optics Express. 13, 2, p. 595-600 6 p.

    Research output: Contribution to journalArticle

  3. Enhanced operation of femtosecond lasers and applications in cell transfection

    Brown, C. T. A., Stevenson, DJ., Tsampoula, X., McDougall, C., Lagatsky, AA., Sibbett, W., Gunn-Moore, F. J. & Dholakia, K., Aug 2008, In : Journal of Biophotonics. 1, 3, p. 183-199 17 p.

    Research output: Contribution to journalArticle

  4. Light-induced cell separation in a tailored optical landscape

    Paterson, L., Papagiakoumou, E., Milne, G. F., Garces-Chavez, V., Tatarkova, S. A., Sibbett, W., Gunn-Moore, F. J., Bryant, P. E., Riches, A. C. & Dholakia, K., 19 Sep 2005, In : Applied Physics Letters. 87, 12, p. 123901 3 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Broadly tunable femtosecond pulses around 2.06 µm from a diode-pumped Tm3+-doped solid-state laser source

    Stevenson, N. K., Brown, C. T. A., Hopkins, J-M., Dawson, M. D. & Lagatsky, A. A., 15 Apr 2019, In : Optics Express. 27, 8, p. 11103-11111

    Research output: Contribution to journalArticle

  2. Frequency modulated hybrid photonic crystal laser by thermal tuning

    Butler, S. M., Bakoz, A. P., Singaravelu, P. K. J., Liles, A. A., O’Shaughnessy, B., Viktorov, E. A., O’Faolain, L. & Hegarty, S. P., 15 Apr 2019, In : Optics Express. 27, 8, p. 11312-11322 11 p.

    Research output: Contribution to journalArticle

  3. Label-free optical hemogram of granulocytes enhanced by artificial neural networks

    Gupta, R., Chen, M., Malcolm, G. P. A., Hempler, N., Dholakia, K. & Powis, S. J., 13 May 2019, In : Optics Express. 27, 10, p. 13706-13720 15 p.

    Research output: Contribution to journalArticle

  4. Resolving the temporal evolution of line broadening in single quantum emitters

    Schimpf, C., Reindl, M., Klenovský, P., Fromherz, T., Covre Da Silva, S. F., Hofer, J., Schneider, C., Höfling, S., Trotta, R. & Rastelli, A., 18 Nov 2019, In : Optics Express. 27, 24, p. 35290-35307 18 p.

    Research output: Contribution to journalArticle

  5. Stochastic polarization switching induced by optical injection in bimodal quantum-dot micropillar lasers

    Schlottmann, E., Schicke, D., Krüger, F., Lingnau, B., Schneider, C., Höfling, S., Lüdge, K., Porte, X. & Reitzenstein, S., 24 Sep 2019, In : Optics Express. 27, 20, p. 28816-28831

    Research output: Contribution to journalArticle

Related by journal

  1. Optics Express (Journal)

    Graham David Bruce (Reviewer)
    2017 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. Optics Express (Journal)

    Michael Peter MacDonald (Editor)
    2005 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  3. Optics Express (Journal)

    Friedrich Ernst Wilhelm Koenig (Editor)
    2005 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  4. Optics Express (Journal)

    C Tom A Brown (Editor)
    2004 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

ID: 334178

Top