Research output: Contribution to journal › Article › peer-review
D Stevenson, B Agate, X Tsampoula, P Fischer, Christian Thomas Alcuin Brown, Wilson Sibbett, Andrew Clive Riches, Francis James Gunn-Moore, Kishan Dholakia
Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 mu J/cm(2), have found an average transfection efficiency of 50 +/- 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique. (c) 2006 Optical Society of America
Original language | English |
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Pages (from-to) | 7125-7133 |
Number of pages | 9 |
Journal | Optics Express |
Volume | 14 |
Issue number | 16 |
DOIs | |
Publication status | Published - 7 Aug 2006 |
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Graham David Bruce (Reviewer)
Activity: Publication peer-review and editorial work types › Peer review of manuscripts
Michael Peter MacDonald (Editor)
Activity: Publication peer-review and editorial work types › Editor of research journal
Friedrich Ernst Wilhelm Koenig (Editor)
Activity: Publication peer-review and editorial work types › Editor of research journal
C Tom A Brown (Editor)
Activity: Publication peer-review and editorial work types › Editor of research journal
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