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Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis

Research output: Contribution to journalArticle

Author(s)

Bavishna Balagopal, Praveen Cheriyan Ashok, Michael Mazilu, Andrew Clive Riches, C Simon Herrington, Kishan Dholakia

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Abstract

In the field of biomedical optics, Raman spectroscopy is a powerful tool for probing the chemical composition of biological samples. In particular, fiber Raman probes play a crucial role for in vivo and ex vivo tissue analysis. However, the high-fluorescence background typically contributed by the auto fluorescence from both a tissue sample and the fiber-probe interferes strongly with the relatively weak Raman signal. Here we demonstrate the implementation of wavelength-modulated Raman spectroscopy (WMRS) to suppress the fluorescence background while analyzing tissues using fiber Raman probes. We have observed a significant signal-to-noise ratio enhancement in the Raman bands of bone tissue, which have a relatively high fluorescence background. Implementation of WMRS in fiber-probe-based bone tissue study yielded usable Raman spectra in a relatively short acquisition time (∼30  s), notably without any special sample preparation stage. Finally, we have validated its capability to suppress fluorescence on other tissue samples such as adipose tissue derived from four different species.
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Details

Original languageEnglish
Article number077006
Number of pages6
JournalJournal of Biomedical Optics
Volume17
Issue number7
DOIs
Publication statusPublished - 9 Jul 2012

    Research areas

  • Biophotonics, Bone tissue studies, Raman probe, Raman spectroscopy, Tissues

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