Skip to content

Research at St Andrews

Gene amplification of atypical PKC-binding PARD3 in raditation-transformed neoplastic retinal pigment epithelial cell lines

Research output: Contribution to journalArticle

DOI

Author(s)

H Zitzelsberger, L Hieber, H Richter, K Unger, CV Briscoe, Clare Peddie, Andrew Clive Riches

School/Research organisations

Abstract

Neoplastic transformation induced by ionizing radiation was studied using a human retinal pigment epithelial cell line immortalized by telomerase. Radiation-transformed cell clones were tumorigenic in athymic mice and were analyzed by G-banding and comparative genomic hybridization (CGH). Radiation-transformed cloned cell lines and cell lines derived from tumors produced in athymic nude mice following transplantation exhibited a recurrent karyotype:45,XX,der(10),-13. CGH showed an amplification of 10p11.2 and a deletion of the remaining 10p. Positional cloning of the amplified region by FISH analysis and subsequent sequence analysis of BAC clones showing amplified FISH signals identified the candidate gene PARD3. This gene also was found to be transcriptionally expressed at an increased level. The findings indicate that PARD3 may play an important role in radiation-induced carcinogenesis of RPE cells. This is the first evidence for PARD3 amplification in human cancer cells. (C) 2004 Wiley-Liss, Inc.

Close

Details

Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalGenes, Chromosomes and Cancer
Volume40
Issue number1
DOIs
Publication statusPublished - May 2004

    Research areas

  • IN-VITRO, GAMMA-IRRADIATION, ALPHA-PARTICLES, LIFE-SPAN, TELOMERASE, EXPRESSION, EXPOSURE, POLARITY, COMPLEX, SINGLE

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Clonal chromosomal abberations in simian virus 40-transfected human thyroid cells and in derived tumours developed after in vitro irradiation.

    Zitzelsberger, H., Bruch, J., Smida, J., Hieber, L., Peddie, C., Bryant, P. E., Riches, A. C., Jingly, F., Heinz-Ulrich, G. & Bauchinger, M., 20 Jun 2001, In : International Journal of Cancer. 96, p. 166-177 12 p.

    Research output: Contribution to journalArticle

  2. Chromosomal aberrations in simian virus 40 transfected humnan thyroid cells and in derived tumors developed after in vitro irradiation

    Bruch, J., Zitzelsberger, H., Smida, J., Hieber, L., Peddie, C., Bryant, P. E., Riches, A. C., Fung, J., Weier, H-UG. & Bauchinger, M., 20 Jun 2001

    Research output: Other contribution

  3. Neoplastic Transformation and Cytogenetic Changes after Gamma Irradiation of Human Epithelial Cells Expressing Telomerase

    Riches, A. C., Peddie, C., Rendell, S., Bryant, P. E., Zitzelsberger, H., Bruch, J., Smida, J., Hieber, L. & Bauchinger, M., Jan 2001, In : Radiation Research. 155, 1, p. 222-229 8 p.

    Research output: Contribution to journalArticle

  4. Radiation-induced transformation of human thyroid epithelial cells

    Riches, A. C., Bryant, P. E., Peddie, C., Zitzelsberger, H., Smida, J., Bruch, J., Hieber, L., Weier, H-U. & Moriaty, M. D., 2000, p. 553-559.

    Research output: Contribution to conferencePaper

Related by journal

  1. Differential TERT promoter methylation and response to 5-aza-2'-deoxycytidine in acute myeloid leukemia cell lines: TERT expression, telomerase activity, telomere length, and cell death.

    Pettigrew, K. A., Armstrong, R., Colyer, H., Zhang, S-D., Rea, I. M., Jones, R., Baird, D. & Mills, K., Aug 2012, In : Genes, Chromosomes and Cancer. 51, 8, p. 768-780 13 p., 51(8).

    Research output: Contribution to journalArticle

  2. Localization of a novel t(1;7) translocation associated with Wilms' tumor predisposition and skeletal abnormalities

    Reynolds, P. A., Powlesland, R., Keen, TJ., Inglehearn, C., Cunningham, AE., Green, ED. & Brown, KW., Nov 1996, In : Genes, Chromosomes and Cancer. 17, 3, p. 151-155 5 p.

    Research output: Contribution to journalArticle

ID: 305300

Top