Skip to content

Research at St Andrews

University > Research portal > Research publications > Haem-iron plays a key role in the regulation of the Ess/type...

Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390

Research output: Contribution to journal › Article

DOI

Open Access permissions

Open

Links

Author(s)

M Guillermina Casabona, Holger Kneuper, Daniela Alferes de Lima, Catriona P Harkins, Martin Zoltner, Erik Hjerde, Matthew T. G. Holden, Tracy Palmer

School/Research organisations

Abstract

The Staphylococcus aureus type VII protein secretion system (T7SS) plays important roles in virulence and intra-species competition. Here we show that the T7SS in strain RN6390 is activated by supplementing the growth medium with haemoglobin, and its cofactor haemin (haem B). Transcript analysis and secretion assays suggest that activation by haemin occurs at a transcriptional and a post-translational level. Loss of T7 secretion activity by deletion of essC results in upregulation of genes required for iron acquisition. Taken together these findings suggest that the T7SS plays a role in iron homeostasis in at least some S. aureus strains.

Close
More...

Details

Original languageEnglish
Pages (from-to)1839-1850
JournalMicrobiology
Volume163
Issue number12
Early online date24 Nov 2017
DOIs
StatePublished - 1 Dec 2017

    Research areas

  • Staphylococcus aureus, Protein secretion, Iron homeostasis, RNA-sequencing

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The widespread use of topical antimicrobials enriches for resistance in Staphylococcus aureus isolated from Atopic Dermatitis patients

    Harkins, C. P., McAleer, M. A., Bennett, D., McHugh, M., Fleury, O. M., Pettigrew, K. A., Oravcová, K., Parkhill, J., Proby, C. M., Dawe, R. S., Geoghegan, J. A., Irvine, A. D. & Holden, M. T. G. 24 Jul 2018 In : British Journal of Dermatology. Early View, 8 p.

    Research output: Contribution to journal › Article

  2. Gene exchange drives the ecological success of a multi-host bacterial pathogen

    Richardson, E. J., Bacigalupe, R., Harrison, E. M., Weinert, L. A., Lycett, S., Vrieling, M., Robb, K., Hoskisson, P. A., Holden, M. T. G., Feil, E. J., Paterson, G. K., Tong, S. Y. C., Shittu, A., van Wamel, W., Aanensen, D. M., Parkhill, J., Peacock, S. J., Corander, J., Holmes, M. & Fitzgerald, J. R. 23 Jul 2018 In : Nature Ecology and Evolution. 2, p. 1468-1478

    Research output: Contribution to journal › Article

  3. Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility

    Krishna, A., Holden, M. T. G., Peacock, S. J., Edwards, A. M. & Wigneshweraraj, S. 20 Jul 2018 In : Microbiology. Latest Article

    Research output: Contribution to journal › Article

  4. Global scale dissemination of ST93: a divergent Staphylococcus aureus epidemic lineage that has recently emerged from remote Northern Australia

    van Hal, S. J., Steinig, E. J., Andersson, P., Holden, M. T. G., Harris, S. R., Nimmo, G. R., Williamson, D. A., Heffernan, H., Ritchie, S. R., Kearns, A. M., Ellington, M. J., Dickson, E., de Lencastre, H., Coombs, G. W., Bentley, S. D., Parkhill, J., Holt, D. C., Giffard, P. M. & Tong, S. Y. C. 9 Jul 2018 In : Frontiers in Microbiology. 9, 11 p., 1453

    Research output: Contribution to journal › Article

  5. Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing

    Bossé, J. T., Li, Y., Fernandez Crespo, R., Lacouture, S., Gottschalk, M., Sárközi, R., Fodor, L., Casas Amoribieta, M., Angen, Ø., Nedbalcova, K., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R. & BRaDP1T Consortium Jul 2018 In : Veterinary Microbiology. 220, p. 83-89 7 p.

    Research output: Contribution to journal › Article

Related by journal

  1. Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility

    Krishna, A., Holden, M. T. G., Peacock, S. J., Edwards, A. M. & Wigneshweraraj, S. 20 Jul 2018 In : Microbiology. Latest Article

    Research output: Contribution to journal › Article

  2. Functional analysis of the EsaB component of the Staphylococcus aureus Type VII secretion system

    Casabona, M. G., Buchanan, G., Zoltner, M., Harkins, C. P., Holden, M. T. G. & Palmer, T. 1 Dec 2017 In : Microbiology. 163, 12, p. 1851-1863 13 p.

    Research output: Contribution to journal › Article

  3. High-affinity nitrate/nitrite transporters NrtA and NrtB of Aspergillus nidulans exhibit high specificity and different inhibitor sensitivity

    Akhtar, N., Karabika, E., Kinghorn, J. R., Glass, A. D. M., Unkles, S. E. & Rouch, D. A. 1 Jul 2015 In : Microbiology. 161, 7, p. 1435-1446

    Research output: Contribution to journal › Article

  4. PinR mediates the generation of reversible population diversity in Streptococcus zooepidemicus

    Steward, K. F., Harrison, T., Robinson, C., Slater, J., Maskell, D. J., Harris, S. R., Holden, M. T. G. & Waller, A. S. May 2015 In : Microbiology. 161, 5, p. 1105-1112

    Research output: Contribution to journal › Article

Related by journal

  1. Microbiology (Journal)

    Holden, M. (Editor)
    2013

    Activity: Publication peer-review and editorial work › Editor of research journal

ID: 251666266