Skip to content

Research at St Andrews

Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390

Research output: Contribution to journalArticle

DOI

Open Access permissions

Open

Author(s)

M Guillermina Casabona, Holger Kneuper, Daniela Alferes de Lima, Catriona P Harkins, Martin Zoltner, Erik Hjerde, Matthew T. G. Holden, Tracy Palmer

School/Research organisations

Abstract

The Staphylococcus aureus type VII protein secretion system (T7SS) plays important roles in virulence and intra-species competition. Here we show that the T7SS in strain RN6390 is activated by supplementing the growth medium with haemoglobin, and its cofactor haemin (haem B). Transcript analysis and secretion assays suggest that activation by haemin occurs at a transcriptional and a post-translational level. Loss of T7 secretion activity by deletion of essC results in upregulation of genes required for iron acquisition. Taken together these findings suggest that the T7SS plays a role in iron homeostasis in at least some S. aureus strains.

Close

Details

Original languageEnglish
Pages (from-to)1839-1850
JournalMicrobiology
Volume163
Issue number12
Early online date24 Nov 2017
DOIs
StatePublished - 1 Dec 2017

    Research areas

  • Staphylococcus aureus, Protein secretion, Iron homeostasis, RNA-sequencing

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Global scale dissemination of ST93: a divergent Staphylococcus aureus epidemic lineage that has recently emerged from remote Northern Australia

    van Hal, S. J., Steinig, E. J., Andersson, P., Holden, M. T. G., Harris, S. R., Nimmo, G. R., Williamson, D. A., Heffernan, H., Ritchie, S. R., Kearns, A. M., Ellington, M. J., Dickson, E., de Lencastre, H., Coombs, G. W., Bentley, S. D., Parkhill, J., Holt, D. C., Giffard, P. M. & Tong, S. Y. C. 9 Jul 2018 In : Frontiers in Microbiology. 9, 11 p., 1453

    Research output: Contribution to journalArticle

  2. Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing

    Bossé, J. T., Li, Y., Fernandez Crespo, R., Lacouture, S., Gottschalk, M., Sárközi, R., Fodor, L., Casas Amoribieta, M., Angen, Ø., Nedbalcova, K., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R. & on behalf of the BRaDP1T consortium Jul 2018 In : Veterinary Microbiology. 220, p. 83-89 7 p.

    Research output: Contribution to journalArticle

  3. An outbreak of Streptococcus pyogenes in a mental health facility: advantage of well-timed whole-genome sequencing over emm typing

    Bergin, S. M. , Periaswamy, B. , Barkham, T. , Chua, H. C. , Mok, Y. M. , Fung, D. S. S. , Su, A. H. C. , Lee, Y. L. , Chua, M. L. I. , Ng, P. Y. , Soon, W. J. W. , Chu, C. W. , Tan, S. L. , Meehan, M. , Ang, B. S. P. , Leo, Y. S. , Holden, M. T. G. , De, P. , Hsu, L. Y. , Chen, S. L. & 2 others de Sessions, P. F. & Marimuthu, K. 9 May 2018 In : Infection Control and Hospital Epidemiology. First View, 9 p.

    Research output: Contribution to journalArticle

  4. The widespread use of topical antimicrobials enriches for resistance in Staphylococcus aureus isolated from Atopic Dermatitis patients

    Harkins, C. P., McAleer, M. A., Bennett, D., McHugh, M., Fleury, O. M., Pettigrew, K. A., Oravcová, K., Parkhill, J., Proby, C. M., Dawe, R. S., Geoghegan, J. A., Irvine, A. D. & Holden, M. T. G. 5 May 2018 (Accepted/In press) In : British Journal of Dermatology. In press

    Research output: Contribution to journalArticle

  5. Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA

    Hao, L., Holden, M. T. G., Wang, X., Andrew, L., Wellnitz, S., Hu, F., Whaley, M., Sammons, S., Knipe, K., Frace, M., McNamara, L. A., Liberator, P. & Anderson, A. S. Apr 2018 In : Microbial Genomics. 4, 4, 10 p., e000155

    Research output: Contribution to journalArticle

Related by journal

  1. Functional analysis of the EsaB component of the Staphylococcus aureus Type VII secretion system

    Casabona, M. G., Buchanan, G., Zoltner, M., Harkins, C. P., Holden, M. T. G. & Palmer, T. 1 Dec 2017 In : Microbiology. 163, 12, p. 1851-1863 13 p.

    Research output: Contribution to journalArticle

  2. High-affinity nitrate/nitrite transporters NrtA and NrtB of Aspergillus nidulans exhibit high specificity and different inhibitor sensitivity

    Akhtar, N., Karabika, E., Kinghorn, J. R., Glass, A. D. M., Unkles, S. E. & Rouch, D. A. 1 Jul 2015 In : Microbiology. 161, 7, p. 1435-1446

    Research output: Contribution to journalArticle

  3. PinR mediates the generation of reversible population diversity in Streptococcus zooepidemicus

    Steward, K. F., Harrison, T., Robinson, C., Slater, J., Maskell, D. J., Harris, S. R., Holden, M. T. G. & Waller, A. S. May 2015 In : Microbiology. 161, 5, p. 1105-1112

    Research output: Contribution to journalArticle

Related by journal

  1. Microbiology (Journal)

    Holden, M. (Editor)
    2013

    Activity: Publication peer-review and editorial workEditor of research journal

ID: 251666266