Skip to content

Research at St Andrews

High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi

Research output: Contribution to journalArticle

Author(s)

Piengchan Sonthayanon, Sharon J. Peacock, Wirongrong Chierakul, Vanaporn Wuthiekanun, Stuart D. Blacksell, Mathew T. G. Holden, Stephen D. Bentley, Edward J. Feil, Nicholas P. J. Day, Matthew Holden

School/Research organisations

Abstract

Orientia tsutsugamushi is an intracellular alpha-proteobacterium which resides in trombiculid mites, and is the causative agent of scrub typhus in East Asia. The genome sequence of this species has revealed an unprecedented number of repeat sequences, most notably of the genes encoding the conjugative properties of a type IV secretion system (T4SS). Although this observation is consistent with frequent intragenomic recombination, the extent of homologous recombination (gene conversion) in this species is unknown. To address this question, and to provide a protocol for the epidemiological surveillance of this important pathogen, we have developed a multilocus sequence typing (MLST) scheme based on 7 housekeeping genes (gpsA, mdh, nrdB, nuoF, ppdK, sucD, sucB). We applied this scheme to the two published genomes, and to DNA extracted from blood taken from 84 Thai scrub typhus patients, from 20 cultured Thai patient isolates, 1 Australian patient sample, and from 3 cultured type strains. These data demonstrated that the O. tsutsugamushi population was both highly diverse [Simpson's index (95% CI) = 0.95 (0.92-0.98)], and highly recombinogenic. These results are surprising given the intracellular life-style of this species, but are broadly consistent with results obtained for Wolbachia, which is an alpha-proteobacterial reproductive parasite of arthropods. We also compared the MLST data with ompA sequence data and noted low levels of consistency and much higher discrimination by MLST. Finally, twenty-five percent of patients in this study were simultaneously infected with multiple sequence types, suggesting multiple infection caused by either multiple mite bites, or multiple strains co-existing within individual mites.

Close

Details

Original languageEnglish
Article number752
Number of pages10
JournalPLoS Neglected Tropical Diseases
Volume4
Issue number7
DOIs
Publication statusPublished - Jul 2010

    Research areas

  • Scrub typhus, Relative contributions, Typing data, Mutation, Microorganisms, Rickettsia, Divergence, Diversity, Thailand, Reveals

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Genomic identification of cryptic susceptibility to penicillins and β-lactamase inhibitors in methicillin-resistant Staphylococcus aureus

    Harrison, E. M., Ba, X., Coll, F., Blane, B., Restif, O., Carvell, H., Köser, C. U., Jamrozy, D., Reuter, S., Lovering, A., Gleadall, N., Bellis, K. L., Uhlemann, A. C., Lowy, F. D., Massey, R. C., Grilo, I. R., Sobral, R., Larsen, J., Rhod Larsen, A., Vingsbo Lundberg, C. & 5 othersParkhill, J., Paterson, G. K., Holden, M. T. G., Peacock, S. J. & Holmes, M. A., 24 Jun 2019, In : Nature Microbiology. 15 p.

    Research output: Contribution to journalArticle

  2. Atlas of group A streptococcal vaccine candidates compiled using large-scale comparative genomics

    Davies, M. R., McIntyre, L., Mutreja, A., Lacey, J. A., Lees, J. A., Towers, R. J., Duchêne, S., Smeesters, P. R., Frost, H. R., Price, D. J., Holden, M. T. G., David, S., Giffard, P. M., Worthing, K. A., Seale, A. C., Berkley, J. A., Harris, S. R., Rivera-Hernandez, T., Berking, O., Cork, A. J. & 18 othersTorres, R. S. L. A., Lithgow, T., Strugnell, R. A., Bergmann, R., Nitsche-Schmitz, P., Chhatwal, G. S., Bentley, S. D., Fraser, J. D., Moreland, N. J., Carapetis, J. R., Steer, A. C., Parkhill, J., Saul, A., Williamson, D. A., Currie, B. J., Tong, S. Y. C., Dougan, G. & Walker, M. J., Jun 2019, In : Nature Genetics. 51, 6, p. 1035-1043

    Research output: Contribution to journalArticle

  3. Antimicrobial resistance in atopic dermatitis: need for an urgent rethink

    Harkins, C. P., Holden, M. T. G. & Irvine, A. D., Mar 2019, In : Annals of Allergy Asthma & Immunology. 122, 3, p. 236-240 5 p.

    Research output: Contribution to journalArticle

  4. Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States

    Mohamed, N., Timofeyeva, Y., Jamrozy, D., Rojas, E., Hao, L., Silmon de Monerri, N. C., Hawkins, J., Singh, G., Cai, B., Liberator, P., Sebastian, S., Donald, R. G. K., Scully, I. L., Jones, C. H., Creech, C. B., Thomsen, I., Parkhill, J., Peacock, S. J., Jansen, K. U., Holden, M. T. G. & 1 othersAnderson, A. S., 14 Jan 2019, In : PLoS ONE. 14, 1, 33 p., e0208356.

    Research output: Contribution to journalArticle

Related by journal

  1. PLoS Neglected Tropical Diseases (Journal)

    Simon Young (Reviewer)
    9 Oct 2017 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. PLoS Neglected Tropical Diseases (Journal)

    Terry K Smith (Member of editorial board)
    2015 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  3. PLoS Neglected Tropical Diseases (Journal)

    Simon Young (Reviewer)
    9 Aug 201114 Dec 2011

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. Inhibitors of Trypanosoma cruzi Sir2 related protein 1 as potential drugs against Chagas disease

    Gaspar, L., Coron, R. P., Kongthoo Lin, P., Costa, D. M., Perez-Cabezas, B., Tavares, J., Roura-Ferrer, M., Ramos, I., Ronin, C., Major, L. L., Ciesielski, F., Pemberton, I. K., MacDougall, J., Ciapetti, P., Smith, T. K. & Cordeiro-da-Silva, A., 22 Jan 2018, In : PLoS Neglected Tropical Diseases. 12, 1, 30 p., e0006180.

    Research output: Contribution to journalArticle

  2. Hepatitis B virus infection as a neglected tropical disease

    O’Hara, G. A., McNaughton, A. L., Maponga, T., Jooste, P., Ocama, P., Chilengi, R., Mokaya, J., Liyayi, M. I., Wachira, T., Gikungi, D. M., Burbridge, L., O’Donnell, D., Akiror, C. S., Sloan, D., Torimiro, J., Yindom, L. M., Walton, R., Andersson, M., Marsh, K., Newton, R. & 1 othersMatthews, P. C., 5 Oct 2017, In : PLoS Neglected Tropical Diseases. 11, 10, e0005842.

    Research output: Contribution to journalArticle

  3. Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues

    Tulloch, L. B., Menzies, S. K., Fraser, A. L., Gould, E. R., King, E. F., Zacharova, M. K., Florence, G. J. & Smith, T. K., 5 Sep 2017, In : PLoS Neglected Tropical Diseases. 11, 9, 28 p., e0005886.

    Research output: Contribution to journalArticle

  4. Sterol 14α-demethylase mutation leads to amphotericin B resistance in Leishmania mexicana

    Mwenechanya, R., Kovářová, J., Dickens, N. J., Mudaliar, M., Herzyk, P., Vincent, I. M., Weidt, S. K., Burgess, K. E., Burchmore, R. J. S., Pountain, A. W., Smith, T. K., Creek, D. J., Kim, D-H., Lepesheva, G. I. & Barrett, M. P., 16 Jun 2017, In : PLoS Neglected Tropical Diseases. 11, 6, 21 p., e0005649.

    Research output: Contribution to journalArticle

  5. Different mutations in a P-type ATPase transporter in Leishmania parasites are associated with cross-resistance to two leading drugs by distinct mechanisms

    Fernandez-Prada, C., Vincent, I. M., Brotherton, M-C., Roberts, M., Roy, G., Rivas, L., Leprohon, P., Smith, T. K. & Ouellette, M., 2 Dec 2016, In : PLoS Neglected Tropical Diseases. 10, 12, 20 p., e0005171.

    Research output: Contribution to journalArticle

ID: 91764610