Skip to content

Research at St Andrews

HPV16 E7 oncoprotein deregulates B-myb expression: correlation with targeting of p107/E2F complexes

Research output: Contribution to journalArticlepeer-review

Author(s)

E W Lam, J D Morris, R Davies, T Crook, R J Watson, K H Vousden

School/Research organisations

Abstract

HPV16 is a human tumour virus encoding two principal oncoproteins, E6 and E7. Expression of E7 can induce DNA synthesis in quiescent cells and this property coincides with its ability to bind to the cell proteins pRb and p107. As these cell proteins are regulators of the transcription factor E2F, we have investigated whether the interaction with E7 could result in induction of cell cycle regulated genes. We show that B-myb, whose induction at the G1/S boundary is regulated by release from E2F mediated transcriptional repression, is a target for transcriptional activation by E7 and is the first E7 responsive cell gene to be identified. E7 transactivation leads to both inappropriate transcription of B-myb during G1 and constitutive over-expression in cycling cells. B-Myb plays an essential role in cell cycle progression, and activation by E7 is likely to contribute to the mitogenic activity of the viral oncoprotein. Regulation of the B-myb promoter in NIH3T3 cells correlates with binding of distinct p107-containing complexes at the E2F binding site, and analysis of E7 mutants confirms that B-myb transcription in these cells is regulated through interactions with p107 rather than pRb. These results provide the first example of a potentially specific role for p107 in the regulation of the cell cycle.

Close

Details

Original languageEnglish
Pages (from-to)871-8
Number of pages8
JournalEMBO Journal
Volume13
Issue number4
Publication statusPublished - 15 Feb 1994

    Research areas

  • 3T3 Cells, Animals, Base Sequence, Carrier Proteins, Cell Cycle Proteins, Cell Line, Transformed, Cyclins/metabolism, DNA-Binding Proteins/biosynthesis, E2F Transcription Factors, G1 Phase, Humans, Keratinocytes/metabolism, Mice, Molecular Sequence Data, Nuclear Proteins, Oligodeoxyribonucleotides, Oncogene Proteins, Viral/metabolism, Papillomavirus E7 Proteins, Promoter Regions, Genetic, Proteins/metabolism, Retinoblastoma-Binding Protein 1, Retinoblastoma-Like Protein p107, Trans-Activators, Transcription Factor DP1, Transcription Factors/biosynthesis, Transcriptional Activation

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Association of human papillomavirus 16 E2 with Rad50-interacting protein 1 enhances viral DNA replication

    Campos-León, K., Wijendra, K., Siddiqa, A., Pentland, I., Feeney, K. M., Knapman, A., Davies, R., Androphy, E. J. & Parish, J. L., Mar 2017, In: Journal of Virology. 91, 5, 18 p., e02305-16.

    Research output: Contribution to journalArticlepeer-review

  2. Stabilization of stalled DNA replication forks by the BRCA2 breast cancer susceptibility protein

    Lomonosov, M., Anand, S., Sangrithi, M., Davies, R. & Venkitaraman, A. R., 15 Dec 2003, In: Genes & Development. 17, 24, p. 3017-22 6 p.

    Research output: Contribution to journalArticlepeer-review

  3. Identification of WTAP, a novel Wilms' tumour 1-associating protein

    Little, N. A., Hastie, N. D. & Davies, R. C., 22 Sep 2000, In: Human Molecular Genetics. 9, 15, p. 2231-9 9 p.

    Research output: Contribution to journalArticlepeer-review

  4. Did nucleotides or amino acids drive evolutionary conservation of the WT1 +/-KTS alternative splice?

    Davies, R. C., Bratt, E. & Hastie, N. D., 1 May 2000, In: Human Molecular Genetics. 9, 8, p. 1177-83 7 p.

    Research output: Contribution to journalArticlepeer-review

  5. Multiple roles for the Wilms' tumor suppressor, WT1

    Davies, R., Moore, A., Schedl, A., Bratt, E., Miyahawa, K., Ladomery, M., Miles, C., Menke, A., van Heyningen, V. & Hastie, N., 1 Apr 1999, In: Cancer Research. 59, 7 Suppl, p. 1747s-1750s; discussion 1751s

    Research output: Contribution to journalReview articlepeer-review

Related by journal

  1. EMBO Journal (Journal)

    Helder Ferreira (Reviewer)

    2014

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. EMBO Journal (Journal)

    Silvia Paracchini (Reviewer)

    2011

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  3. EMBO Journal (Journal)

    Terry K Smith (Member of editorial board)

    2009 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. Dynamic properties of noise and Her6 levels are optimized by miR-9, allowing the decoding of the Her6 oscillator

    Soto, X., Biga, V., Kursawe, J., Lea, R., Doostdar, P., Thomas, R. & Papalopulu, N., 17 Jun 2020, In: EMBO Journal. 39, 12, 23 p., e103558.

    Research output: Contribution to journalArticlepeer-review

  2. A secretagogin locus of the mammalian hypothalamus controls stress hormone release

    Romanov, R. A., Alpár, A., Zhang, M-D., Zeisel, A., Calas, A., Landry, M., Fuszard, M. A., Shirran, S. L., Schnell, R., Dobolyi, Á., Oláh, M., Spence, L., Mulder, J., Martens, H., Palkovits, M., Uhlen, M., Sitte, H. H., Botting, C. H., Wagner, L., Linnarsson, S. & 2 others, Hökfelt, T. & Harkany, T., 2 Jan 2015, In: EMBO Journal. 34, 1, p. 36-54

    Research output: Contribution to journalArticlepeer-review

  3. mTOR activates the VPS34-UVRAG complex to regulate autolysosomal tubulation and cell survival

    Munson, M. J., Allen, G. F. G., Toth, R., Campbell, D. G., Lucocq, J. M. & Ganley, I. G., 2 Sep 2015, In: EMBO Journal. 34, 17, p. 2272-2290 19 p.

    Research output: Contribution to journalArticlepeer-review

  4. Miswiring the brain: delta(9)-tetrahydrocannabinol disrupts cortical development by inducing an SCG10/stathmin-2 degradation pathway

    Tortoriello, G., Morris, C. V., Alpar, A., Fuzik, J., Shirran, S. L., Calvigioni, D., Keimpema, E., Botting, C. H., Reinecke, K., Herdegen, T., Courtney, M., Hurd, Y. L. & Harkany, T., 1 Apr 2014, In: EMBO Journal. 33, 7, p. 668-685 18 p.

    Research output: Contribution to journalArticlepeer-review

  5. Surface dynamics of GluN2B-NMDA receptors controls plasticity of maturing glutamate synapses

    Dupuis, J. P., Ladépêche, L., Seth, H., Bard, L., Varela, J., Mikasova, L., Bouchet, D., Rogemond, V., Honnorat, J., Hanse, E. & Groc, L., 16 Apr 2014, In: EMBO Journal. 33, 8, p. 842-861 20 p.

    Research output: Contribution to journalArticlepeer-review

ID: 260533058

Top