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Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches

Research output: Contribution to journalArticlepeer-review

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Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches. / Jordan, M. D.; Raos, B. J.; Bunting, A. S.; Murray, A. F.; Graham, E. S.; Unsworth, C. P.

In: Biomaterials, Vol. 105, 10.2016, p. 117-126.

Research output: Contribution to journalArticlepeer-review

Harvard

Jordan, MD, Raos, BJ, Bunting, AS, Murray, AF, Graham, ES & Unsworth, CP 2016, 'Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches', Biomaterials, vol. 105, pp. 117-126. https://doi.org/10.1016/j.biomaterials.2016.08.006

APA

Jordan, M. D., Raos, B. J., Bunting, A. S., Murray, A. F., Graham, E. S., & Unsworth, C. P. (2016). Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches. Biomaterials, 105, 117-126. https://doi.org/10.1016/j.biomaterials.2016.08.006

Vancouver

Jordan MD, Raos BJ, Bunting AS, Murray AF, Graham ES, Unsworth CP. Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches. Biomaterials. 2016 Oct;105:117-126. https://doi.org/10.1016/j.biomaterials.2016.08.006

Author

Jordan, M. D. ; Raos, B. J. ; Bunting, A. S. ; Murray, A. F. ; Graham, E. S. ; Unsworth, C. P. / Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches. In: Biomaterials. 2016 ; Vol. 105. pp. 117-126.

Bibtex - Download

@article{0b5ee07b2ba84efdb27e9d6bcf81d0cc,
title = "Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches",
abstract = "Recent literature suggests that glia, and in particular astrocytes, should be studied as organised networks which communicate through gap junctions. Astrocytes, however, adhere to most surfaces and are highly mobile cells. In order to study, such organised networks effectively in vitro it is necessary to influence them to pattern to certain substrates whilst being repelled from others and to immobilise the astrocytes sufficiently such that they do not continue to migrate further whilst under study. In this article, we demonstrate for the first time how it is possible to facilitate the study of organised patterned human astrocytic networks using hNT astrocytes in a SiO2 trench grid network that is inlayed with the biocompatible material, parylene-C. We demonstrate how the immobilisation of astrocytes lies in the depth of the SiO2 trench, determining an optimum trench depth and that the optimum patterning of astrocytes is a consequence of the parylene-C inlay and the grid node spacing. We demonstrate high fidelity of the astrocytic networks and demonstrate that functionality of the hNT astrocytes through ATP evoked calcium signalling is also dependent on the grid node spacing. Finally, we demonstrate that the location of the nuclei on the grid nodes is also a function of the grid node spacing. The significance of this work, is to describe a suitable platform to facilitate the study of hNT astrocytes from the single cell level to the network level to improve knowledge and understanding of how communication links to spatial organisation at these higher order scales and trigger in vitro research further in this area with clinical applications in the area of epilepsy, stroke and focal cerebral ischemia.",
keywords = "Cell patterning, Human astrocyte, Astrocytic network, Parylene-C, Glia, hNT",
author = "Jordan, {M. D.} and Raos, {B. J.} and Bunting, {A. S.} and Murray, {A. F.} and Graham, {E. S.} and Unsworth, {C. P.}",
year = "2016",
month = oct,
doi = "10.1016/j.biomaterials.2016.08.006",
language = "English",
volume = "105",
pages = "117--126",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches

AU - Jordan, M. D.

AU - Raos, B. J.

AU - Bunting, A. S.

AU - Murray, A. F.

AU - Graham, E. S.

AU - Unsworth, C. P.

PY - 2016/10

Y1 - 2016/10

N2 - Recent literature suggests that glia, and in particular astrocytes, should be studied as organised networks which communicate through gap junctions. Astrocytes, however, adhere to most surfaces and are highly mobile cells. In order to study, such organised networks effectively in vitro it is necessary to influence them to pattern to certain substrates whilst being repelled from others and to immobilise the astrocytes sufficiently such that they do not continue to migrate further whilst under study. In this article, we demonstrate for the first time how it is possible to facilitate the study of organised patterned human astrocytic networks using hNT astrocytes in a SiO2 trench grid network that is inlayed with the biocompatible material, parylene-C. We demonstrate how the immobilisation of astrocytes lies in the depth of the SiO2 trench, determining an optimum trench depth and that the optimum patterning of astrocytes is a consequence of the parylene-C inlay and the grid node spacing. We demonstrate high fidelity of the astrocytic networks and demonstrate that functionality of the hNT astrocytes through ATP evoked calcium signalling is also dependent on the grid node spacing. Finally, we demonstrate that the location of the nuclei on the grid nodes is also a function of the grid node spacing. The significance of this work, is to describe a suitable platform to facilitate the study of hNT astrocytes from the single cell level to the network level to improve knowledge and understanding of how communication links to spatial organisation at these higher order scales and trigger in vitro research further in this area with clinical applications in the area of epilepsy, stroke and focal cerebral ischemia.

AB - Recent literature suggests that glia, and in particular astrocytes, should be studied as organised networks which communicate through gap junctions. Astrocytes, however, adhere to most surfaces and are highly mobile cells. In order to study, such organised networks effectively in vitro it is necessary to influence them to pattern to certain substrates whilst being repelled from others and to immobilise the astrocytes sufficiently such that they do not continue to migrate further whilst under study. In this article, we demonstrate for the first time how it is possible to facilitate the study of organised patterned human astrocytic networks using hNT astrocytes in a SiO2 trench grid network that is inlayed with the biocompatible material, parylene-C. We demonstrate how the immobilisation of astrocytes lies in the depth of the SiO2 trench, determining an optimum trench depth and that the optimum patterning of astrocytes is a consequence of the parylene-C inlay and the grid node spacing. We demonstrate high fidelity of the astrocytic networks and demonstrate that functionality of the hNT astrocytes through ATP evoked calcium signalling is also dependent on the grid node spacing. Finally, we demonstrate that the location of the nuclei on the grid nodes is also a function of the grid node spacing. The significance of this work, is to describe a suitable platform to facilitate the study of hNT astrocytes from the single cell level to the network level to improve knowledge and understanding of how communication links to spatial organisation at these higher order scales and trigger in vitro research further in this area with clinical applications in the area of epilepsy, stroke and focal cerebral ischemia.

KW - Cell patterning

KW - Human astrocyte

KW - Astrocytic network

KW - Parylene-C

KW - Glia

KW - hNT

U2 - 10.1016/j.biomaterials.2016.08.006

DO - 10.1016/j.biomaterials.2016.08.006

M3 - Article

C2 - 27521614

AN - SCOPUS:84981287690

VL - 105

SP - 117

EP - 126

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -

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