Research output: Contribution to journal › Article › peer-review
Corinna Patzina, Catherine H. Botting, Adolfo García-Sastre, Richard E. Randall, Benjamin G. Hale
NS1 proteins of influenza A and B viruses share limited sequence homology, yet both are potent manipulators of host cell processes, particularly interferon (IFN) induction. Although many cellular partners are reported for A/NS1, only a few (e.g. PKR and ISG15) have been identified for B/NS1. Here, affinity-purification and mass spectrometry were used to expand the known host interactome of B/NS1. We identified 22 human proteins as new putative targets for B/NS1, validating several, including DHX9, ILF3, YBX1 and HNRNPC. Consistent with two RNA-binding domains in B/NS1, many of the identified factors bind RNA and some interact with B/NS1 in an RNA-dependent manner. Functional characterization of several B/NS1 interactors identified SNRNP200 as a potential positive regulator of host IFN responses, while ILF3 exhibited dual roles in both IFN induction and influenza B virus replication. These data provide a resource for future investigations into the mechanisms underpinning host cell modulation by influenza B virus NS1.
Original language | English |
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Article number | 000909 |
Pages (from-to) | 2267-2273 |
Number of pages | 7 |
Journal | Journal of General Virology |
Volume | 98 |
Issue number | 9 |
DOIs | |
Publication status | Published - 1 Sep 2017 |
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Rupert James Martin Russell (Member of editorial board)
Activity: Publication peer-review and editorial work types › Editor of research journal
Richard Michael Elliott (Editor)
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Richard Michael Elliott (Member of editorial board)
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Richard Michael Elliott (Editor)
Activity: Publication peer-review and editorial work types › Editor of research journal
Richard Michael Elliott (Member of editorial board)
Activity: Publication peer-review and editorial work types › Editor of research journal
Research output: Contribution to journal › Article › peer-review
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Research output: Contribution to journal › Review article › peer-review
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