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Human papillomavirus type 16 E7 regulates E2F and contributes to mitogenic signalling

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Human papillomavirus type 16 E7 regulates E2F and contributes to mitogenic signalling. / Morris, J D; Crook, T; Bandara, L R; Davies, R; LaThangue, N B; Vousden, K H.

In: Oncogene, Vol. 8, No. 4, 04.1993, p. 893-8.

Research output: Contribution to journalArticlepeer-review

Harvard

Morris, JD, Crook, T, Bandara, LR, Davies, R, LaThangue, NB & Vousden, KH 1993, 'Human papillomavirus type 16 E7 regulates E2F and contributes to mitogenic signalling', Oncogene, vol. 8, no. 4, pp. 893-8.

APA

Morris, J. D., Crook, T., Bandara, L. R., Davies, R., LaThangue, N. B., & Vousden, K. H. (1993). Human papillomavirus type 16 E7 regulates E2F and contributes to mitogenic signalling. Oncogene, 8(4), 893-8.

Vancouver

Morris JD, Crook T, Bandara LR, Davies R, LaThangue NB, Vousden KH. Human papillomavirus type 16 E7 regulates E2F and contributes to mitogenic signalling. Oncogene. 1993 Apr;8(4):893-8.

Author

Morris, J D ; Crook, T ; Bandara, L R ; Davies, R ; LaThangue, N B ; Vousden, K H. / Human papillomavirus type 16 E7 regulates E2F and contributes to mitogenic signalling. In: Oncogene. 1993 ; Vol. 8, No. 4. pp. 893-8.

Bibtex - Download

@article{9b2963b44775423198d558274351bb34,
title = "Human papillomavirus type 16 E7 regulates E2F and contributes to mitogenic signalling",
abstract = "We have produced human papillomavirus type 16 E7 protein in a bacterial expression system and examined the mitogenic activity of this protein in Swiss 3T3 cells after scrape loading. The ability of E7 to induce cellular DNA synthesis in quiescent mouse fibroblasts is strongly enhanced by the presence of a single growth factor such as insulin. Although only weakly mitogenic, introduction of E7 alone resulted in the rapid induction of the transcriptionally active form of E2F, which was not enhanced further by the addition of insulin. Mutant E7 proteins defective for RB binding failed to induce the active form of E2F or act synergistically with insulin to stimulate DNA synthesis. The ability of E7 to regulate E2F may therefore be necessary, but is not sufficient, for full induction of DNA synthesis.",
keywords = "3T3 Cells, Animals, Base Sequence, Carrier Proteins, Cell Cycle Proteins, DNA Replication, DNA-Binding Proteins, E2F Transcription Factors, In Vitro Techniques, Insulin/pharmacology, Mice, Mitosis, Molecular Sequence Data, Oncogene Proteins, Viral/immunology, Papillomavirus E7 Proteins, Recombinant Fusion Proteins/pharmacology, Recombinant Proteins, Retinoblastoma-Binding Protein 1, Transcription Factor DP1, Transcription Factors/metabolism",
author = "Morris, {J D} and T Crook and Bandara, {L R} and R Davies and LaThangue, {N B} and Vousden, {K H}",
year = "1993",
month = apr,
language = "English",
volume = "8",
pages = "893--8",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature publishing group",
number = "4",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Human papillomavirus type 16 E7 regulates E2F and contributes to mitogenic signalling

AU - Morris, J D

AU - Crook, T

AU - Bandara, L R

AU - Davies, R

AU - LaThangue, N B

AU - Vousden, K H

PY - 1993/4

Y1 - 1993/4

N2 - We have produced human papillomavirus type 16 E7 protein in a bacterial expression system and examined the mitogenic activity of this protein in Swiss 3T3 cells after scrape loading. The ability of E7 to induce cellular DNA synthesis in quiescent mouse fibroblasts is strongly enhanced by the presence of a single growth factor such as insulin. Although only weakly mitogenic, introduction of E7 alone resulted in the rapid induction of the transcriptionally active form of E2F, which was not enhanced further by the addition of insulin. Mutant E7 proteins defective for RB binding failed to induce the active form of E2F or act synergistically with insulin to stimulate DNA synthesis. The ability of E7 to regulate E2F may therefore be necessary, but is not sufficient, for full induction of DNA synthesis.

AB - We have produced human papillomavirus type 16 E7 protein in a bacterial expression system and examined the mitogenic activity of this protein in Swiss 3T3 cells after scrape loading. The ability of E7 to induce cellular DNA synthesis in quiescent mouse fibroblasts is strongly enhanced by the presence of a single growth factor such as insulin. Although only weakly mitogenic, introduction of E7 alone resulted in the rapid induction of the transcriptionally active form of E2F, which was not enhanced further by the addition of insulin. Mutant E7 proteins defective for RB binding failed to induce the active form of E2F or act synergistically with insulin to stimulate DNA synthesis. The ability of E7 to regulate E2F may therefore be necessary, but is not sufficient, for full induction of DNA synthesis.

KW - 3T3 Cells

KW - Animals

KW - Base Sequence

KW - Carrier Proteins

KW - Cell Cycle Proteins

KW - DNA Replication

KW - DNA-Binding Proteins

KW - E2F Transcription Factors

KW - In Vitro Techniques

KW - Insulin/pharmacology

KW - Mice

KW - Mitosis

KW - Molecular Sequence Data

KW - Oncogene Proteins, Viral/immunology

KW - Papillomavirus E7 Proteins

KW - Recombinant Fusion Proteins/pharmacology

KW - Recombinant Proteins

KW - Retinoblastoma-Binding Protein 1

KW - Transcription Factor DP1

KW - Transcription Factors/metabolism

M3 - Article

C2 - 8455943

VL - 8

SP - 893

EP - 898

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 4

ER -

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