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ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae

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ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae. / Bosse, Janine T.; Li, Yanwen; Crespo, Roberto Fernandez; Chaudhuri, Roy R.; Rogers, Jon; Holden, Matthew T. G.; Maskell, Duncan J.; Tucker, Alexander W.; Wren, Brendan W.; Rycroft, Andrew N.; Langford, Paul R.; BRaDP1T Consortium.

In: Frontiers in Microbiology, Vol. 7, 810, 15.06.2016.

Research output: Contribution to journalArticle

Harvard

Bosse, JT, Li, Y, Crespo, RF, Chaudhuri, RR, Rogers, J, Holden, MTG, Maskell, DJ, Tucker, AW, Wren, BW, Rycroft, AN, Langford, PR & BRaDP1T Consortium 2016, 'ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae', Frontiers in Microbiology, vol. 7, 810. https://doi.org/10.3389/fmicb.2016.00810

APA

Bosse, J. T., Li, Y., Crespo, R. F., Chaudhuri, R. R., Rogers, J., Holden, M. T. G., ... BRaDP1T Consortium (2016). ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae. Frontiers in Microbiology, 7, [810]. https://doi.org/10.3389/fmicb.2016.00810

Vancouver

Bosse JT, Li Y, Crespo RF, Chaudhuri RR, Rogers J, Holden MTG et al. ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae. Frontiers in Microbiology. 2016 Jun 15;7. 810. https://doi.org/10.3389/fmicb.2016.00810

Author

Bosse, Janine T. ; Li, Yanwen ; Crespo, Roberto Fernandez ; Chaudhuri, Roy R. ; Rogers, Jon ; Holden, Matthew T. G. ; Maskell, Duncan J. ; Tucker, Alexander W. ; Wren, Brendan W. ; Rycroft, Andrew N. ; Langford, Paul R. ; BRaDP1T Consortium. / ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae. In: Frontiers in Microbiology. 2016 ; Vol. 7.

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@article{d8caf049b7f04291b02cf5036bd8ac54,
title = "ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae",
abstract = "ICEApl1 was identified in the whole genome sequence of MIDG2331, a tetracycline resistant (MIC = 8 mg/L) serovar 8 clinical isolate of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. PCR amplification of virB4, one of the core genes involved in conjugation, was used to identify other A. pleuropneumoniae isolates potentially carrying ICEApl1. MICs for tetracycline were determined for virB4 positive isolates, and shotgun whole genome sequence analysis was used to confirm presence of the complete ICEApl1. The sequence of ICEApl1 is 56083 bp long and contains 67 genes including a Tn10 element encoding tetracycline resistance. Comparative sequence analysis was performed with similar integrative conjugative elements (ICEs) found in other members of the Pasteurellaceae. ICEApl1 is most similar to the 59393 bp ICEHin1056, from Haemophilus influenzae strain 1056. Although initially identified only in serovar 8 isolates of A. pleuropneumoniae (31 from the UK and 1 from Cyprus), conjugal transfer of ICEApl1 to representative isolates of other serovars was confirmed. All isolates carrying ICEApl1 had a MIC for tetracycline of 8 mg/L. This is, to our knowledge, the first description of an ICE in A. pleuropneumoniae, and the first report of a member of the ICEHin1056 subfamily in a non-human pathogen. ICEApl1 confers resistance to tetracycline, currently one of the more commonly used antibiotics for treatment and control of porcine pleuropneumonia.",
keywords = "Animal infections, Antibiotic resistance, Respiratory tract, Conjugation, Pasteurellaceae",
author = "Bosse, {Janine T.} and Yanwen Li and Crespo, {Roberto Fernandez} and Chaudhuri, {Roy R.} and Jon Rogers and Holden, {Matthew T. G.} and Maskell, {Duncan J.} and Tucker, {Alexander W.} and Wren, {Brendan W.} and Rycroft, {Andrew N.} and Langford, {Paul R.} and {BRaDP1T Consortium}",
note = "This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (BBSRC grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G018553/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) Consortium. MTGH was supported by the Wellcome Trust (grant number 098051). JR was funded from the former AHVLA’s Research and Development Internal Investment Fund (grant number RD0030c).",
year = "2016",
month = "6",
day = "15",
doi = "10.3389/fmicb.2016.00810",
language = "English",
volume = "7",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae

AU - Bosse, Janine T.

AU - Li, Yanwen

AU - Crespo, Roberto Fernandez

AU - Chaudhuri, Roy R.

AU - Rogers, Jon

AU - Holden, Matthew T. G.

AU - Maskell, Duncan J.

AU - Tucker, Alexander W.

AU - Wren, Brendan W.

AU - Rycroft, Andrew N.

AU - Langford, Paul R.

AU - BRaDP1T Consortium

N1 - This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (BBSRC grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G018553/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) Consortium. MTGH was supported by the Wellcome Trust (grant number 098051). JR was funded from the former AHVLA’s Research and Development Internal Investment Fund (grant number RD0030c).

PY - 2016/6/15

Y1 - 2016/6/15

N2 - ICEApl1 was identified in the whole genome sequence of MIDG2331, a tetracycline resistant (MIC = 8 mg/L) serovar 8 clinical isolate of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. PCR amplification of virB4, one of the core genes involved in conjugation, was used to identify other A. pleuropneumoniae isolates potentially carrying ICEApl1. MICs for tetracycline were determined for virB4 positive isolates, and shotgun whole genome sequence analysis was used to confirm presence of the complete ICEApl1. The sequence of ICEApl1 is 56083 bp long and contains 67 genes including a Tn10 element encoding tetracycline resistance. Comparative sequence analysis was performed with similar integrative conjugative elements (ICEs) found in other members of the Pasteurellaceae. ICEApl1 is most similar to the 59393 bp ICEHin1056, from Haemophilus influenzae strain 1056. Although initially identified only in serovar 8 isolates of A. pleuropneumoniae (31 from the UK and 1 from Cyprus), conjugal transfer of ICEApl1 to representative isolates of other serovars was confirmed. All isolates carrying ICEApl1 had a MIC for tetracycline of 8 mg/L. This is, to our knowledge, the first description of an ICE in A. pleuropneumoniae, and the first report of a member of the ICEHin1056 subfamily in a non-human pathogen. ICEApl1 confers resistance to tetracycline, currently one of the more commonly used antibiotics for treatment and control of porcine pleuropneumonia.

AB - ICEApl1 was identified in the whole genome sequence of MIDG2331, a tetracycline resistant (MIC = 8 mg/L) serovar 8 clinical isolate of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. PCR amplification of virB4, one of the core genes involved in conjugation, was used to identify other A. pleuropneumoniae isolates potentially carrying ICEApl1. MICs for tetracycline were determined for virB4 positive isolates, and shotgun whole genome sequence analysis was used to confirm presence of the complete ICEApl1. The sequence of ICEApl1 is 56083 bp long and contains 67 genes including a Tn10 element encoding tetracycline resistance. Comparative sequence analysis was performed with similar integrative conjugative elements (ICEs) found in other members of the Pasteurellaceae. ICEApl1 is most similar to the 59393 bp ICEHin1056, from Haemophilus influenzae strain 1056. Although initially identified only in serovar 8 isolates of A. pleuropneumoniae (31 from the UK and 1 from Cyprus), conjugal transfer of ICEApl1 to representative isolates of other serovars was confirmed. All isolates carrying ICEApl1 had a MIC for tetracycline of 8 mg/L. This is, to our knowledge, the first description of an ICE in A. pleuropneumoniae, and the first report of a member of the ICEHin1056 subfamily in a non-human pathogen. ICEApl1 confers resistance to tetracycline, currently one of the more commonly used antibiotics for treatment and control of porcine pleuropneumonia.

KW - Animal infections

KW - Antibiotic resistance

KW - Respiratory tract

KW - Conjugation

KW - Pasteurellaceae

U2 - 10.3389/fmicb.2016.00810

DO - 10.3389/fmicb.2016.00810

M3 - Article

VL - 7

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 810

ER -

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