Skip to content

Research at St Andrews

Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae

Research output: Contribution to journalArticle

DOI

Open Access permissions

Open

Author(s)

Janine T Bossé, Yanwen Li, Stephanie Walker, Tom Atherton, Roberto Fernandez Crespo, Susanna M Williamson, Jon Rogers, Roy R Chaudhuri, Lucy A Weinert, Olusegun Oshota, Matthew Holden, Duncan J Maskell, Alexander W Tucker, Brendan W Wren, Andrew N Rycroft, Paul R Langford, BRaDP1T Consortium

School/Research organisations

Abstract

Objectives: The objective of this study was to determine the distribution and genetic basis of trimethoprim resistance in Actinobacillus pleuropneumoniae isolates from pigs in England.

Methods: Clinical isolates collected between 1998 and 2011 were tested for resistance to trimethoprim and sulphonamide. The genetic basis of trimethoprim resistance was determined by shotgun WGS analysis and the subsequent isolation and sequencing of plasmids.

Results: A total of 16 (out of 106) A. pleuropneumoniae isolates were resistant to both trimethoprim (MIC > 32 mg/L) and sulfisoxazole (MIC ≥ 256 mg/L), and a further 32 were resistant only to sulfisoxazole (MIC ≥256 mg/L). Genome
sequence data for the trimethoprim-resistant isolates revealed the presence of the dfrA14 dihydrofolate reductase gene. The distribution of plasmid sequences in multiple contigs suggested the presence of two distinct dfrA14-containing
plasmids in different isolates, which was confirmed by plasmid isolation and sequencing. Both plasmids encoded mobilization genes, the sulphonamide resistance gene sul2, as well as dfrA14 inserted into strA, a streptomycin-resistance-associated gene, although the gene order differed between the two plasmids. One of the plasmids further encoded the strB streptomycin-resistance-associated gene.

Conclusions: This is the first description of mobilizable plasmids conferring trimethoprim resistance in A. pleuropneumoniae and, to our knowledge, the first report of dfrA14 in any member of the Pasteurellaceae. The identification of dfrA14 conferring trimethoprim resistance in A. pleuropneumoniae isolates will facilitate PCR screens for resistance to this important antimicrobial.

Close

Details

Original languageEnglish
Pages (from-to)2217-2222
Number of pages6
JournalJournal of Antimicrobial Chemotherapy
Volume70
Issue number8
Early online date8 May 2015
DOIs
StatePublished - Aug 2015

    Research areas

  • Animal infections, Antibiotic resistance, Respiratory tract

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Global scale dissemination of ST93: a divergent Staphylococcus aureus epidemic lineage that has recently emerged from remote Northern Australia

    van Hal, S. J., Steinig, E. J., Andersson, P., Holden, M. T. G., Harris, S. R., Nimmo, G. R., Williamson, D. A., Heffernan, H., Ritchie, S. R., Kearns, A. M., Ellington, M. J., Dickson, E., de Lencastre, H., Coombs, G. W., Bentley, S. D., Parkhill, J., Holt, D. C., Giffard, P. M. & Tong, S. Y. C. 9 Jul 2018 In : Frontiers in Microbiology. 9, 11 p., 1453

    Research output: Contribution to journalArticle

  2. Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing

    Bossé, J. T., Li, Y., Fernandez Crespo, R., Lacouture, S., Gottschalk, M., Sárközi, R., Fodor, L., Casas Amoribieta, M., Angen, Ø., Nedbalcova, K., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R. & on behalf of the BRaDP1T consortium Jul 2018 In : Veterinary Microbiology. 220, p. 83-89 7 p.

    Research output: Contribution to journalArticle

  3. An outbreak of Streptococcus pyogenes in a mental health facility: advantage of well-timed whole-genome sequencing over emm typing

    Bergin, S. M. , Periaswamy, B. , Barkham, T. , Chua, H. C. , Mok, Y. M. , Fung, D. S. S. , Su, A. H. C. , Lee, Y. L. , Chua, M. L. I. , Ng, P. Y. , Soon, W. J. W. , Chu, C. W. , Tan, S. L. , Meehan, M. , Ang, B. S. P. , Leo, Y. S. , Holden, M. T. G. , De, P. , Hsu, L. Y. , Chen, S. L. & 2 others de Sessions, P. F. & Marimuthu, K. 9 May 2018 In : Infection Control and Hospital Epidemiology. First View, 9 p.

    Research output: Contribution to journalArticle

  4. The widespread use of topical antimicrobials enriches for resistance in Staphylococcus aureus isolated from Atopic Dermatitis patients

    Harkins, C. P., McAleer, M. A., Bennett, D., McHugh, M., Fleury, O. M., Pettigrew, K. A., Oravcová, K., Parkhill, J., Proby, C. M., Dawe, R. S., Geoghegan, J. A., Irvine, A. D. & Holden, M. T. G. 5 May 2018 (Accepted/In press) In : British Journal of Dermatology. In press

    Research output: Contribution to journalArticle

  5. Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA

    Hao, L., Holden, M. T. G., Wang, X., Andrew, L., Wellnitz, S., Hu, F., Whaley, M., Sammons, S., Knipe, K., Frace, M., McNamara, L. A., Liberator, P. & Anderson, A. S. Apr 2018 In : Microbial Genomics. 4, 4, 10 p., e000155

    Research output: Contribution to journalArticle

Related by journal

  1. Linezolid pharmacokinetics in MDR-TB: a systematic review, meta-analysis and Monte Carlo simulation

    Millard, J., Pertinez, H., Bonnett, L., Model, E. M., Dartois, V., Johnson, J. L., Caws, M., Tiberi, S., Bolhuis, M., Alffenaar, J-W. C., Davies, G. & Sloan, D. J. 23 Mar 2018 In : Journal of Antimicrobial Chemotherapy. 73, 7, p. 1755-1762 8 p.

    Research output: Contribution to journalArticle

  2. Has primary care antimicrobial use really been increasing? Comparison of changes in different prescribing measures for a complete geographic population 1995–2014

    Neilly, M. D. J., Guthrie, B., Hernandez Santiago, V., Vadiveloo, T., Donnan, P. T. & Marwick, C. A. 1 Oct 2017 In : Journal of Antimicrobial Chemotherapy. 72, 10, p. 2921-2930 10 p.

    Research output: Contribution to journalArticle

  3. Improved power for TB phase IIa trials using a model-based pharmacokinetic-pharmacodynamic approach compared with commonly used analysis methods

    Svensson, R. J., Gillespie, S. H. & Simonsson, U. S. H. 1 Aug 2017 In : Journal of Antimicrobial Chemotherapy. 72, 8, p. 2311-2319 9 p.

    Research output: Contribution to journalArticle

  4. ST3268: a geographically widespread primate MRSA clone

    Hsu, L-Y., Holden, M. T. G., Koh, T. H., Pettigrew, K. A., Cao, D., Hon, P. Y., Sergio, D. M., Pena, E. & Ogden, B. E. 1 Aug 2017 In : Journal of Antimicrobial Chemotherapy. 72, 8, p. 2401-2403 2 p.

    Research output: Contribution to journalLetter

  5. Early phase evaluation of SQ109 alone and in combination with rifampicin in pulmonary TB patients

    Heinrich, N., Dawson, R., du Bois, J., Narunsky, K., Horwith, G., Phipps, A. J., Nacy, C. A., Aarnoutse, R. E., Boeree, M. J., Gillespie, S. H., Venter, A., Henne, S., Rachow, A., Phillips, P. P. J., Hoelscher, M., Diacon, A. H. & on behalf of the Pan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA) 27 Jan 2015 In : Journal of Antimicrobial Chemotherapy.

    Research output: Contribution to journalArticle

ID: 186499405