Skip to content

Research at St Andrews

Identification of WTAP, a novel Wilms' tumour 1-associating protein

Research output: Contribution to journalArticlepeer-review

Author(s)

N A Little, N D Hastie, R C Davies

School/Research organisations

Abstract

The Wilms' tumour suppressor gene WT1 is essential for the normal development of the genitourinary system. It appears to play a role in both transcriptional and post-transcriptional regulation of certain cellular genes. However, the mechanisms behind WT1 function are not clearly understood despite the identification of numerous potential target genes and the isolation of several WT1-binding proteins. This study therefore sets out to identify other WT1-associating proteins to help to unravel how WT1 interacts with the cellular machinery. We report the identification of a novel human WT1-associating protein, WTAP, which was isolated using the yeast two-hybrid system. Both in vitro and in vivo assays have shown that the interaction between WTAP and WT1 is specific and occurs endogenously in cells. The mouse homologue of WTAP was isolated and found to be >90% conserved at the nucleotide and protein levels. The human and mouse genes were mapped using fluorescence in situ hybridization to regions in chromosomes 6 (which is thought to harbour a tumour suppressor gene) and 17, respectively. The expression pattern of WTAP was investigated and shown to be ubiquitous, perhaps reflecting a housekeeping role. WTAP is a nuclear protein, which like WT1 localizes throughout the nucleoplasm as well as in speckles and partially co-localizes with splicing factors. Although the significance of this interaction is not yet known, WTAP promises to be an interesting WT1-binding partner.

Close

Details

Original languageEnglish
Pages (from-to)2231-9
Number of pages9
JournalHuman Molecular Genetics
Volume9
Issue number15
DOIs
Publication statusPublished - 22 Sep 2000

    Research areas

  • Amino Acid Sequence, Animals, Carrier Proteins/genetics, Chromosome Mapping, Chromosomes, Human, Pair 6, DNA-Binding Proteins/metabolism, Genes, Wilms Tumor, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Kidney Neoplasms/genetics, Mice, Molecular Sequence Data, Nuclear Proteins/genetics, Organ Specificity, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Transcription Factors/metabolism, WT1 Proteins, Wilms Tumor/genetics

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Association of human papillomavirus 16 E2 with Rad50-interacting protein 1 enhances viral DNA replication

    Campos-León, K., Wijendra, K., Siddiqa, A., Pentland, I., Feeney, K. M., Knapman, A., Davies, R., Androphy, E. J. & Parish, J. L., Mar 2017, In: Journal of Virology. 91, 5, 18 p., e02305-16.

    Research output: Contribution to journalArticlepeer-review

  2. Stabilization of stalled DNA replication forks by the BRCA2 breast cancer susceptibility protein

    Lomonosov, M., Anand, S., Sangrithi, M., Davies, R. & Venkitaraman, A. R., 15 Dec 2003, In: Genes & Development. 17, 24, p. 3017-22 6 p.

    Research output: Contribution to journalArticlepeer-review

  3. Did nucleotides or amino acids drive evolutionary conservation of the WT1 +/-KTS alternative splice?

    Davies, R. C., Bratt, E. & Hastie, N. D., 1 May 2000, In: Human Molecular Genetics. 9, 8, p. 1177-83 7 p.

    Research output: Contribution to journalArticlepeer-review

  4. Multiple roles for the Wilms' tumor suppressor, WT1

    Davies, R., Moore, A., Schedl, A., Bratt, E., Miyahawa, K., Ladomery, M., Miles, C., Menke, A., van Heyningen, V. & Hastie, N., 1 Apr 1999, In: Cancer Research. 59, 7 Suppl, p. 1747s-1750s; discussion 1751s

    Research output: Contribution to journalReview articlepeer-review

  5. WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes

    Davies, R. C., Calvio, C., Bratt, E., Larsson, S. H., Lamond, A. I. & Hastie, N. D., 15 Oct 1998, In: Genes & Development. 12, 20, p. 3217-25 9 p.

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. Human Molecular Genetics (Journal)

    Paul Andrew Reynolds (Reviewer)

    2009 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. Human Molecular Genetics (Journal)

    Paul Andrew Reynolds (Reviewer)

    2009

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. A rare missense variant in the ATP2C2 gene is associated with language impairment and related measures

    Martinelli, A., Rice, M., Talcott, J. B., Diaz, R., Smith, S., Hashim Raza, M., Snowling, M. J., Hulme, C., Stein, J., Hayiou-Thomas, M. E., Hawi, Z., Kent, L., Pitt, S. J., Newbury, D. F. & Paracchini, S., 16 Apr 2021, In: Human Molecular Genetics. In press, ddab111.

    Research output: Contribution to journalArticlepeer-review

  2. Lamin A/C dysregulation contributes to cardiac pathology in a mouse model of severe spinal muscular atrophy"

    Šoltić, D., Shorrock, H. K., Allardyce, H., Wilson, E. L., Holt, I., Synowsky, S. A., Shirran, S. L., Parson, S., Gillingwater, T. & Fuller, H., 9 Aug 2019, In: Human Molecular Genetics. Advance Article

    Research output: Contribution to journalArticlepeer-review

  3. The handedness-associated PCSK6 locus spans an intronic promoter regulating novel transcripts

    Shore, R. J., Covill, L., Pettigrew, K. A., Brandler, W. A., Diaz Vazquez, R., Xu, Y., Tello, J., Talcott, J. B., Newbury, D. F., Stein, J., Monaco, A. P. & Paracchini, S., 1 May 2016, In: Human Molecular Genetics. 25, 9, p. 1771-1779 9 p.

    Research output: Contribution to journalArticlepeer-review

  4. The DISC1 promoter: characterization and regulation by FOXP2

    Walker, R. M., Hill, A. E., Newman, A. C., Hamilton, G., Torrance, H. S., Anderson, S. M., Ogawa, F., Derizioti, P., Nicod, J., Vernes, S. C., Fisher, S. E., Thomson, P. A., Porteous, D. J. & Evans, K. L., 1 Jul 2012, In: Human Molecular Genetics. 21, 13, p. 2862-72 11 p.

    Research output: Contribution to journalArticlepeer-review

  5. PCSK6 is associated with handedness in individuals with dyslexia

    Scerri, T. S., Brandler, W. M., Paracchini, S., Morris, A. P., Ring, S. M., Richardson, A. J., Talcott, J. B., Stein, J. & Monaco, A. P., Feb 2011, In: Human Molecular Genetics. 20, 3, p. 608-614 7 p.

    Research output: Contribution to journalArticlepeer-review

ID: 260532756

Top