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In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay.

Research output: Other contribution

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In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. / Hauton, Christopher; Smith, Valerie Jane.

2004, .

Research output: Other contribution

Harvard

Hauton, C & Smith, VJ In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay.. https://doi.org/10.1016/j.fsi.2003.12.003

APA

Hauton, C., & Smith, V. J. In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. https://doi.org/10.1016/j.fsi.2003.12.003

Vancouver

Hauton C, Smith VJ. In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. 2004. https://doi.org/10.1016/j.fsi.2003.12.003

Author

Hauton, Christopher ; Smith, Valerie Jane. / In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay.

Bibtex - Download

@misc{1db3160962b94e65b3c0a524b77b1758,
title = "In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay.",
abstract = "The neutral red uptake (NRU) cell viability assay was adapted for use with lobster Homarus gammarus (Linnaeus, 1758) granulocytes Cultured in vitro. The assay was more sensitive than the conventional trypan blue exclusion assay and facilitated a higher sample throughput than subjective microscope-based assessments of cell viability. The NRU assay was demonstrated to have a linear response from 470 to at least 126 000 cells cm(-2). It was used to investigate the acute cytotoxicity of three commercial and two candidate crustacean aquaculture immunostimulants on lobster granulocytes. All five stimulants had a cytotoxic action on the granulocytes and the toxic dose for some of these stimulants was found to be below their commercially prescribed dose. The long term energetic cost of the use of these stimulants and the concomitant potential for a reduction in growth rate Of Cultured decapod crustaceans, which is fundamental to the Success of commercial aquaculture, is identified and discussed. (C) 2004 Elsevier Ltd. All rights reserved.",
keywords = "aquaculture, crustaceans, Homarus gammarus, immunostimulation, neutral red uptake assay, CARCINUS-MAENAS L, VIBRIOSIS RESISTANCE, LITOPENAEUS-VANNAMEI, PENAEUS-MONODON, MARINE DECAPODS, SHORE CRAB, INVITRO, IMMUNOSTIMULANTS, PROPHENOLOXIDASE, VARIABILITY",
author = "Christopher Hauton and Smith, {Valerie Jane}",
note = "Fish and Shellfish Immunology",
year = "2004",
month = "7",
doi = "10.1016/j.fsi.2003.12.003",
language = "English",
volume = "17",
type = "Other",

}

RIS (suitable for import to EndNote) - Download

TY - GEN

T1 - In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay.

AU - Hauton, Christopher

AU - Smith, Valerie Jane

N1 - Fish and Shellfish Immunology

PY - 2004/7

Y1 - 2004/7

N2 - The neutral red uptake (NRU) cell viability assay was adapted for use with lobster Homarus gammarus (Linnaeus, 1758) granulocytes Cultured in vitro. The assay was more sensitive than the conventional trypan blue exclusion assay and facilitated a higher sample throughput than subjective microscope-based assessments of cell viability. The NRU assay was demonstrated to have a linear response from 470 to at least 126 000 cells cm(-2). It was used to investigate the acute cytotoxicity of three commercial and two candidate crustacean aquaculture immunostimulants on lobster granulocytes. All five stimulants had a cytotoxic action on the granulocytes and the toxic dose for some of these stimulants was found to be below their commercially prescribed dose. The long term energetic cost of the use of these stimulants and the concomitant potential for a reduction in growth rate Of Cultured decapod crustaceans, which is fundamental to the Success of commercial aquaculture, is identified and discussed. (C) 2004 Elsevier Ltd. All rights reserved.

AB - The neutral red uptake (NRU) cell viability assay was adapted for use with lobster Homarus gammarus (Linnaeus, 1758) granulocytes Cultured in vitro. The assay was more sensitive than the conventional trypan blue exclusion assay and facilitated a higher sample throughput than subjective microscope-based assessments of cell viability. The NRU assay was demonstrated to have a linear response from 470 to at least 126 000 cells cm(-2). It was used to investigate the acute cytotoxicity of three commercial and two candidate crustacean aquaculture immunostimulants on lobster granulocytes. All five stimulants had a cytotoxic action on the granulocytes and the toxic dose for some of these stimulants was found to be below their commercially prescribed dose. The long term energetic cost of the use of these stimulants and the concomitant potential for a reduction in growth rate Of Cultured decapod crustaceans, which is fundamental to the Success of commercial aquaculture, is identified and discussed. (C) 2004 Elsevier Ltd. All rights reserved.

KW - aquaculture

KW - crustaceans

KW - Homarus gammarus

KW - immunostimulation

KW - neutral red uptake assay

KW - CARCINUS-MAENAS L

KW - VIBRIOSIS RESISTANCE

KW - LITOPENAEUS-VANNAMEI

KW - PENAEUS-MONODON

KW - MARINE DECAPODS

KW - SHORE CRAB

KW - INVITRO

KW - IMMUNOSTIMULANTS

KW - PROPHENOLOXIDASE

KW - VARIABILITY

UR - http://www.scopus.com/inward/record.url?scp=3042693346&partnerID=8YFLogxK

U2 - 10.1016/j.fsi.2003.12.003

DO - 10.1016/j.fsi.2003.12.003

M3 - Other contribution

VL - 17

ER -

ID: 307924