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In vivo effects of 17β-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart

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In vivo effects of 17β-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart. / Obradovic, Milan; Stewart, Alan J.; Pitt, Samantha J; Labudovic-Borovic, Milica; Sudar, Emina; Petrovic, Voin; Zafirovic, Sonja; Maravic-Stojkovic, Vera; Vasic, Vesna; Isenovic, Esma R.

In: Molecular and Cellular Endocrinology, Vol. 388, No. 1-2, 05.05.2014, p. 58-68.

Research output: Contribution to journalArticle

Harvard

Obradovic, M, Stewart, AJ, Pitt, SJ, Labudovic-Borovic, M, Sudar, E, Petrovic, V, Zafirovic, S, Maravic-Stojkovic, V, Vasic, V & Isenovic, ER 2014, 'In vivo effects of 17β-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart', Molecular and Cellular Endocrinology, vol. 388, no. 1-2, pp. 58-68. https://doi.org/10.1016/j.mce.2014.03.005

APA

Obradovic, M., Stewart, A. J., Pitt, S. J., Labudovic-Borovic, M., Sudar, E., Petrovic, V., ... Isenovic, E. R. (2014). In vivo effects of 17β-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart. Molecular and Cellular Endocrinology, 388(1-2), 58-68. https://doi.org/10.1016/j.mce.2014.03.005

Vancouver

Obradovic M, Stewart AJ, Pitt SJ, Labudovic-Borovic M, Sudar E, Petrovic V et al. In vivo effects of 17β-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart. Molecular and Cellular Endocrinology. 2014 May 5;388(1-2):58-68. https://doi.org/10.1016/j.mce.2014.03.005

Author

Obradovic, Milan ; Stewart, Alan J. ; Pitt, Samantha J ; Labudovic-Borovic, Milica ; Sudar, Emina ; Petrovic, Voin ; Zafirovic, Sonja ; Maravic-Stojkovic, Vera ; Vasic, Vesna ; Isenovic, Esma R. / In vivo effects of 17β-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart. In: Molecular and Cellular Endocrinology. 2014 ; Vol. 388, No. 1-2. pp. 58-68.

Bibtex - Download

@article{20d1bb3349b047a18a7e321084dd9686,
title = "In vivo effects of 17β-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart",
abstract = "In this study the in vivo effects of estradiol in regulating Na+/K+-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 µg/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na+/K+-ATPase activity, expression of the α1 subunit, and phosphorylation of the α1 subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na+ levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na+/K+-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na+/K+-ATPase expression and activity in rat heart.",
keywords = "Estradiol, Sodium potassium adenosine-triphosphatase, Protein kinase B, Extracellular-signal-regulated kinase ½",
author = "Milan Obradovic and Stewart, {Alan J.} and Pitt, {Samantha J} and Milica Labudovic-Borovic and Emina Sudar and Voin Petrovic and Sonja Zafirovic and Vera Maravic-Stojkovic and Vesna Vasic and Isenovic, {Esma R}",
note = "This work is supported by the Grant No. 173033 (to E.R.I) from the Ministry of Science, Republic of Serbia.",
year = "2014",
month = "5",
day = "5",
doi = "10.1016/j.mce.2014.03.005",
language = "English",
volume = "388",
pages = "58--68",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - In vivo effects of 17β-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart

AU - Obradovic, Milan

AU - Stewart, Alan J.

AU - Pitt, Samantha J

AU - Labudovic-Borovic, Milica

AU - Sudar, Emina

AU - Petrovic, Voin

AU - Zafirovic, Sonja

AU - Maravic-Stojkovic, Vera

AU - Vasic, Vesna

AU - Isenovic, Esma R

N1 - This work is supported by the Grant No. 173033 (to E.R.I) from the Ministry of Science, Republic of Serbia.

PY - 2014/5/5

Y1 - 2014/5/5

N2 - In this study the in vivo effects of estradiol in regulating Na+/K+-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 µg/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na+/K+-ATPase activity, expression of the α1 subunit, and phosphorylation of the α1 subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na+ levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na+/K+-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na+/K+-ATPase expression and activity in rat heart.

AB - In this study the in vivo effects of estradiol in regulating Na+/K+-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 µg/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na+/K+-ATPase activity, expression of the α1 subunit, and phosphorylation of the α1 subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na+ levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na+/K+-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na+/K+-ATPase expression and activity in rat heart.

KW - Estradiol

KW - Sodium potassium adenosine-triphosphatase

KW - Protein kinase B

KW - Extracellular-signal-regulated kinase ½

U2 - 10.1016/j.mce.2014.03.005

DO - 10.1016/j.mce.2014.03.005

M3 - Article

VL - 388

SP - 58

EP - 68

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 1-2

ER -

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