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Independent optical excitation of distinct neural populations

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Independent optical excitation of distinct neural populations. / Klapoetke, Nathan C; Murata, Yasunobu; Kim, Sung Soo; Pulver, Stefan R; Birdsey-Benson, Amanda; Cho, Yong Ku; Morimoto, Tania K; Chuong, Amy S; Carpenter, Eric J; Tian, Zhijian; Wang, Jun; Xie, Yinlong; Yan, Zhixiang; Zhang, Yong; Chow, Brian Y; Surek, Barbara; Melkonian, Michael; Jayaraman, Vivek; Constantine-Paton, Martha; Wong, Gane Ka-Shu; Boyden, Edward S.

In: Nature Methods, Vol. 11, No. 3, 03.2014, p. 338-346.

Research output: Contribution to journalArticlepeer-review

Harvard

Klapoetke, NC, Murata, Y, Kim, SS, Pulver, SR, Birdsey-Benson, A, Cho, YK, Morimoto, TK, Chuong, AS, Carpenter, EJ, Tian, Z, Wang, J, Xie, Y, Yan, Z, Zhang, Y, Chow, BY, Surek, B, Melkonian, M, Jayaraman, V, Constantine-Paton, M, Wong, GK-S & Boyden, ES 2014, 'Independent optical excitation of distinct neural populations', Nature Methods, vol. 11, no. 3, pp. 338-346. https://doi.org/10.1038/nmeth.2836

APA

Klapoetke, N. C., Murata, Y., Kim, S. S., Pulver, S. R., Birdsey-Benson, A., Cho, Y. K., Morimoto, T. K., Chuong, A. S., Carpenter, E. J., Tian, Z., Wang, J., Xie, Y., Yan, Z., Zhang, Y., Chow, B. Y., Surek, B., Melkonian, M., Jayaraman, V., Constantine-Paton, M., ... Boyden, E. S. (2014). Independent optical excitation of distinct neural populations. Nature Methods, 11(3), 338-346. https://doi.org/10.1038/nmeth.2836

Vancouver

Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK et al. Independent optical excitation of distinct neural populations. Nature Methods. 2014 Mar;11(3):338-346. https://doi.org/10.1038/nmeth.2836

Author

Klapoetke, Nathan C ; Murata, Yasunobu ; Kim, Sung Soo ; Pulver, Stefan R ; Birdsey-Benson, Amanda ; Cho, Yong Ku ; Morimoto, Tania K ; Chuong, Amy S ; Carpenter, Eric J ; Tian, Zhijian ; Wang, Jun ; Xie, Yinlong ; Yan, Zhixiang ; Zhang, Yong ; Chow, Brian Y ; Surek, Barbara ; Melkonian, Michael ; Jayaraman, Vivek ; Constantine-Paton, Martha ; Wong, Gane Ka-Shu ; Boyden, Edward S. / Independent optical excitation of distinct neural populations. In: Nature Methods. 2014 ; Vol. 11, No. 3. pp. 338-346.

Bibtex - Download

@article{35321cf79ebc46e9af979f2a8f3ad32c,
title = "Independent optical excitation of distinct neural populations",
abstract = "Optogenetic tools enable examination of how specific cell types contribute to brain circuit functions. A long-standing question is whether it is possible to independently activate two distinct neural populations in mammalian brain tissue. Such a capability would enable the study of how different synapses or pathways interact to encode information in the brain. Here we describe two channelrhodopsins, Chronos and Chrimson, discovered through sequencing and physiological characterization of opsins from over 100 species of alga. Chrimson's excitation spectrum is red shifted by 45 nm relative to previous channelrhodopsins and can enable experiments in which red light is preferred. We show minimal visual system–mediated behavioral interference when using Chrimson in neurobehavioral studies in Drosophila melanogaster. Chronos has faster kinetics than previous channelrhodopsins yet is effectively more light sensitive. Together these two reagents enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice.",
keywords = "Animals, Drosophila Proteins, Drosophila melanogaster, Light, Molecular Sequence Data, Neurons, Optogenetics, Rhodopsin",
author = "Klapoetke, {Nathan C} and Yasunobu Murata and Kim, {Sung Soo} and Pulver, {Stefan R} and Amanda Birdsey-Benson and Cho, {Yong Ku} and Morimoto, {Tania K} and Chuong, {Amy S} and Carpenter, {Eric J} and Zhijian Tian and Jun Wang and Yinlong Xie and Zhixiang Yan and Yong Zhang and Chow, {Brian Y} and Barbara Surek and Michael Melkonian and Vivek Jayaraman and Martha Constantine-Paton and Wong, {Gane Ka-Shu} and Boyden, {Edward S}",
year = "2014",
month = mar,
doi = "10.1038/nmeth.2836",
language = "English",
volume = "11",
pages = "338--346",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature publishing group",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Independent optical excitation of distinct neural populations

AU - Klapoetke, Nathan C

AU - Murata, Yasunobu

AU - Kim, Sung Soo

AU - Pulver, Stefan R

AU - Birdsey-Benson, Amanda

AU - Cho, Yong Ku

AU - Morimoto, Tania K

AU - Chuong, Amy S

AU - Carpenter, Eric J

AU - Tian, Zhijian

AU - Wang, Jun

AU - Xie, Yinlong

AU - Yan, Zhixiang

AU - Zhang, Yong

AU - Chow, Brian Y

AU - Surek, Barbara

AU - Melkonian, Michael

AU - Jayaraman, Vivek

AU - Constantine-Paton, Martha

AU - Wong, Gane Ka-Shu

AU - Boyden, Edward S

PY - 2014/3

Y1 - 2014/3

N2 - Optogenetic tools enable examination of how specific cell types contribute to brain circuit functions. A long-standing question is whether it is possible to independently activate two distinct neural populations in mammalian brain tissue. Such a capability would enable the study of how different synapses or pathways interact to encode information in the brain. Here we describe two channelrhodopsins, Chronos and Chrimson, discovered through sequencing and physiological characterization of opsins from over 100 species of alga. Chrimson's excitation spectrum is red shifted by 45 nm relative to previous channelrhodopsins and can enable experiments in which red light is preferred. We show minimal visual system–mediated behavioral interference when using Chrimson in neurobehavioral studies in Drosophila melanogaster. Chronos has faster kinetics than previous channelrhodopsins yet is effectively more light sensitive. Together these two reagents enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice.

AB - Optogenetic tools enable examination of how specific cell types contribute to brain circuit functions. A long-standing question is whether it is possible to independently activate two distinct neural populations in mammalian brain tissue. Such a capability would enable the study of how different synapses or pathways interact to encode information in the brain. Here we describe two channelrhodopsins, Chronos and Chrimson, discovered through sequencing and physiological characterization of opsins from over 100 species of alga. Chrimson's excitation spectrum is red shifted by 45 nm relative to previous channelrhodopsins and can enable experiments in which red light is preferred. We show minimal visual system–mediated behavioral interference when using Chrimson in neurobehavioral studies in Drosophila melanogaster. Chronos has faster kinetics than previous channelrhodopsins yet is effectively more light sensitive. Together these two reagents enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice.

KW - Animals

KW - Drosophila Proteins

KW - Drosophila melanogaster

KW - Light

KW - Molecular Sequence Data

KW - Neurons

KW - Optogenetics

KW - Rhodopsin

U2 - 10.1038/nmeth.2836

DO - 10.1038/nmeth.2836

M3 - Article

C2 - 24509633

VL - 11

SP - 338

EP - 346

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 3

ER -

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  1. Addendum: independent optical excitation of distinct neural populations

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ID: 167959560

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