Skip to content

Research at St Andrews

In-lipid structure of pressure sensitive domains hints mechanosensitive channel functional diversity

Research output: Contribution to journalArticle

Abstract

The mechanosensitive channel of large conductance (MscL) from Mycobacterium tuberculosis has been used as structural model for rationalizing functional observations in multiple MscL orthologues. Although these orthologues adopt similar structural architectures, they reportedly present significant functional differences. Subtle structural discrepancies on mechanosensitive channel nano-pockets are known to affect mechanical gating and may be linked to large variability in tension sensitivity among these membrane channels. Here we modify the nano-pocket regions of MscL from Escherichia coli and Mycobacterium tuberculosis and employ PELDOR/DEER distance and 3pESEEM deuterium accessibility measurements to interrogate channel structure within lipids, in which both channels adopt a closed conformation. Significant in-lipid structural differences between the two constructs suggest a more compact EcMscL at the membrane inner-leaflet, as a consequence of a rotated TM2 helix. Observed differences within lipids could explain EcMscL’s higher tension sensitivity and should be taken into account in extrapolated models used for MscL gating rationalization.
Close

Details

Original languageEnglish
Pages (from-to)448-459
JournalBiophysical Journal
Volume119
Issue number2
Early online date23 Jun 2020
DOIs
Publication statusPublished - 21 Jul 2020

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets

    Kapsalis, C., Wang, B., El Mkami, H., Pitt, S. J., Schnell, J., Smith, T. K., Lippiat, J., Bode, B. E. & Pliotas, C., 10 Oct 2019, In : Nature Communications. 10, 14 p., 4619.

    Research output: Contribution to journalArticle

  2. Sparse labeling PELDOR spectroscopy on multimeric mechanosensitive membrane channels

    Ackermann, K., Pliotas, C., Valera, S., Naismith, J. & Bode, B. E., 7 Nov 2017, In : Biophysical Journal. 113, 9, p. 1968–1978

    Research output: Contribution to journalArticle

  3. Accurate extraction of nanometer distances in multimers by pulse EPR

    Valera, S., Ackermann, K., Pliotas, C., Huang, H., Naismith, J. H. & Bode, B. E., 16 Mar 2016, In : Chemistry - A European Journal. 22, 14, p. 4700–4703

    Research output: Contribution to journalArticle

  4. Site-specific iron substitution in STA-28, a large pore aluminophosphate zeotype prepared using 1,10-phenanthrolines as framework-bound templates

    Watts, A. E., Lozinska, M. M., Slawin, A. M. Z., Mayoral, A., Dawson, D. M., Ashbrook, S. E., Bode, B. E., Dugulan, I., Shannon, M., Cox, P., Turrina, A. & Wright, P. A., 8 Jun 2020, In : Angewandte Chemie International Edition. Early View, 6 p.

    Research output: Contribution to journalArticle

  5. Deoxyfluorination using CuF2: enabled by a Lewis base activating group strategy

    Sood, D. E., Champion, S., Dawson, D. M., Chabbra, S., Bode, B. E., Sutherland, A. C. & Watson, A. J. B., 18 Mar 2020, In : Angewandte Chemie International Edition. Early View

    Research output: Contribution to journalArticle

Related by journal

  1. Twin-FRET: A New Molecular Ruler for Biomolecules

    Jana, S., Diez-Castellnou, M., Kay, E. R. & Penedo, C., 15 Feb 2019, In : Biophysical Journal. 116, 3, p. 565A-565A 1 p.

    Research output: Contribution to journalAbstract

  2. Analysis of the precision, robustness and speed of elastic resonator interference stress microscopy

    Liehm, P., Kronenberg, N. M. & Gather, M. C., 8 May 2018, In : Biophysical Journal. 114, 9, p. 2180-2193 14 p.

    Research output: Contribution to journalArticle

  3. Sparse labeling PELDOR spectroscopy on multimeric mechanosensitive membrane channels

    Ackermann, K., Pliotas, C., Valera, S., Naismith, J. & Bode, B. E., 7 Nov 2017, In : Biophysical Journal. 113, 9, p. 1968–1978

    Research output: Contribution to journalArticle

  4. Ultrahigh-resolution optical coherence elastography images cellular-scale stiffness of mouse aorta

    Wijesinghe, P., Johansen, N. J., Curatolo, A., Sampson, D. D., Ganss, R. & Kennedy, B. F., 5 Dec 2017, In : Biophysical Journal. 113, 11, p. 2540-2551 12 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Biophysical Journal (Journal)

    Helder Ferreira (Reviewer)

    2016

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

ID: 268446005

Top