Skip to content

Research at St Andrews

Insights into the low-temperature adaptation and nutritional flexibility of a soil-persistent Escherichia coli

Research output: Contribution to journalArticle

DOI

Author(s)

Fiona P. Brennan, Jim Grant, Catherine Helen Botting, Vincent O'Flaherty, Karl G. Richards, Florence Abram

School/Research organisations

Abstract

An understanding of the survival capacity of Escherichia coli in soil is critical for the evaluation of its role as a faecal indicator. Recent reports that E.coli can become long-term residents in maritime temperate soils have raised the question of how the organism survives and competes for niche space in the suboptimal soil environment. The ability of an environmental isolate to utilize 380 substrates was assessed together with that of a reference laboratory strain (E.coli K12) at both 15 and 37 degrees C. At 15 degrees C, the environmental strain could utilize 161 substrates, with only 67 utilizable by the reference strain, while at 37 degrees C, 239 and 223 substrates could be utilized by each strain respectively. An investigation into the cold response of the strains revealed that E.coli K12 was found to reduce the expression of biosynthetic proteins at 15 degrees C, while the environmental isolate seemed to switch on proteins involved in stress response, suggesting low-temperature adaptation in the latter. Taken together, the results indicate that the environmentally persistent E.coli strain is well adapted to use a wide range of nutrient sources at 15 degrees C and to direct its protein expression to maintain a relatively fast growth rate at low temperature.

Close

Details

Original languageEnglish
Pages (from-to)75-85
Number of pages11
JournalFEMS Microbiology Ecology
Volume84
Issue number1
Early online date27 Nov 2012
DOIs
Publication statusPublished - Apr 2013

    Research areas

  • environmental E, SURVIVAL, K-12, LISTERIA-MONOCYTOGENES, cold adaptation, GENE, AUTOINDUCER-2, POPULATION-STRUCTURE, WATER-QUALITY, ENTEROCOCCI, coli, SUBTROPICAL ENVIRONMENT, SALMONELLA, proteomics, phenotypic microarrays

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Life-long epigenetic programming of cortical architecture by maternal ‘Western’ diet during pregnancy

    Cinquina, V., Calvigioni, D., Farlik, M., Halbritter, F., Fife-Gernedl, V., Shirran, S. L., Fuszard, M. A., Botting, C. H., Poullet, P., Piscitelli, F., Máte, Z., Szabo, G., Yanagawa, Y., Kasper, S., Di Marzo, V., Mackie, K., McBain, C., Bock, C., Keimpema, E. & Harkany, T., 18 Nov 2019, In : Molecular Psychiatry. 15 p.

    Research output: Contribution to journalArticle

  2. Insights into the mechanism of the cyanobactin heterocyclase enzyme

    Ge, Y., Czekster, C. M., Miller, O. K., Botting, C. H., Schwarz-Linek, U. & Naismith, J. H., 23 Apr 2019, In : Biochemistry. 58, 16, p. 2125-2132 8 p.

    Research output: Contribution to journalArticle

  3. Identification of synovial fluid proteins that are associated with early osteoarthritis treatment failure: the search for novel markers leads us back to matrix metalloproteinases (MMPs)

    Hulme, C. H., Wilson, E., Fuller, H. R., Roberts, S., Richardson, J. B., Gallacher, P., Peffers, M. J., Shirran, S. L., Botting, C. H. & Wright, K. T., Dec 2018, In : International journal of experimental pathology. 99, 6, p. A2-A2 1 p.

    Research output: Contribution to journalAbstract

  4. Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration

    Arya, S., Emri, E., Synowsky, S. A., Shirran, S. L., Barzegar-Befroei, N., Peto, T., Botting, C. H., Lengyel, I. & Stewart, A. J., Jul 2018, In : Experimental Eye Research. 172, p. 21-29 9 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Functional responses and adaptation of mesophilic microbial communities to psychrophilic anaerobic digestion

    Gunnigle, E., Nielsen, J. L., Fuszard, M., Botting, C. H., Sheahan, J., O'Flaherty, V. & Abram, F., Dec 2015, In : FEMS Microbiology Ecology. 91, 12, 15 p., 132.

    Research output: Contribution to journalArticle

  2. Dissecting the deep biosphere: retrieving authentic microbial communities from packer-isolated deep crystalline bedrock fracture zones

    Purkamo, L., Bomberg, M., Nyyssönen, M., Kukkonen, I., Ahonen, L., Kietäväinen, R. & Itävaara, M., 1 Aug 2013, In : FEMS Microbiology Ecology. 85, 2, p. 324-37 14 p., 85.

    Research output: Contribution to journalArticle

  3. The engineering potential of natural benthic bacterial assemblages in terms of the erosion resistance of sediments

    Gerbersdorf, S. U., Manz, W. & Paterson, D. M., Nov 2008, In : FEMS Microbiology Ecology. 66, p. 282-294 13 p.

    Research output: Contribution to journalArticle

  4. Siderophore-mediated cooperation and virulence in Pseudomonas aeruginosa

    Buckling, A., Harrison, F., Vos, M., Brockhurst, M. A., Gardner, A., West, S. A. & Griffin, A., Nov 2007, In : FEMS Microbiology Ecology. 62, 2, p. 135-141 7 p.

    Research output: Contribution to journalArticle

ID: 54540578

Top