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Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells

Research output: Contribution to journalArticlepeer-review

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Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells. / Mthunzi, Patience; Lee, Woei Ming; Riches, Andrew Clive; Brown, Christian Tom A.; Gunn-Moore, Frank J.; Dholakia, Kishan.

In: IEEE Journal of Selected Topics in Quantum Electronics, Vol. 16, No. 3, 2010, p. 608-618.

Research output: Contribution to journalArticlepeer-review

Harvard

Mthunzi, P, Lee, WM, Riches, AC, Brown, CTA, Gunn-Moore, FJ & Dholakia, K 2010, 'Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells', IEEE Journal of Selected Topics in Quantum Electronics, vol. 16, no. 3, pp. 608-618. https://doi.org/10.1109/JSTQE.2009.2031313

APA

Mthunzi, P., Lee, W. M., Riches, A. C., Brown, C. T. A., Gunn-Moore, F. J., & Dholakia, K. (2010). Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells. IEEE Journal of Selected Topics in Quantum Electronics, 16(3), 608-618. https://doi.org/10.1109/JSTQE.2009.2031313

Vancouver

Mthunzi P, Lee WM, Riches AC, Brown CTA, Gunn-Moore FJ, Dholakia K. Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells. IEEE Journal of Selected Topics in Quantum Electronics. 2010;16(3):608-618. https://doi.org/10.1109/JSTQE.2009.2031313

Author

Mthunzi, Patience ; Lee, Woei Ming ; Riches, Andrew Clive ; Brown, Christian Tom A. ; Gunn-Moore, Frank J. ; Dholakia, Kishan. / Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells. In: IEEE Journal of Selected Topics in Quantum Electronics. 2010 ; Vol. 16, No. 3. pp. 608-618.

Bibtex - Download

@article{f8f1a071e76a41a18d9a42ec8984532e,
title = "Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells",
abstract = "Optical micromanipulation of transparent microparticles such as cellular materials relies upon the application of optical forces that are crucially dependent on the refractive index contrast between the particle and the surrounding medium. We briefly review the application of optical forces for cell manipulation and sorting, highlighting some of the key experiments over the last twenty years. We then introduce a new technique for enhancing the dielectric contrast of mammalian cells, which is a result of cells naturally taking up microspheres from their environment. We explore how these intracellular dielectric tags can influence the scattering and gradient forces upon these cells from an externally applied optical field. We show that intracellular polymer microspheres can serve as highly directional optical scatterers and that scattering forces can enable sorting through axial guiding onto laminin-coated glass coverslips upon which the selected cells adhere. Such internal dielectric tagging presents a simple, inexpensive, sterile technique to enhance optical manipulation procedures for cellular material and may enable new sorting techniques within microfluidic systems.",
keywords = "Intracellular dielectric tagging, optical cell sorting and axial and scattering forces, RED-BLOOD-CELLS, SINGLE CELLS, TRAPPING EFFICIENCY, ESCHERICHIA-COLI, HIGH-RESOLUTION, LIGHT FIELDS, LASER TRAP, TWEEZERS, PARTICLES, SEPARATION",
author = "Patience Mthunzi and Lee, {Woei Ming} and Riches, {Andrew Clive} and Brown, {Christian Tom A.} and Gunn-Moore, {Frank J.} and Kishan Dholakia",
year = "2010",
doi = "10.1109/JSTQE.2009.2031313",
language = "English",
volume = "16",
pages = "608--618",
journal = "IEEE Journal of Selected Topics in Quantum Electronics",
issn = "1077-260X",
publisher = "IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells

AU - Mthunzi, Patience

AU - Lee, Woei Ming

AU - Riches, Andrew Clive

AU - Brown, Christian Tom A.

AU - Gunn-Moore, Frank J.

AU - Dholakia, Kishan

PY - 2010

Y1 - 2010

N2 - Optical micromanipulation of transparent microparticles such as cellular materials relies upon the application of optical forces that are crucially dependent on the refractive index contrast between the particle and the surrounding medium. We briefly review the application of optical forces for cell manipulation and sorting, highlighting some of the key experiments over the last twenty years. We then introduce a new technique for enhancing the dielectric contrast of mammalian cells, which is a result of cells naturally taking up microspheres from their environment. We explore how these intracellular dielectric tags can influence the scattering and gradient forces upon these cells from an externally applied optical field. We show that intracellular polymer microspheres can serve as highly directional optical scatterers and that scattering forces can enable sorting through axial guiding onto laminin-coated glass coverslips upon which the selected cells adhere. Such internal dielectric tagging presents a simple, inexpensive, sterile technique to enhance optical manipulation procedures for cellular material and may enable new sorting techniques within microfluidic systems.

AB - Optical micromanipulation of transparent microparticles such as cellular materials relies upon the application of optical forces that are crucially dependent on the refractive index contrast between the particle and the surrounding medium. We briefly review the application of optical forces for cell manipulation and sorting, highlighting some of the key experiments over the last twenty years. We then introduce a new technique for enhancing the dielectric contrast of mammalian cells, which is a result of cells naturally taking up microspheres from their environment. We explore how these intracellular dielectric tags can influence the scattering and gradient forces upon these cells from an externally applied optical field. We show that intracellular polymer microspheres can serve as highly directional optical scatterers and that scattering forces can enable sorting through axial guiding onto laminin-coated glass coverslips upon which the selected cells adhere. Such internal dielectric tagging presents a simple, inexpensive, sterile technique to enhance optical manipulation procedures for cellular material and may enable new sorting techniques within microfluidic systems.

KW - Intracellular dielectric tagging

KW - optical cell sorting and axial and scattering forces

KW - RED-BLOOD-CELLS

KW - SINGLE CELLS

KW - TRAPPING EFFICIENCY

KW - ESCHERICHIA-COLI

KW - HIGH-RESOLUTION

KW - LIGHT FIELDS

KW - LASER TRAP

KW - TWEEZERS

KW - PARTICLES

KW - SEPARATION

UR - http://www.scopus.com/inward/record.url?scp=77953288500&partnerID=8YFLogxK

U2 - 10.1109/JSTQE.2009.2031313

DO - 10.1109/JSTQE.2009.2031313

M3 - Article

VL - 16

SP - 608

EP - 618

JO - IEEE Journal of Selected Topics in Quantum Electronics

JF - IEEE Journal of Selected Topics in Quantum Electronics

SN - 1077-260X

IS - 3

ER -

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ID: 1699046

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