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Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection

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Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection. / Tang, Anna Ah-San; Tiede, Christian; Hughes, David J; McPherson, Michael J; Tomlinson, Darren C.

In: Science Signaling, Vol. 10, No. 505, eaan0868, 14.11.2017.

Research output: Contribution to journalArticlepeer-review

Harvard

Tang, AA-S, Tiede, C, Hughes, DJ, McPherson, MJ & Tomlinson, DC 2017, 'Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection', Science Signaling, vol. 10, no. 505, eaan0868. https://doi.org/10.1126/scisignal.aan0868

APA

Tang, A. A-S., Tiede, C., Hughes, D. J., McPherson, M. J., & Tomlinson, D. C. (2017). Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection. Science Signaling, 10(505), [eaan0868]. https://doi.org/10.1126/scisignal.aan0868

Vancouver

Tang AA-S, Tiede C, Hughes DJ, McPherson MJ, Tomlinson DC. Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection. Science Signaling. 2017 Nov 14;10(505). eaan0868. https://doi.org/10.1126/scisignal.aan0868

Author

Tang, Anna Ah-San ; Tiede, Christian ; Hughes, David J ; McPherson, Michael J ; Tomlinson, Darren C. / Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection. In: Science Signaling. 2017 ; Vol. 10, No. 505.

Bibtex - Download

@article{31747c3ba460448f86a33a44374cfd49,
title = "Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection",
abstract = "Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding proteins. Artificial binding proteins can be used as reagents to study protein-protein interactions, target posttranslational modifications, and distinguish between homologous proteins. They can also be used as research and affinity reagents, for diagnostic purposes, and as therapeutics. However, the ability to identify isoform-specific reagents remains highly challenging. We describe an adapted phage display protocol using an artificial binding protein (Affimer) for the selection of isoform-selective binding proteins.",
author = "Tang, {Anna Ah-San} and Christian Tiede and Hughes, {David J} and McPherson, {Michael J} and Tomlinson, {Darren C}",
note = "This work was funded by the University of Leeds.",
year = "2017",
month = nov,
day = "14",
doi = "10.1126/scisignal.aan0868",
language = "English",
volume = "10",
journal = "Science Signaling",
issn = "1945-0877",
publisher = "American Association for the Advancement of Science",
number = "505",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection

AU - Tang, Anna Ah-San

AU - Tiede, Christian

AU - Hughes, David J

AU - McPherson, Michael J

AU - Tomlinson, Darren C

N1 - This work was funded by the University of Leeds.

PY - 2017/11/14

Y1 - 2017/11/14

N2 - Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding proteins. Artificial binding proteins can be used as reagents to study protein-protein interactions, target posttranslational modifications, and distinguish between homologous proteins. They can also be used as research and affinity reagents, for diagnostic purposes, and as therapeutics. However, the ability to identify isoform-specific reagents remains highly challenging. We describe an adapted phage display protocol using an artificial binding protein (Affimer) for the selection of isoform-selective binding proteins.

AB - Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding proteins. Artificial binding proteins can be used as reagents to study protein-protein interactions, target posttranslational modifications, and distinguish between homologous proteins. They can also be used as research and affinity reagents, for diagnostic purposes, and as therapeutics. However, the ability to identify isoform-specific reagents remains highly challenging. We describe an adapted phage display protocol using an artificial binding protein (Affimer) for the selection of isoform-selective binding proteins.

U2 - 10.1126/scisignal.aan0868

DO - 10.1126/scisignal.aan0868

M3 - Article

C2 - 29138294

VL - 10

JO - Science Signaling

JF - Science Signaling

SN - 1945-0877

IS - 505

M1 - eaan0868

ER -

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