Skip to content

Research at St Andrews

L1Tc non-LTR retrotransposons from Trypanosoma cruzi contain a functional viral-like self-cleaving 2A sequence in frame with the active proteins they encode.

Research output: Contribution to journalArticle

Author(s)

SR Heras, MC Thomas, M Garcia, P de Felipe, JL Garcia-Perez, Martin Denis Ryan, MC Lopez

School/Research organisations

Abstract

A comparative analysis of 40 Trypanosoma cruzi L1Tc elements showed that the 2A self-cleaving sequence described in viruses is present in them. Of these elements, 72% maintain the canonical 2A motif (DxExNPGP). A high percentage has a conserved point mutation within the motif that has not been previously described. In vitro and in vivo expression of reporter polyproteins showed that the L1Tc2A sequence is functional. Mutations within certain L1Tc2A sequences affect the efficiency of the cleavage. The data indicate that the L1Tc2A sequence may be influencing the L1Tc enzymatic machinery determining the composition and level of the translated products. The residues located immediately upstream of the 2A consensus sequence increase the cleaving efficiency and appear to stabilize the relative amount of translated products.

Close

Details

Original languageEnglish
Pages (from-to)1449-1460
Number of pages12
JournalCellular and Molecular Life Sciences
Volume63
DOIs
Publication statusPublished - Jun 2006

    Research areas

  • self-cleaving 2A sequence, L1Tc, LINE, retrotransposon, Trypanosoma cruzi, translational regulation, picornavirus, MOUTH-DISEASE VIRUS, OPEN READING FRAME-1, CHAGAS-DISEASE, EXPRESSION, CLEAVAGE, ELEMENTS, GENOME, RNA, LEISHMANIA, MECHANISM

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Therapeutic applications of the 'NPGP' family of viral 2As

    Luke, G. A. & Ryan, M. D., Nov 2018, In : Reviews in Medical Virology. 28, 6, 12 p., e2001.

    Research output: Contribution to journalReview article

  2. Problems in FMD eradication: a way forward?

    Tulloch, F., Luke, G. A. & Ryan, M. D., 5 Jul 2018, In : Journal of Veterinary Medicine and Research. 5, 5, 6 p., 1140.

    Research output: Contribution to journalReview article

  3. Using the 2A Protein Coexpression System: Multicistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins

    Luke, G. A. & Ryan, M. D., 2018, Reporter Gene Assays: Methods and Protocols. Damoiseaux, R. & Hasson, S. (eds.). New York: Humana Press/Springer, Vol. 1755. p. 31-48

    Research output: Chapter in Book/Report/Conference proceedingChapter

  4. The potential consequences for cell Signaling by a class of NOD-Like Receptor proteins (NLRs) bearing an N-terminal signal sequence

    Ryan, M. D., Roulston, C., de Felipe, P., Odon, V., Tilsner, J. & Luke, G. A., 19 May 2017, In : Journal of Cell Signaling. 2, 2, 3 p., 148.

    Research output: Contribution to journalArticle

  5. Foot-and-mouth Disease Virus Proteinases and Polyprotein Processing

    Tulloch, F., Luke, G. A. & Ryan, M. D., 2017, (Accepted/In press) Foot-and-Mouth Disease Virus: Current Research and Emerging Trends. Caister Academic Press, p. 43-60

    Research output: Chapter in Book/Report/Conference proceedingChapter

Related by journal

  1. Regulation of the cardiac sodium pump

    Fuller, W., Tulloch, L., Shattock, M. J., Calaghan, S. C., Howie, J. & Wypijewski, K. J., Apr 2013, In : Cellular and Molecular Life Sciences. 70, 8, p. 1357-1380 24 p.

    Research output: Contribution to journalReview article

  2. The multi-targeted kinase inhibitor sorafenib inhibits human cytomegalovirus replication

    Michaelis, M., Paulus, C., Löschmann, N., Dauth, S., Stange, E., Doerr, H. W., Nevels, M. & Cinatl, J., Mar 2011, In : Cellular and Molecular Life Sciences. 68, 6, p. 1079-90 12 p.

    Research output: Contribution to journalArticle

  3. Epimerases: structure and function

    Allard, STM., Giraud, MF. & Naismith, J. H., Oct 2001, In : Cellular and Molecular Life Sciences. 58, p. 1650-1665 16 p.

    Research output: Contribution to journalArticle

ID: 350965

Top