Skip to content

Research at St Andrews

Label-free optical vibrational spectroscopy to detect the metabolic state of M. tuberculosis cells at the site of disease

Research output: Contribution to journalArticle

Abstract

Tuberculosis relapse is a barrier to shorter treatment. It is thought that lipid rich cells, phenotypically resistant to antibiotics, may play a major role. Most studies investigating relapse use sputum samples although tissue bacteria may play an important role. We developed a nondestructive, label-free technique combining wavelength modulated Raman (WMR) spectroscopy and fluorescence detection (Nile Red staining) to interrogate Mycobacterium tuberculosis cell state. This approach could differentiate single “dormant” (lipid rich, LR) and “non-dormant” (lipid poor, LP) cells with high sensitivity and specificity. We applied this to experimentally infected guinea pig lung sections and were able to distinguish both cell types showing that the LR phenotype dominates in infected tissue. Both in-vitro and ex-vivo spectra correlated well, showing for the first time that Mycobacterium tuberculosis, likely to be phenotypically resistant to antibiotics, are present in large numbers in tissue. This is an important step in understanding the pathology of relapse supporting the idea that they may be caused by M. tuberculosis cells with lipid inclusions.
Close

Details

Original languageEnglish
Article number9844
Number of pages9
JournalScientific Reports
Volume7
DOIs
StatePublished - 29 Aug 2017

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

    Baron, V. O., Chen, M., Clark, S. O., Williams, A., Dholakia, K. & Gillespie, S. H. 2018 Antibiotic Resistance Protocols. Gillespie, S. (ed.). New York, NY: Humana Press/Springer, p. 41-50 10 p. (Methods in Molecular Biology; vol. 1736)

    Research output: Chapter in Book/Report/Conference proceedingChapter

  2. Spatially offset optical coherence tomography: enhancing image contrast at depth

    Chen, M., Wijesinghe, P., Mas Soler, J., Andersen, P. E. & Dholakia, K. 20 Nov 2018 (Submitted) In : Nature Photonics.

    Research output: Contribution to journalArticle

  3. Wide-field multiphoton imaging through scattering media without correction

    Escobet-Montalbán, A., Spesyvtsev, R., Chen, M., Saber, W. A., Andrews, M., Herrington, C. S., Mazilu, M. & Dholakia, K. 12 Oct 2018 In : Science Advances. 4, 10, eaau1338

    Research output: Contribution to journalArticle

  4. Optical trapping with planar silicon metalenses

    Tkachenko, G., Stellinga, D., Ruskuc, A., Chen, M., Dholakia, K. & Krauss, T. F. 15 Jul 2018 In : Optics Letters. 43, 14, p. 3224-3227

    Research output: Contribution to journalArticle

  5. Towards automated cancer screening: label-free classification of fixed cell samples using wavelength modulated Raman spectroscopy

    Woolford, L., Chen, M., Dholakia, K. & Herrington, C. S. Apr 2018 In : Journal of Biophotonics. 11, 4, e201700244

    Research output: Contribution to journalArticle

Related by journal

  1. Scientific Reports (Journal)

    Arso Civil, M. (Reviewer)
    Jul 2018 → …

    Activity: Publication peer-review and editorial workPeer review of manuscripts

  2. Scientific Reports (Journal)

    Borst, A. M. (Reviewer)
    10 Feb 2017

    Activity: Publication peer-review and editorial workPeer review of manuscripts

  3. Scientific Reports (Journal)

    Carroll, E. L. (Member of editorial board)
    3 Oct 2016

    Activity: Publication peer-review and editorial workEditor of research journal

  4. Scientific Reports (Journal)

    Schwarz-Linek, U. (Reviewer)
    11 May 2016

    Activity: Publication peer-review and editorial workPeer review of manuscripts

  5. Scientific Reports (Journal)

    Somorjai, I. M. L. (Reviewer)
    2016 → …

    Activity: Publication peer-review and editorial workPeer review of manuscripts

Related by journal

  1. A large explosive silicic eruption in the British Palaeogene Igneous Province

    Troll, V. R., Emeleus, C. H., Nicoll, G. R., Mattsson, T., Ellam, R. M., Donaldson, C. H. & Harris, C. 24 Jan 2019 In : Scientific Reports. 9, 1, 15 p., 494

    Research output: Contribution to journalArticle

  2. Quantum-dot micropillar lasers subject to coherent time-delayed optical feedback from a short external cavity

    Holzinger, S., Schneider, C., Höfling, S., Porte, X. & Reitzenstein, S. 24 Jan 2019 In : Scientific Reports. 9, 8 p., 631

    Research output: Contribution to journalArticle

  3. Studies of multiferroic palladium perovskites

    Pradhan, D. K., Mishra, A. K., Kumari, S., Basu, A., Somayazulu, M., Gradauskaite, E., Smith, R. M., Gardner, J., Turner, P. W., N’Diaye, A. T., Holcomb, M. B., Katiyar, R. S., Zhou, P., Srinivasan, G., Gregg, J. M. & Scott, J. F. 8 Feb 2019 In : Scientific Reports. 9, 12 p., 1685

    Research output: Contribution to journalArticle

  4. 150,000-year palaeoclimate record from northern Ethiopia supports early, multiple dispersals of modern humans from Africa

    Lamb, H., Bates, C. R., Bryant, C., Davies, S., Huws, D., Marshall, M. & Roberts, H. 18 Jan 2018 In : Scientific Reports. 8, 7 p., 1077

    Research output: Contribution to journalArticle

  5. 3D laser nano-printing on fibre paves the way for super-focusing of multimode laser radiation

    Sokolovskii, G. S., Melissinaki, V., Fedorova, K. A., Dudelev, V., Losev, S. N., Bougrov, V. E., Sibbett, W., Farsari, M. & Rafailov, E. U. 2 Oct 2018 In : Scientific Reports. 8, 9 p., 14618

    Research output: Contribution to journalArticle

ID: 250675702