Skip to content

Research at St Andrews

Label-free optical vibrational spectroscopy to detect the metabolic state of M. tuberculosis cells at the site of disease

Research output: Contribution to journalArticle

Abstract

Tuberculosis relapse is a barrier to shorter treatment. It is thought that lipid rich cells, phenotypically resistant to antibiotics, may play a major role. Most studies investigating relapse use sputum samples although tissue bacteria may play an important role. We developed a nondestructive, label-free technique combining wavelength modulated Raman (WMR) spectroscopy and fluorescence detection (Nile Red staining) to interrogate Mycobacterium tuberculosis cell state. This approach could differentiate single “dormant” (lipid rich, LR) and “non-dormant” (lipid poor, LP) cells with high sensitivity and specificity. We applied this to experimentally infected guinea pig lung sections and were able to distinguish both cell types showing that the LR phenotype dominates in infected tissue. Both in-vitro and ex-vivo spectra correlated well, showing for the first time that Mycobacterium tuberculosis, likely to be phenotypically resistant to antibiotics, are present in large numbers in tissue. This is an important step in understanding the pathology of relapse supporting the idea that they may be caused by M. tuberculosis cells with lipid inclusions.
Close

Details

Original languageEnglish
Article number9844
Number of pages9
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 29 Aug 2017

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

    Baron, V. O., Chen, M., Clark, S. O., Williams, A., Dholakia, K. & Gillespie, S. H., 2018, Antibiotic Resistance Protocols. Gillespie, S. (ed.). New York, NY: Humana Press/Springer, p. 41-50 10 p. (Methods in Molecular Biology; vol. 1736).

    Research output: Chapter in Book/Report/Conference proceedingChapter

  2. Label-free optical hemogram of granulocytes enhanced by artificial neural networks

    Gupta, R., Chen, M., Malcolm, G. P. A., Hempler, N., Dholakia, K. & Powis, S. J., 13 May 2019, In : Optics Express. 27, 10, p. 13706-13720 15 p.

    Research output: Contribution to journalArticle

  3. Overcoming the speckle correlation limit to achieve a fiber wavemeter with attometer resolution

    Bruce, G. D., O'Donnell, L., Chen, M. & Dholakia, K., 15 Mar 2019, In : Optics Letters. 44, 6, p. 1367-1370

    Research output: Contribution to journalArticle

  4. Wide-field multiphoton imaging with TRAFIX

    Escobet-Montalbán, A., Wijesinghe, P., Chen, M. & Dholakia, K., 22 Feb 2019, Multiphoton Microscopy in the Biomedical Sciences XIX. Periasamy, A., So, P. T. C. & König, K. (eds.). Society of Photo-Optical Instrumentation Engineers, p. 49 9 p. 10882G. (Proceedings of SPIE; vol. 10882).

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

  5. Spatially offset optical coherence tomography: enhancing image contrast at depth

    Chen, M., Wijesinghe, P., Mas Soler, J., Andersen, P. E. & Dholakia, K., 20 Nov 2018, (Submitted) In : Nature Photonics.

    Research output: Contribution to journalArticle

Related by journal

  1. Scientific Reports (Journal)

    Alan J. Stewart (Member of editorial board)
    Jun 2019 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  2. Scientific Reports (Journal)

    Monica Arso Civil (Reviewer)
    Jul 2018 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  3. Scientific Reports (Journal)

    Anouk Margaretha Borst (Reviewer)
    10 Feb 2017

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  4. Scientific Reports (Journal)

    Emma Louise Carroll (Member of editorial board)
    3 Oct 2016

    Activity: Publication peer-review and editorial work typesEditor of research journal

  5. Scientific Reports (Journal)

    Uli Schwarz-Linek (Reviewer)
    11 May 2016

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. A comparative analysis of response times shows that multisensory benefits and interactions are not equivalent

    Innes, B. R. & Otto, T. U., 27 Feb 2019, In : Scientific Reports. 9, 10 p., 2921.

    Research output: Contribution to journalArticle

  2. A large explosive silicic eruption in the British Palaeogene Igneous Province

    Troll, V. R., Emeleus, C. H., Nicoll, G. R., Mattsson, T., Ellam, R. M., Donaldson, C. H. & Harris, C., 24 Jan 2019, In : Scientific Reports. 9, 1, 15 p., 494.

    Research output: Contribution to journalArticle

  3. Automated tumour budding quantification by machine learning augments TNM staging in muscle-invasive bladder cancer prognosis

    Brieu, N., Gavriel, C., Nearchou, I. P., Harrison, D. J., Schmidt, G. & Caie, P. D., 26 Mar 2019, In : Scientific Reports. 9, 5174.

    Research output: Contribution to journalArticle

  4. Classifying grey seal behaviour in relation to environmental variability and commercial fishing activity - a multivariate hidden Markov model

    van Beest, F. M., Mews, S., Elkenkamp, S., Schuhmann, P., Tsolak, D., Wobbe, T., Bartolino, V., Bastardie, F., Dietz, R., von Dorrien, C., Galatius, A., Karlsson, O., McConnell, B., Nabe-Nielsen, J., Olsen, M. T., Teilmann, J. & Langrock, R., 4 Apr 2019, In : Scientific Reports. 9, 14 p., 5642.

    Research output: Contribution to journalArticle

  5. Defining the genes for the final steps in biosynthesis of the complex polyketide antibiotic mupirocin by Pseudomonas fluorescens NCIMB10586

    Connolly, J. A., Wilson, A., Macioszek, M., Song, Z., Wang, L., Mohammad, H. H., Yadav, M., di Martino, M., Miller, C. E., Hothersall, J., Haines, A. S., Stephens, E. R., Crump, M. P., Willis, C. L., Simpson, T. J., Winn, P. J. & Thomas, C. M., 7 Feb 2019, In : Scientific Reports. 9, 9 p., 1542.

    Research output: Contribution to journalArticle

ID: 250675702