Skip to content

Research at St Andrews

Light-induced cell separation in a tailored optical landscape

Research output: Contribution to journalArticlepeer-review

DOI

Author(s)

L Paterson, E Papagiakoumou, Graham Frank Milne, V Garces-Chavez, S A Tatarkova, W Sibbett, F J Gunn-Moore, P E Bryant, A C Riches, K Dholakia

School/Research organisations

Abstract

We demonstrate passive optical sorting of cell populations in the absence of any externally driven fluid flow. Specifically, we report the movement of erythrocytes and lymphocytes in an optical landscape, consisting of a circularly symmetric light pattern created by a Bessel light beam. These distinct cell populations move, spontaneously and differentially, across the underlying periodic optical landscape. Thus, we were able to separate lymphocytes from a mixed population of cells containing erythrocytes and then collect the lymphocytes in a microcapillary reservoir. We also demonstrate an enhanced form of this separation that exploits the polarizability of silica microspheres by attaching spheres coated with antibodies to cell surface markers to a subpopulation of lymphocytes. These techniques may be applied using standard laboratory apparatus. (c) 2005 American Institute of Physics.

Close

Details

Original languageEnglish
Pages (from-to)123901
Number of pages3
JournalApplied Physics Letters
Volume87
Issue number12
DOIs
Publication statusPublished - 19 Sep 2005

    Research areas

  • PARTICLES, FORCES, TRAP, BEAM

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Femtosecond optical transfection of cells: viability and efficiency

    Stevenson, D., Agate, B., Tsampoula, X., Fischer, P., Brown, C. T. A., Sibbett, W., Riches, A. C., Gunn-Moore, F. J. & Dholakia, K., 7 Aug 2006, In: Optics Express. 14, 16, p. 7125-7133 9 p.

    Research output: Contribution to journalArticlepeer-review

  2. Optical transfection of mammalian cells - art. no. 61910D

    Stevenson, D. J., Agate, B., Paterson, L., Lake, T., Comrie, M., Brown, T., Riches, A., Bryant, P., Sibbett, W., Gunn-Moore, F. & Dholakia, K., 2006, Biophotonics and new therapy frontiers . Grzymala, R. & Haeberle, O. (eds.). SPIE, p. 1910-1910 8 p. (Proceeding of SPIE - The International Society for Optical Engineering; vol. 6191).

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

  3. Violet diode-assisted photoporation and transfection of cells

    Paterson, L., Agate, B., Comrie, M., Ferguson, R., Stevenson, D., Lake, T., Brown, C., Gunn-Moore, F. J., Riches, A. C., Bryant, P. E., Sibbett, W. & Dholakia, K., Aug 2005

    Research output: Other contribution

  4. Photoporation and cell transfection using a violet diode laser

    Paterson, L., Agate, B., Comrie, M., Ferguson, R., Lake, TK., Morris, JE., Carruthers, AE., Brown, C. T. A., Sibbett, W., Bryant, P. E., Gunn-Moore, F. J., Riches, A. C. & Dholakia, K., 24 Jan 2005, In: Optics Express. 13, 2, p. 595-600 6 p.

    Research output: Contribution to journalArticlepeer-review

  5. Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells

    Mthunzi, P., Lee, W. M., Riches, A. C., Brown, C. T. A., Gunn-Moore, F. J. & Dholakia, K., 2010, In: IEEE Journal of Selected Topics in Quantum Electronics. 16, 3, p. 608-618 11 p.

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. Applied Physics Letters (Journal)

    Finlay Morrison (Reviewer)

    2008 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. Continuous-wave operation of vertically emitting ring interband cascade lasers at room temperature

    Knoetig, H., Hinkov, B., Weih, R., Hoefling, S., Koeth, J. & Strasser, G., 30 Mar 2020, In: Applied Physics Letters. 116, 13, 5 p., 131101.

    Research output: Contribution to journalArticlepeer-review

  2. Nanodomain patterns in ultra-tetragonal lead titanate (PbTiO3)

    Kumar, A., Guy, J. G. M., Zhang, L., Chen, J., Gregg, J. M. & Scott, J. F., 5 May 2020, In: Applied Physics Letters. 116, 18, 5 p., 182903.

    Research output: Contribution to journalArticlepeer-review

  3. Photonics based perfect secrecy cryptography: toward fully classical implementations

    Mazzone, V., Di Falco, A., Cruz, A. & Fratalocchi, A., 29 Jun 2020, In: Applied Physics Letters. 116, 26, 5 p., 260502.

    Research output: Contribution to journalArticlepeer-review

  4. Spatio-temporal coherence in vertically emitting GaAs-based electrically driven polariton lasers

    Suchomel, H., Klaas, M., Betzold, S., Gagel, P., Beierlein, J., Klembt, S., Schneider, C. & Höfling, S., 27 Apr 2020, In: Applied Physics Letters. 116, 17, 171103.

    Research output: Contribution to journalArticlepeer-review

  5. Atomic ordering and bond relaxation in optical spectra of self-organized InP/GaInP2 Wigner molecule structures

    Mintairov, A. M., Lebedev, D. V., Bert, N., Belyaev, K. G., Nevedomskiy, V. N., Rakhlin, M. V., Toropov, A. A., Vlasov, A. S., Gocalinska, A., Juska, G., Pelucchi, E., Arredondo, M. A., Naden, A. B., Shelaev, A. V. & Bykov, V. A., 12 Nov 2019, In: Applied Physics Letters. 115, 20, 5 p., 202104.

    Research output: Contribution to journalArticlepeer-review

ID: 696834

Top