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Light-sheet microscopy using an Airy beam

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Abstract

Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.
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Original languageEnglish
Pages (from-to)541-544
Number of pages7
JournalNature Methods
Volume11
Issue number5
Early online date6 Apr 2014
DOIs
StatePublished - May 2014

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