Skip to content

Research at St Andrews

Light-sheet microscopy with attenuation-compensated propagation-invariant beams

Research output: Contribution to journalArticle

Abstract

Scattering and absorption limit the penetration of optical fields into tissue. We demonstrate a new approach for increased depth penetration in light-sheet microscopy: attenuation-compensation of the light field. This tailors an exponential intensity increase along the illuminating propagation-invariant field, enabling the redistribution of intensity strategically within a sample to maximize signal and minimize irradiation. A key attribute of this method is that only minimal knowledge of the specimen transmission properties is required. We numerically quantify the imaging capabilities of attenuation-compensated Airy and Bessel light sheets, showing that increased depth penetration is gained without compromising any other beam attributes. This powerful yet straightforward concept, combined with the self-healing properties of the propagation-invariant field, improves the contrast-to-noise ratio of light-sheet microscopy up to eightfold across the entire field of view in thick biological specimens. This improvement can significantly increase the imaging capabilities of light-sheet microscopy techniques using Airy, Bessel, and other propagation-invariant beam types, paving the way for widespread uptake by the biomedical community.
Close

Details

Original languageEnglish
Article numbereaar4817
Number of pages14
JournalScience Advances
Volume4
Issue number4
DOIs
StatePublished - 6 Apr 2018

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

    Nylk, J., Kristensen, M. V. G., Mazilu, M., Kubasik-Thayil, A., Mitchell, C. A., Campbell, E. C., Powis, S. J., Gunn-Moore, F. J. & Dholakia, K. 1 Apr 2015 In : Biomedical Optics Express. 6, 4, p. 1512-1519

    Research output: Contribution to journalArticle

  2. A compact Airy beam light sheet microscope with a tilted cylindrical lens

    Yang, Z., Prokopas, M., Nylk, J., Coll Llado, C., Gunn-Moore, F. J., Ferrier, D. E. K., Vettenburg, T. & Dholakia, K. 1 Oct 2014 In : Biomedical Optics Express. 5, 10, p. 3434-3442

    Research output: Contribution to journalArticle

  3. Light-sheet microscopy using an Airy beam

    Vettenburg, T., Dalgarno, H. I. C., Nylk, J., Coll-Lladó, C., Ferrier, D. E. K., Cizmar, T., Gunn-Moore, F. J. & Dholakia, K. May 2014 In : Nature Methods. 11, 5, p. 541-544 7 p.

    Research output: Contribution to journalArticle

  4. Multimode fibre based imaging for optically cleared samples

    Gusachenko, I., Nylk, J., Tello, J. A. & Dholakia, K. 1 Nov 2017 In : Biomedical Optics Express. 8, 11, p. 5179-5190

    Research output: Contribution to journalArticle

  5. Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue

    Nylk, J., McCluskey, K. A., Aggarwal, S., Tello, J. & Dholakia, K. 1 Oct 2016 In : Biomedical Optics Express. 7, 10, p. 4021-4033

    Research output: Contribution to journalArticle

Related by journal

  1. The comparative genomics and complex population history of Papio baboons

    Rogers, J. , Raveendran, M. , Harris, R. A. , Mailund, T. , Leppälä, K. , Athanasiadis, G. , Schierup, M. H. , Cheng, J. , Munch, K. , Walker, J. A. , Konkel, M. K. , Jordan, V. , Steely, C. J. , Beckstrom, T. O. , Bergey, C. , Burrell, A. , Schrempf, D. , Noll, A. , Kothe, M. , Kopp, G. H. & 22 others Liu, Y., Murali, S., Billis, K., Martin, F. J., Muffato, M., Cox, L., Else, J., Disotell, T., Muzny, D. M., Phillips-conroy, J., Aken, B., Eichler, E. E., Marques-bonet, T., Kosiol, C., Batzer, M. A., Hahn, M. W., Tung, J., Zinner, D., Roos, C., Jolly, C. J., Gibbs, R. A. & Worley, K. C. 30 Jan 2019 In : Science Advances. 5, 1, 15 p., eaau6947

    Research output: Contribution to journalArticle

  2. A molecular mechanism for the enzymatic methylation of nitrogen atoms within peptide bonds

    Song, H., Van Der Velden, N. S., Shirran, S. L., Bleiziffer, P., Zach, C., Sieber, R., Imani, A. S., Krausbeck, F., Aebi, M., Freeman, M. F., Riniker, S., Künzler, M. & Naismith, J. H. 24 Aug 2018 In : Science Advances. 4, 8, 13 p., eaat2720

    Research output: Contribution to journalArticle

  3. Chronology of martian breccia NWA 7034 and the formation of the martian crustal dichotomy

    Cassata, W. S., Cohen, B. E., Mark, D. F., Trappitsch, R., Crow, C. A., Wimpenny, J., Lee, M. R. & Smith, C. L. 23 May 2018 In : Science Advances. 4, 5, 12 p., eaap8306

    Research output: Contribution to journalArticle

  4. Coherent order parameter oscillations in the ground state of the excitonic insulator Ta2NiSe5

    Werdehausen, D., Takayama, T., Höppner, M., Albrecht, G., Rost, A. W., Lu, Y., Manske, D., Takagi, H. & Kaiser, S. 23 Mar 2018 In : Science Advances. 4, 3, 7 p., eaap8652

    Research output: Contribution to journalArticle

  5. Genes for de novo biosynthesis of omega-3 polyunsaturated fatty acids are widespread in animals

    Kabeya, N., Fonseca, M., Ferrier, D. E. K., Navarro, J., Bay, L., Francis, D., Tocher, D., Castro, L. F. C. & Monroig, Ó. 2 May 2018 In : Science Advances. 4, 5, 9 p., eaar6849

    Research output: Contribution to journalArticle

ID: 252130861