Skip to content

Research at St Andrews

Linear Eyring plots conceal a change in rate-limiting step in an enzyme reaction

Research output: Contribution to journalArticle

Author(s)

Teresa Filipa Guerreiro Machado, Tracey Gloster, Rafael Guimaraes da Silva

School/Research organisations

Abstract

The temperature dependence of psychrophilic and mesophilic (R)-3-hydroxybutyrate dehydrogenase steady-state rates yields nonlinear and linear Eyring plots, respectively. Solvent viscosity effects and multiple- and single-turnover pre-steady-state kinetics demonstrate that while product release is rate-limiting at high temperatures for the psychrophilic enzyme, either interconversion between enzyme-substrates and enzyme-products complexes or a step prior to it limits the rate at low temperature. Unexpectedly, a similar change in rate-limiting step is observed with the mesophilic en-zyme, where a step prior to chemistry becomes rate-limiting at low temperature. This observation may have implica-tions for past and future interpretation of temperature-rate profiles.
Close

Details

Original languageEnglish
Pages (from-to)6757-6761
JournalBiochemistry
Volume57
Issue number49
Early online date26 Nov 2018
DOIs
Publication statusPublished - 11 Dec 2018

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. New irreversible α-ʟ-iduronidase inhibitors and activity-based probes

    Artola, M., Kuo, C-L., McMahon, S. A., Oehler, V., Hansen, T., van der Lienden, M., He, X., van den Elst, H., Florea, B. I., Kermode, A. R., van der Marel, G. A., Gloster, T. M., Codée, J. D. C., Overkleeft, H. S. & Aerts, J. M. F. G., 26 Nov 2018, In : Chemistry - A European Journal. Early View, 9 p.

    Research output: Contribution to journalArticle

  2. Revealing the mechanism for covalent inhibition of glycoside hydrolases by carbasugars at an atomic level

    Ren, W., Pengelly, R. J., Farren-Dai, M., Abadi, S. S. K., Oehler, V., Akintola, O., Draper, J., Meanwell, M., Chakladar, S., Swiderek, K., Moliner, V., Britton, R., Gloster, T. & Bennet, A., 13 Aug 2018, In : Nature Communications. 9, 12 p., 3243.

    Research output: Contribution to journalArticle

  3. Allosteric activation shifts the rate-limiting step in a short-form ATP phosphoribosyltransferase

    Fisher, G., Thomson, C. M., Stroek, R., Czekster, C. M., Hirschi, J. S. & da Silva, R. G., 24 Jul 2018, In : Biochemistry. 57, 29, p. 4357-4367

    Research output: Contribution to journalArticle

  4. Metabolic inhibitors of O-GlcNAc transferase (OGT) that act in vivo implicate decreased O-GlcNAc levels in leptin-mediated nutrient sensing

    Liu, T., Zandberg, W. F., Gloster, T. M., Deng, L., Murray, K. D., Shan, X. & Vocadlo, D. J., 25 Jun 2018, In : Angewandte Chemie International Edition. 57, 26, p. 7644-7648

    Research output: Contribution to journalArticle

  5. Catalytic and anticatalytic snapshots of a short-form ATP phosphoribosyltransferase

    Alphey, M. S., Fisher, G., Ge, Y., Gould, E. R., Guerreiro Machado, T. F., Liu, H., Florence, G. J., Naismith, J. H. & da Silva, R. G., 11 May 2018, In : ACS Catalysis. 8, p. 5601-5610 10 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Insights into the mechanism of the cyanobactin heterocyclase enzyme

    Ge, Y., Czekster, C. M., Miller, O. K., Botting, C. H., Schwarz-Linek, U. & Naismith, J. H., 23 Apr 2019, In : Biochemistry. 58, 16, p. 2125-2132 8 p.

    Research output: Contribution to journalArticle

  2. Allosteric activation shifts the rate-limiting step in a short-form ATP phosphoribosyltransferase

    Fisher, G., Thomson, C. M., Stroek, R., Czekster, C. M., Hirschi, J. S. & da Silva, R. G., 24 Jul 2018, In : Biochemistry. 57, 29, p. 4357-4367

    Research output: Contribution to journalArticle

  3. Oxidation of the cyanobactin precursor peptide is independent of the leader peptide and operates in a defined order

    Gao, S., Ge, Y., Bent, A. F., Schwarz-Linek, U. & Naismith, J. H., 16 Oct 2018, In : Biochemistry. 57, 41, p. 5996-6002 7 p.

    Research output: Contribution to journalArticle

  4. Adenosine monophosphate binding stabilizes the KTN domain of the Shewanella denitrificans Kef potassium efflux system

    Pliotas, C., Grayer, S. C., Ekkerman, S., Chan, A. K. N., Healy, J., Marius, P., Bartlett, W., Khan, A., Cortopassi, W. A., Chandler, S. A., Rasmussen, T., Benesch, J. L. P., Paton, R. S., Claridge, T. D. W., Miller, S., Booth, I. R., Naismith, J. H. & Conway, S. J., 15 Aug 2017, In : Biochemistry. 56, 32, p. 4219-4234

    Research output: Contribution to journalArticle

ID: 256704869