Skip to content

Research at St Andrews

Long-term imaging of cellular forces with high precision by elastic resonator interference stress microscopy

Research output: Contribution to journalArticle

DOI

Open Access permissions

Open

Author(s)

Nils M. Kronenberg, Philipp Liehm, Anja Steude, Johanna A. Knipper, Jessica G. Borger, Giuliano Scarcelli, Kristian Franze, Simon J. Powis, Malte C. Gather

School/Research organisations

Abstract

Cellular forces are crucial for many biological processes but current methods to image them have limitations with respect to data analysis, resolution and throughput. Here, we present a robust approach to measure mechanical cell–substrate interactions in diverse biological systems by interferometrically detecting deformations of an elastic micro-cavity. Elastic resonator interference stress microscopy (ERISM) yields stress maps with exceptional precision and large dynamic range (2 nm displacement resolution over a >1 μm range, translating into 1 pN force sensitivity). This enables investigation of minute vertical stresses (<1 Pa) involved in podosome protrusion, protein-specific cell–substrate interaction and amoeboid migration through spatial confinement in real time. ERISM requires no zero-force reference and avoids phototoxic effects, which facilitates force monitoring over multiple days and at high frame rates and eliminates the need to detach cells after measurements. This allows observation of slow processes such as differentiation and further investigation of cells, for example, by immunostaining.
Close

Details

Original languageEnglish
Pages (from-to)864-872
JournalNature Cell Biology
Volume19
Issue number7
Early online date19 Jun 2017
DOIs
Publication statusPublished - Jul 2017

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Non-obstructive intracellular nanolasers

    Fikouras, A., Schubert, M., Karl, M., Kumar, J. D., Powis, S. J., Di Falco, A. & Gather, M. C., 16 Nov 2018, In : Nature Communications. 9, 7 p., 4817.

    Research output: Contribution to journalLetter

  2. Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators

    Schubert, M., Volckaert, K., Karl, M., Morton, A., Liehm, P., Miles, G. B., Powis, S. J. & Gather, M. C., 19 Jan 2017, In : Scientific Reports. 7, 40877.

    Research output: Contribution to journalArticle

  3. Lasing within live cells containing intracellular optical micro-resonators for barcode-type cell tagging and tracking

    Schubert, M., Steude, A., Liehm, P., Kronenberg, N. M., Karl, M., Campbell, E. C., Powis, S. J. & Gather, M. C., 12 Aug 2015, In : Nano Letters. 15, 8, p. 5674-5652 6 p.

    Research output: Contribution to journalArticle

  4. Narrowband organic light-emitting diodes for fluorescence microscopy and calcium imaging

    Murawski, C., Mischok, A., Booth, J. H., Kumar, J. D., Archer, E., Tropf, L. C., Keum, C., Deng, Y., Yoshida, K., Samuel, I. D. W., Schubert, M., Pulver, S. & Gather, M. C., 5 Sep 2019, In : Advanced Materials. Early View, 8 p.

    Research output: Contribution to journalArticle

  5. Low-threshold polariton lasing in a highly disordered conjugated polymer

    Wei, M., Rajendran, S. K., Ohadi, H., Tropf, L. C., Gather, M. C., Turnbull, G. A. & Samuel, I. D. W., 27 Aug 2019, In : Optica. 6, 9, p. 1124-1129 6 p., 362590.

    Research output: Contribution to journalArticle

Related by journal

  1. Cytonemes are required for the establishment of a normal Hedgehog morphogen gradient in Drosophila epithelia

    Bischoff, M., Gradilla, A-C., Seijo, I., Andres, G., Rodriguez-Navas, C., Gonzalez-Mendez, L. & Guerrero, I., 2013, In : Nature Cell Biology. 15, p. 1269-1281

    Research output: Contribution to journalArticle

  2. The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast

    Ferreira, H. C., Luke, B., Schober, H., Kalck, V., Lingner, J. & Gasser, S. M., Jul 2011, In : Nature Cell Biology. 13, 7, p. 867-874

    Research output: Contribution to journalArticle

  3. Induction of autophagy by spermidine promotes longevity

    Eisenberg, T., Knauer, H., Schauer, A., Buttner, S., Ruckenstuhl, C., Carmona-Gutierrez, D., Ring, J., Schroeder, S., Magnes, C., Antonacci, L., Fussi, H., Deszcz, L., Hartl, R., Schraml, E., Criollo, A., Megalou, E., Weiskopf, D., Laun, P., Heeren, G., Breitenbach, M. & 9 others, Grubeck-Loebenstein, B., Herker, E., Fahrenkrog, B., Frohlich, KU., Sinner, F., Tavernarakis, N., Minois, N. B., Kroemer, G. & Madeo, F., 2009, In : Nature Cell Biology. 11, 11, p. 1305-1314

    Research output: Contribution to journalArticle

  4. FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P

    Godi, A., Di Campli, A., Konstantakopoulos, A., Di Tullio, G., Alessi, D. R., Kular, G. S., Daniele, T., Marra, P., Lucocq, J. M. & De Matteis, M. A., May 2004, In : Nature Cell Biology. 6, 5, p. 393-404 12 p.

    Research output: Contribution to journalArticle

ID: 249941513

Top