Skip to content

Research at St Andrews

Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry

Research output: Contribution to journalArticle

DOI

Author(s)

Richard J A Goodwin, Andrew R Pitt, David Harrison, Stefan K Weidt, Pat R R Langridge-Smith, Michael P Barrett, C Logan Mackay

School/Research organisations

Abstract

Mass spectrometry imaging (MSI) is a powerful tool in metabolomics and proteomics for the spatial localization and identification of pharmaceuticals, metabolites, lipids, peptides and proteins in biological tissues. However, sample preparation remains a crucial variable in obtaining the most accurate distributions. Common washing steps used to remove salts, and solvent-based matrix application, allow analyte spreading to occur. Solvent-free matrix applications can reduce this risk, but increase the possibility of ionisation bias due to matrix adhesion to tissue sections. We report here the use of matrix-free MSI using laser desorption ionisation performed on a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. We used unprocessed tissue with no post-processing following thaw-mounting on matrix-assisted laser desorption ionisation (MALDI) indium-tin oxide (ITO) target plates. The identification and distribution of a range of phospholipids in mouse brain and kidney sections are presented and compared with previously published MALDI time-of-flight (TOF) MSI distributions.
Close

Details

Original languageEnglish
Pages (from-to)969-972
Number of pages4
JournalRapid Communications in Mass Spectrometry
Volume25
Issue number7
DOIs
Publication statusPublished - 2011

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The landscape of genomic copy number alterations in colorectal cancer and their consequences on gene expression levels and disease outcome

    Ried, T., Meijer, G. A., Harrison, D. J., Grech, G., Franch-Expósito, S., Briffa, R., Carvalho, B. & Camps, J., 6 Aug 2019, In : Molecular Aspects of Medicine. In press

    Research output: Contribution to journalArticle

  2. Genome-scale CRISPR/Cas9 screen determines factors modulating sensitivity to ProTide NUC-1031

    Sarr, A., Bré, J., Um, I. H., Chan, T. H., Mullen, P., Harrison, D. J. & Reynolds, P. A., 21 May 2019, In : Scientific Reports. 9, 13 p., 7643.

    Research output: Contribution to journalArticle

  3. Raman spectroscopy investigation of biochemical changes in tumor spheroids with aging and after treatment with staurosporine

    Jamieson, L. E., Harrison, D. J. & Campbell, C. J., May 2019, In : Journal of Biophotonics. 12, 5, e201800201.

    Research output: Contribution to journalArticle

Related by journal

  1. Rapid Communications in Mass Spectrometry (Journal)

    Nora Nell Hanson (Reviewer)
    Dec 2011

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. Changes in stable isotope compositions during fasting in phocid seals

    Habran, S., Damseaux, F., Pomeroy, P., Debier, C., Crocker, D., Lepoint, G. & Das, K., 30 Jan 2019, In : Rapid Communications in Mass Spectrometry. 33, 2, p. 176-184 9 p.

    Research output: Contribution to journalArticle

  2. Triple oxygen isotope analysis of nitrate using isotope exchange - cavity ringdown laser spectroscopy

    Gázquez, F. & Claire, M. W., 30 Nov 2018, In : Rapid Communications in Mass Spectrometry. 32, 22, p. 1949-1961

    Research output: Contribution to journalArticle

  3. Recovery of organic matter from mineral-rich sediment and soils for stable isotope analyses using static dense media

    Wurster, C. M., Saiz, G., Calder, A. & Bird, M. I., 15 Jan 2010, In : Rapid Communications in Mass Spectrometry. 24, 1, p. 165-168 4 p.

    Research output: Contribution to journalArticle

ID: 173395369