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Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States

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Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States. / Mohamed, Naglaa; Timofeyeva, Yekaterina; Jamrozy, Dorota; Rojas, Eduardo; Hao, Li; Silmon de Monerri, Natalie C.; Hawkins, Julio; Singh, Guy; Cai, Bing; Liberator, Paul; Sebastian, Shite; Donald, Robert G. K.; Scully, Ingrid L.; Jones, C. Hal; Creech, C. Buddy; Thomsen, Isaac; Parkhill, Julian; Peacock, Sharon J.; Jansen, Kathrin U.; Holden, Matthew T. G.; Anderson, Annaliesa S.

In: PLoS ONE, Vol. 14, No. 1, e0208356, 14.01.2019.

Research output: Contribution to journalArticle

Harvard

Mohamed, N, Timofeyeva, Y, Jamrozy, D, Rojas, E, Hao, L, Silmon de Monerri, NC, Hawkins, J, Singh, G, Cai, B, Liberator, P, Sebastian, S, Donald, RGK, Scully, IL, Jones, CH, Creech, CB, Thomsen, I, Parkhill, J, Peacock, SJ, Jansen, KU, Holden, MTG & Anderson, AS 2019, 'Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States' PLoS ONE, vol. 14, no. 1, e0208356. https://doi.org/10.1371/journal.pone.0208356

APA

Mohamed, N., Timofeyeva, Y., Jamrozy, D., Rojas, E., Hao, L., Silmon de Monerri, N. C., ... Anderson, A. S. (2019). Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States. PLoS ONE, 14(1), [e0208356]. https://doi.org/10.1371/journal.pone.0208356

Vancouver

Mohamed N, Timofeyeva Y, Jamrozy D, Rojas E, Hao L, Silmon de Monerri NC et al. Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States. PLoS ONE. 2019 Jan 14;14(1). e0208356. https://doi.org/10.1371/journal.pone.0208356

Author

Mohamed, Naglaa ; Timofeyeva, Yekaterina ; Jamrozy, Dorota ; Rojas, Eduardo ; Hao, Li ; Silmon de Monerri, Natalie C. ; Hawkins, Julio ; Singh, Guy ; Cai, Bing ; Liberator, Paul ; Sebastian, Shite ; Donald, Robert G. K. ; Scully, Ingrid L. ; Jones, C. Hal ; Creech, C. Buddy ; Thomsen, Isaac ; Parkhill, Julian ; Peacock, Sharon J. ; Jansen, Kathrin U. ; Holden, Matthew T. G. ; Anderson, Annaliesa S. / Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States. In: PLoS ONE. 2019 ; Vol. 14, No. 1.

Bibtex - Download

@article{afd4f50714c345b98d9d082660414933,
title = "Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States",
abstract = "Staphylococcus aureus capsular polysaccharides (CP) are important virulence factors under evaluation as vaccine antigens. Clinical S. aureus isolates have the biosynthetic capability to express either CP5 or CP8 and an understanding of the relationship between CP genotype/phenotype and S. aureus epidemiology is valuable. Using whole genome sequencing, the clonal relatedness and CP genotype were evaluated for disease-associated S. aureus isolates selected from the Tigecycline Evaluation and Surveillance Trial (T.E.S.T) to represent different geographic regions in the United States (US) during 2004 and 2009–10. Thirteen prominent clonal complexes (CC) were identified, with CC5, 8, 30 and 45 representing >80{\%} of disease isolates. CC5 and CC8 isolates were CP type 5 and, CC30 and CC45 isolates were CP type 8. Representative isolates from prevalent CC were susceptible to in vitro opsonophagocytic killing elicited by anti-CP antibodies, demonstrating that susceptibility to opsonic killing is not linked to the genetic lineage. However, as not all S. aureus isolates may express CP, isolates representing the diversity of disease isolates were assessed for CP production. While approximately 35{\%} of isolates (primarily CC8) did not express CP in vitro, CP expression could be clearly demonstrated in vivo for 77{\%} of a subset of these isolates (n = 20) despite the presence of mutations within the capsule operon. CP expression in vivo was also confirmed indirectly by measuring an increase in CP specific antibodies in mice infected with CP5 or CP8 isolates. Detection of antigen expression in vivo in relevant disease states is important to support the inclusion of these antigens in vaccines. Our findings confirm the validity of CP as vaccine targets and the potential of CP-based vaccines to contribute to S. aureus disease prevention.",
author = "Naglaa Mohamed and Yekaterina Timofeyeva and Dorota Jamrozy and Eduardo Rojas and Li Hao and {Silmon de Monerri}, {Natalie C.} and Julio Hawkins and Guy Singh and Bing Cai and Paul Liberator and Shite Sebastian and Donald, {Robert G. K.} and Scully, {Ingrid L.} and Jones, {C. Hal} and Creech, {C. Buddy} and Isaac Thomsen and Julian Parkhill and Peacock, {Sharon J.} and Jansen, {Kathrin U.} and Holden, {Matthew T. G.} and Anderson, {Annaliesa S.}",
year = "2019",
month = "1",
day = "14",
doi = "10.1371/journal.pone.0208356",
language = "English",
volume = "14",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States

AU - Mohamed, Naglaa

AU - Timofeyeva, Yekaterina

AU - Jamrozy, Dorota

AU - Rojas, Eduardo

AU - Hao, Li

AU - Silmon de Monerri, Natalie C.

AU - Hawkins, Julio

AU - Singh, Guy

AU - Cai, Bing

AU - Liberator, Paul

AU - Sebastian, Shite

AU - Donald, Robert G. K.

AU - Scully, Ingrid L.

AU - Jones, C. Hal

AU - Creech, C. Buddy

AU - Thomsen, Isaac

AU - Parkhill, Julian

AU - Peacock, Sharon J.

AU - Jansen, Kathrin U.

AU - Holden, Matthew T. G.

AU - Anderson, Annaliesa S.

PY - 2019/1/14

Y1 - 2019/1/14

N2 - Staphylococcus aureus capsular polysaccharides (CP) are important virulence factors under evaluation as vaccine antigens. Clinical S. aureus isolates have the biosynthetic capability to express either CP5 or CP8 and an understanding of the relationship between CP genotype/phenotype and S. aureus epidemiology is valuable. Using whole genome sequencing, the clonal relatedness and CP genotype were evaluated for disease-associated S. aureus isolates selected from the Tigecycline Evaluation and Surveillance Trial (T.E.S.T) to represent different geographic regions in the United States (US) during 2004 and 2009–10. Thirteen prominent clonal complexes (CC) were identified, with CC5, 8, 30 and 45 representing >80% of disease isolates. CC5 and CC8 isolates were CP type 5 and, CC30 and CC45 isolates were CP type 8. Representative isolates from prevalent CC were susceptible to in vitro opsonophagocytic killing elicited by anti-CP antibodies, demonstrating that susceptibility to opsonic killing is not linked to the genetic lineage. However, as not all S. aureus isolates may express CP, isolates representing the diversity of disease isolates were assessed for CP production. While approximately 35% of isolates (primarily CC8) did not express CP in vitro, CP expression could be clearly demonstrated in vivo for 77% of a subset of these isolates (n = 20) despite the presence of mutations within the capsule operon. CP expression in vivo was also confirmed indirectly by measuring an increase in CP specific antibodies in mice infected with CP5 or CP8 isolates. Detection of antigen expression in vivo in relevant disease states is important to support the inclusion of these antigens in vaccines. Our findings confirm the validity of CP as vaccine targets and the potential of CP-based vaccines to contribute to S. aureus disease prevention.

AB - Staphylococcus aureus capsular polysaccharides (CP) are important virulence factors under evaluation as vaccine antigens. Clinical S. aureus isolates have the biosynthetic capability to express either CP5 or CP8 and an understanding of the relationship between CP genotype/phenotype and S. aureus epidemiology is valuable. Using whole genome sequencing, the clonal relatedness and CP genotype were evaluated for disease-associated S. aureus isolates selected from the Tigecycline Evaluation and Surveillance Trial (T.E.S.T) to represent different geographic regions in the United States (US) during 2004 and 2009–10. Thirteen prominent clonal complexes (CC) were identified, with CC5, 8, 30 and 45 representing >80% of disease isolates. CC5 and CC8 isolates were CP type 5 and, CC30 and CC45 isolates were CP type 8. Representative isolates from prevalent CC were susceptible to in vitro opsonophagocytic killing elicited by anti-CP antibodies, demonstrating that susceptibility to opsonic killing is not linked to the genetic lineage. However, as not all S. aureus isolates may express CP, isolates representing the diversity of disease isolates were assessed for CP production. While approximately 35% of isolates (primarily CC8) did not express CP in vitro, CP expression could be clearly demonstrated in vivo for 77% of a subset of these isolates (n = 20) despite the presence of mutations within the capsule operon. CP expression in vivo was also confirmed indirectly by measuring an increase in CP specific antibodies in mice infected with CP5 or CP8 isolates. Detection of antigen expression in vivo in relevant disease states is important to support the inclusion of these antigens in vaccines. Our findings confirm the validity of CP as vaccine targets and the potential of CP-based vaccines to contribute to S. aureus disease prevention.

U2 - 10.1371/journal.pone.0208356

DO - 10.1371/journal.pone.0208356

M3 - Article

VL - 14

JO - PLoS One

T2 - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 1

M1 - e0208356

ER -

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