Skip to content

Research at St Andrews

Monitoring the Rab27 associated exosome pathway using nanoparticle tracking analysis

Research output: Contribution to journalArticle


Ying Zheng, Elaine C Campbell, John Lucocq, Andrew Riches, Simon J Powis

School/Research organisations


Exosomes are secreted by many cell types and display multiple biological functions. The ability to both rapidly detect and quantify exosomes in biological samples would assist in the screening of agents that interfere with their release, and which may therefore be of clinical relevance. Nanoparticle tracking analysis, which detects the size and concentration of exosomes, was used to monitor the inhibition of exosome secretion from MDA-MB-231 breast cancer cells expressing inhibitory RNA targeted for Rab27a, a known component of the exosome pathway. Inhibition of both Rab27a and Rab27b was observed, resulting in alterations to intracellular CD63+ compartments and the release of fewer exosomes into the culture medium, as determined by nanoparticle tracking analysis and confirmed by immunoblotting and protein quantification. These data show that nanoparticle tracking analysis can be used effectively and rapidly to monitor the disruption of exosome secretion.


Original languageEnglish
Pages (from-to)1706-13
Number of pages8
JournalExperimental Cell Research
Issue number12
Publication statusPublished - 15 Jul 2013

    Research areas

  • Antigens, CD63, Cell Line, Tumor, Exosomes, Humans, Nanoparticles, RNA, Small Interfering, Secretory Pathway, rab GTP-Binding Proteins

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Human urinary exosomes in bladder cancer patients: properties, concentrations and possible clinical application

    Riches, A. C., Powis, S. J., Mullen, P., Harrison, D. J., Hacker, C., Lucocq, J. M., Bowness, J. S., Chapman, A., Cameron, R., McLornan, L., Chinn, D. J. & Leung, S., 11 Dec 2015, In : Bladder. 2, 3, 7 p., e19.

    Research output: Contribution to journalArticle

  2. Regulation of exosome release from mammary epithelial and breast cancer cells - a new regulatory pathway

    Riches, A., Campbell, E., Borger, E. & Powis, S., Mar 2014, In : European Journal of Cancer. 50, 5, p. 1025-34 10 p.

    Research output: Contribution to journalArticle

  3. Nanoparticle tracking analysis monitors microvesicle and exosome secretion from immune cells

    Soo, C. Y., Song, Y., Zheng, Y., Campbell, E. C., Riches, A. C., Gunn-Moore, F. & Powis, S. J., Jun 2012, In : Immunology. 136, 2, p. 192-197 6 p.

    Research output: Contribution to journalArticle

  4. Exosomes derived from normal and malignant bladder and prostate cell lines

    Weaver, J., Goodman, C., Kata, S., Nabi, G., Powis, S. & Riches, A., Jun 2011, In : British Journal of Surgery. 98, 6, p. E2-E3 2 p.

    Research output: Contribution to journalAbstract

  5. Nanoparticle tracking analysis of cell exosome and nanovesicle secretion.

    Powis, S. J., Soo, C. Y., Zheng, Y., Campbell, E. C. & Riches, A. C., 2011, In : Microscopy and Analysis. 25, 6, p. 7-9 3 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Mutationally activated K-ras 4A and 4B both mediate lung carcinogenesis

    Patek, C. E., Arends, M. J., Wallace, W. A. H., Luo, F., Hagan, S., Brownstein, D. G., Rose, L., Devenney, P. S., Walker, M., Plowman, S., Berry, R. L., Kolch, W., Sansom, O. J., Harrison, D. J. & Hooper, M. L., 10 Mar 2008, In : Experimental Cell Research. 314, 5, p. 1105-1114 10 p.

    Research output: Contribution to journalArticle

  2. The K-Ras 4A isoform promotes apoptosis but does not affect either lifespan or spontaneous tumor incidence in aging mice

    Plowman, S. J., Arends, M. J., Brownstein, D. G., Luo, F., Devenney, P. S., Rose, L., Ritchie, A-M., Berry, R. L., Harrison, D. J., Hooper, M. L. & Patek, C. E., 1 Jan 2006, In : Experimental Cell Research. 312, 1, p. 16-26 11 p.

    Research output: Contribution to journalArticle

  3. MZF-1 and DbpA interact with DNase I hypersensitive sites that correlate with expression of the human MUC1 mucin gene

    Shiraga, T., Winpenny, J. P., Carter, E. J., McCarthy, V. A., Hollingsworth, M. A. & Harris, A., 1 Aug 2005, In : Experimental Cell Research. 308, 1, p. 41-52 12 p.

    Research output: Contribution to journalArticle

  4. The manganese cation disrupts membrane dynamics along the secretory pathway

    Towler, M. C., Prescott, A. R., James, J., Lucocq, J. M. & Ponnambalam, S., 25 Aug 2000, In : Experimental Cell Research. 259, 1, p. 167-79 13 p.

    Research output: Contribution to journalArticle

ID: 158198753