Skip to content

Research at St Andrews

Multimode fibre: light-sheet microscopy at the tip of a needle

Research output: Contribution to journalArticle

DOI

Open Access permissions

Open

Author(s)

Martin Plöschner, Věra Kollárová, Zbyněk Dostál, Jonathan Nylk, Thomas Barton-Owen, David E. K. Ferrier, Radim Chmelík, Kishan Dholakia, Tomáš Čižmár

School/Research organisations

Abstract

Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre - an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.

Close

Details

Original languageEnglish
Article number18050
Number of pages7
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 14 Dec 2015

    Research areas

  • Plane illumination microscopy, Optical fibers, Deep, Transmission, Beams

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Light-sheet microscopy with attenuation-compensated propagation-invariant beams

    Nylk, J., McCluskey, K. A., Preciado, M., Mazilu, M., Yang, Z., Gunn-Moore, F. J., Aggarwal, S., Tello, J. A., Ferrier, D. E. K. & Dholakia, K., 6 Apr 2018, In : Science Advances. 4, 4, 14 p., eaar4817.

    Research output: Contribution to journalArticle

  2. A compact Airy beam light sheet microscope with a tilted cylindrical lens

    Yang, Z., Prokopas, M., Nylk, J., Coll Llado, C., Gunn-Moore, F. J., Ferrier, D. E. K., Vettenburg, T. & Dholakia, K., 1 Oct 2014, In : Biomedical Optics Express. 5, 10, p. 3434-3442

    Research output: Contribution to journalArticle

  3. Light-sheet microscopy using an Airy beam

    Vettenburg, T., Dalgarno, H. I. C., Nylk, J., Coll-Lladó, C., Ferrier, D. E. K., Cizmar, T., Gunn-Moore, F. J. & Dholakia, K., May 2014, In : Nature Methods. 11, 5, p. 541-544 7 p.

    Research output: Contribution to journalArticle

  4. Light sheet microscopy with acoustic sample confinement

    Yang, Z., Cole, K. L. H., Qiu, Y., Somorjai, I. M. L., Wijesinghe, P., Nylk, J., Cochran, S., Spalding, G. C., Lyons, D. A. & Dholakia, K., 8 Feb 2019, In : Nature Communications. 10, 8 p., 669.

    Research output: Contribution to journalArticle

  5. Three-photon light-sheet fluorescence microscopy

    Escobet Montalban, A., Gasparoli, F. M., Nylk, J., Liu, P., Yang, Z. & Dholakia, K., 1 Nov 2018, In : Optics Letters. 43, 21, p. 5484-5487 4 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Scientific Reports (Journal)

    Monica Arso Civil (Reviewer)
    Jul 2018 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. Scientific Reports (Journal)

    Anouk Margaretha Borst (Reviewer)
    10 Feb 2017

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  3. Scientific Reports (Journal)

    Emma Louise Carroll (Member of editorial board)
    3 Oct 2016

    Activity: Publication peer-review and editorial work typesEditor of research journal

  4. Scientific Reports (Journal)

    Uli Schwarz-Linek (Reviewer)
    11 May 2016

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  5. Scientific Reports (Journal)

    Ildiko Maureen Lara Somorjai (Reviewer)
    2016 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. A comparative analysis of response times shows that multisensory benefits and interactions are not equivalent

    Innes, B. R. & Otto, T. U., 27 Feb 2019, In : Scientific Reports. 9, 10 p., 2921.

    Research output: Contribution to journalArticle

  2. A large explosive silicic eruption in the British Palaeogene Igneous Province

    Troll, V. R., Emeleus, C. H., Nicoll, G. R., Mattsson, T., Ellam, R. M., Donaldson, C. H. & Harris, C., 24 Jan 2019, In : Scientific Reports. 9, 1, 15 p., 494.

    Research output: Contribution to journalArticle

  3. Automated tumour budding quantification by machine learning augments TNM staging in muscle-invasive bladder cancer prognosis

    Brieu, N., Gavriel, C., Nearchou, I. P., Harrison, D. J., Schmidt, G. & Caie, P. D., 26 Mar 2019, In : Scientific Reports. 9, 5174.

    Research output: Contribution to journalArticle

  4. Classifying grey seal behaviour in relation to environmental variability and commercial fishing activity - a multivariate hidden Markov model

    van Beest, F. M., Mews, S., Elkenkamp, S., Schuhmann, P., Tsolak, D., Wobbe, T., Bartolino, V., Bastardie, F., Dietz, R., von Dorrien, C., Galatius, A., Karlsson, O., McConnell, B., Nabe-Nielsen, J., Olsen, M. T., Teilmann, J. & Langrock, R., 4 Apr 2019, In : Scientific Reports. 9, 14 p., 5642.

    Research output: Contribution to journalArticle

  5. Defining the genes for the final steps in biosynthesis of the complex polyketide antibiotic mupirocin by Pseudomonas fluorescens NCIMB10586

    Connolly, J. A., Wilson, A., Macioszek, M., Song, Z., Wang, L., Mohammad, H. H., Yadav, M., di Martino, M., Miller, C. E., Hothersall, J., Haines, A. S., Stephens, E. R., Crump, M. P., Willis, C. L., Simpson, T. J., Winn, P. J. & Thomas, C. M., 7 Feb 2019, In : Scientific Reports. 9, 9 p., 1542.

    Research output: Contribution to journalArticle

ID: 240963406