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Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12

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Janine T Bossé, Yanwen Li, Oystein Angen, Lucy A Weinert, Roy R Chaudhuri, Matt T Holden, Susanna M Williamson, Duncan J Maskell, Alexander W Tucker, Brendan W Wren, Andrew N Rycroft, Paul R Langford, BRaDP1T Consortium, Matthew Holden

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Abstract

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.
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Original languageEnglish
Pages (from-to)2380-2385
Number of pages6
JournalJournal of Clinical Microbiology
Early online date23 Apr 2014
DOIs
Publication statusPublished - Jul 2014

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