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Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12

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Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12. / Bossé, Janine T; Li, Yanwen; Angen, Oystein; Weinert, Lucy A; Chaudhuri, Roy R; Holden, Matt T; Williamson, Susanna M; Maskell, Duncan J; Tucker, Alexander W; Wren, Brendan W; Rycroft, Andrew N; Langford, Paul R; BRaDP1T Consortium ; Holden, Matthew.

In: Journal of Clinical Microbiology, 07.2014, p. 2380-2385.

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Harvard

Bossé, JT, Li, Y, Angen, O, Weinert, LA, Chaudhuri, RR, Holden, MT, Williamson, SM, Maskell, DJ, Tucker, AW, Wren, BW, Rycroft, AN, Langford, PR, BRaDP1T Consortium & Holden, M 2014, 'Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12', Journal of Clinical Microbiology, pp. 2380-2385. https://doi.org/10.1128/JCM.00685-14

APA

Bossé, J. T., Li, Y., Angen, O., Weinert, L. A., Chaudhuri, R. R., Holden, M. T., ... Holden, M. (2014). Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12. Journal of Clinical Microbiology, 2380-2385. https://doi.org/10.1128/JCM.00685-14

Vancouver

Bossé JT, Li Y, Angen O, Weinert LA, Chaudhuri RR, Holden MT et al. Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12. Journal of Clinical Microbiology. 2014 Jul;2380-2385. https://doi.org/10.1128/JCM.00685-14

Author

Bossé, Janine T ; Li, Yanwen ; Angen, Oystein ; Weinert, Lucy A ; Chaudhuri, Roy R ; Holden, Matt T ; Williamson, Susanna M ; Maskell, Duncan J ; Tucker, Alexander W ; Wren, Brendan W ; Rycroft, Andrew N ; Langford, Paul R ; BRaDP1T Consortium ; Holden, Matthew. / Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12. In: Journal of Clinical Microbiology. 2014 ; pp. 2380-2385.

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@article{aa9f986379c44589985447de330cd3c4,
title = "Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12",
abstract = "An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.",
author = "Boss{\'e}, {Janine T} and Yanwen Li and Oystein Angen and Weinert, {Lucy A} and Chaudhuri, {Roy R} and Holden, {Matt T} and Williamson, {Susanna M} and Maskell, {Duncan J} and Tucker, {Alexander W} and Wren, {Brendan W} and Rycroft, {Andrew N} and Langford, {Paul R} and {BRaDP1T Consortium} and Matthew Holden",
note = "This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G003203/1) and the United Kingdom Department for Environment, Food and Rural Af-fairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.",
year = "2014",
month = "7",
doi = "10.1128/JCM.00685-14",
language = "English",
pages = "2380--2385",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12

AU - Bossé, Janine T

AU - Li, Yanwen

AU - Angen, Oystein

AU - Weinert, Lucy A

AU - Chaudhuri, Roy R

AU - Holden, Matt T

AU - Williamson, Susanna M

AU - Maskell, Duncan J

AU - Tucker, Alexander W

AU - Wren, Brendan W

AU - Rycroft, Andrew N

AU - Langford, Paul R

AU - BRaDP1T Consortium

AU - Holden, Matthew

N1 - This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G003203/1) and the United Kingdom Department for Environment, Food and Rural Af-fairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.

PY - 2014/7

Y1 - 2014/7

N2 - An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

AB - An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

U2 - 10.1128/JCM.00685-14

DO - 10.1128/JCM.00685-14

M3 - Article

SP - 2380

EP - 2385

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

ER -

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