Skip to content

Research at St Andrews

Muscarinic modulation of the Xenopus laevis tadpole spinal mechanosensory pathway

Research output: Contribution to journalArticlepeer-review

Abstract

Muscarinic acetylcholine receptors (mAChRs) mediate effects of acetylcholine (ACh) in many systems, including those involved in spinal functions like locomotion. In Xenopus laevis tadpoles at two days old, a model vertebrate for motor control research, we investigated the role of mAChRs in the skin mechanosensory pathway. We found that mAChR activation by carbachol did not affect the sensory Rohon-Beard neuron properties. However, carbachol could hyperpolarise sensory interneurons and decrease their voltage responses to outward currents. Carbachol could increase the threshold for the mechanosensory pathway to start swimming, preventing the initiation of swimming at higher concentrations altogether. Recording from the sensory interneurons in carbachol showed that their spiking after skin stimulation was depressed. However, the general muscarinic antagonist atropine did not have a clear effect on the swimming threshold or the modulation of sensory interneuron membrane conductance. Our results suggest the skin mechanosensory pathway may be subject to muscarinic modulation in this simple vertebrate system.
Close

Details

Original languageEnglish
Pages (from-to)278-284
JournalBrain Research Bulletin
Volume139
Early online date27 Mar 2018
DOIs
Publication statusPublished - May 2018

    Research areas

  • Xenopus laevis, Swimming, Muscarinic receptor, Rectification, Carbachol, Mechanosensory

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Making in situ whole-cell patch-clamp recordings from Xenopus laevis tadpole neurons

    Li, W-C., 3 Feb 2021, In: Cold Spring Harbor Protocols.

    Research output: Contribution to journalArticlepeer-review

  2. The neuronal mechanisms underlying locomotion termination

    Ritson, E. J. & Li, W-C., Apr 2019, In: Current Opinion in Physiology. 8, p. 109-115 7 p.

    Research output: Contribution to journalReview articlepeer-review

  3. The decision to move: response times, neuronal circuits and sensory memory in a simple vertebrate

    Roberts, A., Borisyuk, R., Buhl, E., Ferrario, A., Koutsikou, S., Li, W-C. & Soffe, S. R., Mar 2019, In: Proceedings of the Royal Society B: Biological Sciences. 286, 1899, 7 p., 20190297.

    Research output: Contribution to journalReview articlepeer-review

  4. Stimulation of single, possible CHX10 hindbrain neurons turns swimming on and off in young Xenopus tadpoles

    Li, W-C. & Soffe, S., 18 Feb 2019, In: Frontiers in Cellular Neuroscience. 13, 12 p., 47.

    Research output: Contribution to journalArticlepeer-review

  5. A simple decision to move in response to touch reveals basic sensory memory and mechanisms for variable response times

    Koutsikou, S., Merrison-Hort, R., Buhl, E., Ferrario, A., Li, W-C., Borisyuk, R., Soffe, S. R. & Roberts, A., 15 Dec 2018, In: The Journal of Physiology. 596, 24, p. 6219-6233

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. The effects of excitotoxic lesions of the pedunculopontine tegmental nucleus on conditioned place preference to 4%, 12% and 20% sucrose solutions

    Alderson, H. L., Jenkins, TA., Kozak, R., Latimer, MP. & Winn, P., Dec 2001, In: Brain Research Bulletin. 56, 6, p. 599-605 7 p.

    Research output: Contribution to journalArticlepeer-review

  2. Electrical interaction between neurons in the pigeon isthmo-optic nucleus.

    Li, W., Li, W-C., Xiao, Q. & Wang, S-R., 2000, In: Brain Research Bulletin. 51, p. 159-163

    Research output: Contribution to journalArticlepeer-review

  3. The development of neuromodulatory systems and the maturation of motor patterns in amphibian tadpoles

    McLean, DL., Merrywest, SD. & Sillar, K. T., 15 Nov 2000, In: Brain Research Bulletin. 53, 5, p. 595-603 9 p.

    Research output: Contribution to journalArticlepeer-review

  4. Tectal afferents monosynaptically activate neurons in the pigeon isthmo-optic nucleus.

    Li, W., Hu, J. & Wang, S-R., Jun 1999, In: Brain Research Bulletin. 49, p. 203-208 6 p.

    Research output: Contribution to journalArticlepeer-review

ID: 252648966

Top