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Mutations in the mitochondrial orf108 render Moricandia arvensis restorer ineffective in restoring male fertility to Brassica oxyrrhina-based cytoplasmic male sterile line of B. juncea

Research output: Contribution to journalArticle


Vasupalli Naresh, Sunil Kumar Singh, Anshul Watts, Pankaj Kumar, Vajinder Kumar, K. R. S. Sambasiva Rao, Shripad Ramachandra Bhat

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We reported earlier an evolutionarily conserved mitochondrial orf108 causing cytoplasmic male sterility (CMS) in four alloplasmic lines of Brassica juncea. B. oxyrrhina also carries orf108, but male sterility of B. oxyrrhina-based B. juncea CMS lines is not restored by the Moricandia arvensis restorer that rescues other orf108-containing B. juncea CMS lines. To understand this discrepancy, we characterized B. oxyrrhina-based B. juncea CMS line (oxy-cms) to identify the mitochondrial gene associated with male sterility. Examination of expression patterns of 26 mitochondrial genes in CMS and male fertile (oxy-camp, i.e. amphidiploid B. oxyrrhina × B. rapa, and euplasmic B. juncea) lines revealed polymorphic transcript patterns for six genes: atp1, atp4, ccmfn2, cox3, nad4L and orf108. Detailed analysis showed that orf108 is linked and co-transcribed with atp1. Comparison of orf108-atp1 transcripts in male sterile and male fertile flowers revealed that B. oxyrrhina restorer cleaves orf108-atp1 at a different location within the orf108 coding region as compared with the M. arvensis restorer. A total of eleven SNPs were detected within the orf108 coding region. One of these SNPs was located at the site where M. arvensis restorer processes the orf108-atp1 transcript suggesting that it could be critical for cleavage of orf108-atp1 transcript by the M. arvensis restorer.



Original languageEnglish
Article number67
Number of pages9
JournalMolecular Breeding
Issue number6
Early online date23 May 2016
Publication statusPublished - 1 Jun 2016

    Research areas

  • 5′ RACE, CMS, Fertility restoration, Single nucleotide polymorphism, Transcript processing

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