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Mycobacterial load assay

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Author(s)

Stephen H Gillespie, Wilber Sabiiti, Katarina Oravcova

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Abstract

Tuberculosis is a difficult disease to treat, a process made more harder as tools to monitor treatment response only provide a result long after the patient has provided a sample. The mycobacterial load assay (MBLA) provides a simple molecular test to quantify and determine the viability of M. tuberculosis in human or other samples.

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Details

Original languageEnglish
Title of host publicationDiagnostic Bacteriology
Subtitle of host publicationMethods and Protocols
EditorsKimberley A. Bishop-Lilly
Place of PublicationNew York, NY
PublisherHumana Press/Springer
Pages89-105
Number of pages17
ISBN (Electronic)9781493970377
ISBN (Print)9781493970353
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1616
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

    Research areas

  • Mycobacterial load assay (MBLA), Sputum, Ribosomal RNA (rRNA), Reverse transcriptase real-time quantitative polymerase chain reaction (RT-qPCR)

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    Sabiiti, W., Mtafya, B. A., Alferes De Lima, D., Dombay, E., Baron, V. O., Azam, K., Orascova, K., Sloan, D. J. & Gillespie, S. H., 6 Sep 2019, (Accepted/In press) In : Journal of Visualized Experiments.

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  2. Model-based relationship between the molecular bacterial load assay and time-to-positivity in liquid culture

    Svensson, R. J., Sabiiti, W., Kibiki, G. S., Ntinginya, N. E., Bhatt, N., Davies, G., Gillespie, S. H. & Simonsson, U. S. H., 29 Jul 2019, In : Antimicrobial Agents and Chemotherapy. Early

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  3. Molecular bacterial load assay (MBLA) concurs with culture on the NaOH-induced Mycobacterium tuberculosis loss of viability

    Mtafya, B., Sabiiti, W., Sabi, I., John, J., Sichone, E., Ntinginya, N. E. & Gillespie, S. H., 25 Jun 2019, In : Journal of Clinical Microbiology. 57, e01992-18.

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  4. Heat-inactivation renders sputum safe and preserves Mycobacterium tuberculosis RNA for downstream molecular tests

    Sabiiti, W., Azam, K., Esmeraldo, E., Bhatt, N., Rachow, A. & Gillespie, S. H., Mar 2019, In : Journal of Clinical Microbiology. 57, 4, 8 p., e01778-18.

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  5. OMNIgene.SPUTUM suppresses contaminants whilst maintaining Mycobacterium tuberculosis viability and obviates cold-chain transport

    Azam, K., Cadir, N., Madeira, C., Gillespie, S. H. & Sabiiti, W., 16 Feb 2018, In : ERJ Open Research. 4, 8 p., 00074-2017.

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ID: 250685143

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