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Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy

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Nanoparticle suspensions enclosed in methylcellulose : a new approach for quantifying nanoparticles in transmission electron microscopy. / Hacker, Christian; Asadi, Jalal; Pliotas, Christos; Ferguson, Sophie Grace Alicia; Sherry, Lee; Marius, Phedra; Tello, Javier; Jackson, David; Naismith, James Henderson; Lucocq, John Milton.

In: Scientific Reports, Vol. 6, 25275 , 04.05.2016.

Research output: Contribution to journalArticlepeer-review

Harvard

Hacker, C, Asadi, J, Pliotas, C, Ferguson, SGA, Sherry, L, Marius, P, Tello, J, Jackson, D, Naismith, JH & Lucocq, JM 2016, 'Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy', Scientific Reports, vol. 6, 25275 . https://doi.org/10.1038/srep25275

APA

Hacker, C., Asadi, J., Pliotas, C., Ferguson, S. G. A., Sherry, L., Marius, P., Tello, J., Jackson, D., Naismith, J. H., & Lucocq, J. M. (2016). Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy. Scientific Reports, 6, [25275 ]. https://doi.org/10.1038/srep25275

Vancouver

Hacker C, Asadi J, Pliotas C, Ferguson SGA, Sherry L, Marius P et al. Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy. Scientific Reports. 2016 May 4;6. 25275 . https://doi.org/10.1038/srep25275

Author

Hacker, Christian ; Asadi, Jalal ; Pliotas, Christos ; Ferguson, Sophie Grace Alicia ; Sherry, Lee ; Marius, Phedra ; Tello, Javier ; Jackson, David ; Naismith, James Henderson ; Lucocq, John Milton. / Nanoparticle suspensions enclosed in methylcellulose : a new approach for quantifying nanoparticles in transmission electron microscopy. In: Scientific Reports. 2016 ; Vol. 6.

Bibtex - Download

@article{fddadfcfc62b4c6595058c50a1f44c77,
title = "Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy",
abstract = "Nanoparticles are of increasing importance in biomedicine but quantification is problematic because current methods depend on indirect measurements at low resolution. Here we describe a new high-resolution method for measuring and quantifying nanoparticles in suspension. It involves premixing nanoparticles in a hydrophilic support medium (methylcellulose) before introducing heavy metal stains for visualization in small air-dried droplets by transmission electron microscopy (TEM). The use of methylcellulose avoids artifacts of conventional negative stain-TEM by (1) restricting interactions between the nanoparticles, (2) inhibiting binding to the specimen support films and (3) reducing compression after drying. Methylcellulose embedment provides effective electron imaging of liposomes, nanodiscs and viruses as well as comprehensive visualization of nanoparticle populations in droplets of known size. These qualities facilitate unbiased sampling, rapid size measurement and estimation of nanoparticle numbers by means of ratio counting using a colloidal gold calibrant. Specimen preparation and quantification take minutes and require a few microliters of sample using only basic laboratory equipment and a standard TEM.",
keywords = "Electron microscopy, Liposome, Methylcellulose, Nanoparticles, Negative stain, Quantification, Stereology, Virus",
author = "Christian Hacker and Jalal Asadi and Christos Pliotas and Ferguson, {Sophie Grace Alicia} and Lee Sherry and Phedra Marius and Javier Tello and David Jackson and Naismith, {James Henderson} and Lucocq, {John Milton}",
note = "This work was supported by the University of St Andrews and Nanomorphomics group funds. The work forms part of an International Patent Application No. PCT/GB2015/052482.",
year = "2016",
month = may,
day = "4",
doi = "10.1038/srep25275",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature publishing group",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Nanoparticle suspensions enclosed in methylcellulose

T2 - a new approach for quantifying nanoparticles in transmission electron microscopy

AU - Hacker, Christian

AU - Asadi, Jalal

AU - Pliotas, Christos

AU - Ferguson, Sophie Grace Alicia

AU - Sherry, Lee

AU - Marius, Phedra

AU - Tello, Javier

AU - Jackson, David

AU - Naismith, James Henderson

AU - Lucocq, John Milton

N1 - This work was supported by the University of St Andrews and Nanomorphomics group funds. The work forms part of an International Patent Application No. PCT/GB2015/052482.

PY - 2016/5/4

Y1 - 2016/5/4

N2 - Nanoparticles are of increasing importance in biomedicine but quantification is problematic because current methods depend on indirect measurements at low resolution. Here we describe a new high-resolution method for measuring and quantifying nanoparticles in suspension. It involves premixing nanoparticles in a hydrophilic support medium (methylcellulose) before introducing heavy metal stains for visualization in small air-dried droplets by transmission electron microscopy (TEM). The use of methylcellulose avoids artifacts of conventional negative stain-TEM by (1) restricting interactions between the nanoparticles, (2) inhibiting binding to the specimen support films and (3) reducing compression after drying. Methylcellulose embedment provides effective electron imaging of liposomes, nanodiscs and viruses as well as comprehensive visualization of nanoparticle populations in droplets of known size. These qualities facilitate unbiased sampling, rapid size measurement and estimation of nanoparticle numbers by means of ratio counting using a colloidal gold calibrant. Specimen preparation and quantification take minutes and require a few microliters of sample using only basic laboratory equipment and a standard TEM.

AB - Nanoparticles are of increasing importance in biomedicine but quantification is problematic because current methods depend on indirect measurements at low resolution. Here we describe a new high-resolution method for measuring and quantifying nanoparticles in suspension. It involves premixing nanoparticles in a hydrophilic support medium (methylcellulose) before introducing heavy metal stains for visualization in small air-dried droplets by transmission electron microscopy (TEM). The use of methylcellulose avoids artifacts of conventional negative stain-TEM by (1) restricting interactions between the nanoparticles, (2) inhibiting binding to the specimen support films and (3) reducing compression after drying. Methylcellulose embedment provides effective electron imaging of liposomes, nanodiscs and viruses as well as comprehensive visualization of nanoparticle populations in droplets of known size. These qualities facilitate unbiased sampling, rapid size measurement and estimation of nanoparticle numbers by means of ratio counting using a colloidal gold calibrant. Specimen preparation and quantification take minutes and require a few microliters of sample using only basic laboratory equipment and a standard TEM.

KW - Electron microscopy

KW - Liposome

KW - Methylcellulose

KW - Nanoparticles

KW - Negative stain

KW - Quantification

KW - Stereology

KW - Virus

U2 - 10.1038/srep25275

DO - 10.1038/srep25275

M3 - Article

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 25275

ER -

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