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Research at St Andrews

Narrowband organic light-emitting diodes for fluorescence microscopy and calcium imaging

Research output: Contribution to journalArticle

DOI

Open Access Status

  • Embargoed (until 5/09/20)

Author(s)

Caroline Murawski, Andreas Mischok, Jonathan Hunter Booth, Jothi Dinesh Kumar, Emily Archer, Laura Christine Tropf, Changmin Keum, Yali Deng, Kou Yoshida, Ifor David William Samuel, Marcel Schubert, Stefan Pulver, Malte Christian Gather

School/Research organisations

Abstract

Fluorescence imaging is an indispensable tool in biology, with applications ranging from single‐cell to whole‐animal studies and with live mapping of neuronal activity currently receiving particular attention. To enable fluorescence imaging at cellular scale in freely moving animals, miniaturized microscopes and lensless imagers are developed that can be implanted in a minimally invasive fashion; but the rigidity, size, and potential toxicity of the involved light sources remain a challenge. Here, narrowband organic light‐emitting diodes (OLEDs) are developed and used for fluorescence imaging of live cells and for mapping of neuronal activity in Drosophila melanogaster via genetically encoded Ca2+ indicators. In order to avoid spectral overlap with fluorescence from the sample, distributed Bragg reflectors are integrated onto the OLEDs to block their long‐wavelength emission tail, which enables an image contrast comparable to conventional, much bulkier mercury light sources. As OLEDs can be fabricated on mechanically flexible substrates and structured into arrays of cell‐sized pixels, this work opens a new pathway for the development of implantable light sources that enable functional imaging and sensing in freely moving animals.
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Details

Original languageEnglish
Number of pages8
JournalAdvanced Materials
VolumeEarly View
Early online date5 Sep 2019
DOIs
Publication statusE-pub ahead of print - 5 Sep 2019

    Research areas

  • Calcium imaging, Distributed Bragg reflector, Fluorescence microscopy, OLED

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