Skip to content

Research at St Andrews

Native electrospray mass spectrometry approaches to probe the interaction between zinc and an anti-angiogenic peptide from histidine-rich glycoprotein

Research output: Contribution to journalArticle

Author(s)

Esther M. Martin, Frances D. L. Kondrat, Alan J. Stewart, James H. Scrivens, Peter J. Sadler, Claudia A. Blindauer

School/Research organisations

Abstract

Zinc modulates the biological function of histidine-rich glycoprotein (HRG) through binding to its His-rich region (HRR). The Zn2+-binding properties of a 35 amino-acid biologically-active peptide mimic of the HRR, HRGP330, were investigated using dissociative mass spectrometry approaches in addition to travelling-wave ion mobility mass spectrometry (TWIM-MS). Native mass spectrometry confirmed zinc binding to HRGP330; however, broadening of the 1H NMR resonances upon addition of Zn2+ ions precluded the attainment of structural information. A complementary approach employing TWIM-MS indicated that HRGP330 has a more compact structure in the presence of Zn2+ ions. Top-down MS/MS data supported a metal-binding-induced conformational change, as fewer fragments were observed for Zn2+-bound HRGP330. Zn2+-bound fragments of both N-terminal and C-terminal ends of the peptide were identified from collision-induced dissociation (CID) and electron transfer dissociation/proton transfer reaction (ETD/PTR) experiments, suggesting that multiple binding sites exist within this region of HRG. The combination of mass spectrometry and NMR approaches provides new insight into the highly dynamic interaction between zinc and this His-rich peptide.
Close

Details

Original languageEnglish
Article number8646
Number of pages13
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 5 Jun 2018

    Research areas

  • Ion mobility mass spectrometry, Top-down mass spectrometry, Zinc, Histidine-rich glycoprotein

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The X files: ''The mystery of X chromosome instability in Alzheimer's disease''

    Bajic, V. P., Essak, M., Zivkovic, L., Stewart, A. J., Zafirovic, S., Bajic, V., Gojobori, T., Isenovic, E. & Spremo-Potparevic, B., 13 Dec 2019, (Accepted/In press) In : Frontiers in Genetics.

    Research output: Contribution to journalReview article

  2. Coagulatory defects in type-1 and type-2 diabetes

    Sobczak, A. I. S. & Stewart, A. J., 12 Dec 2019, (Accepted/In press) In : International Journal of Molecular Sciences.

    Research output: Contribution to journalReview article

  3. Reduced plasma magnesium levels in type-1 diabetes associate with prothrombotic changes in fibrin clotting and fibrinolysis

    Sobczak, A. I. S., Phoenix, F. A., Pitt, S. J., Ajjan, R. A. & Stewart, A. J., 11 Nov 2019, (Accepted/In press) In : Thrombosis and Haemostasis.

    Research output: Contribution to journalArticle

  4. A metalloproteomic analysis of interactions between plasma proteins and zinc: elevated fatty acid levels affect zinc distribution

    Coverdale, J. P. C., Barnett, J. P., Adamu, A. H., Griffiths, E. J., Stewart, A. J. & Blindauer, C. A., 1 Nov 2019, In : Metallomics. 11, 11, p. 1805-1819 15 p.

    Research output: Contribution to journalArticle

  5. Plasma non-esterified fatty acids contribute to increased coagulability in type-2 diabetes through altered plasma zinc speciation

    Sobczak, A. I. S., Katundu, K. G. H., Phoenix, F. A., Khazaipoul, S., Yu, R., Lampiao, F., Stefanowicz, F., Blindauer, C. A., Pitt, S. J., Smith, T. K., Ajjan, R. A. & Stewart, A. J., 28 Aug 2019, In : biorxiv. 38 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Scientific Reports (Journal)

    Alan J. Stewart (Member of editorial board)
    Jun 2019 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  2. Scientific Reports (Journal)

    Monica Arso Civil (Reviewer)
    Jul 2018 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  3. Scientific Reports (Journal)

    Anouk Margaretha Borst (Reviewer)
    10 Feb 2017

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  4. Scientific Reports (Journal)

    Emma Louise Carroll (Member of editorial board)
    3 Oct 2016

    Activity: Publication peer-review and editorial work typesEditor of research journal

  5. Scientific Reports (Journal)

    Uli Schwarz-Linek (Reviewer)
    11 May 2016

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. A comparative analysis of response times shows that multisensory benefits and interactions are not equivalent

    Innes, B. R. & Otto, T. U., 27 Feb 2019, In : Scientific Reports. 9, 10 p., 2921.

    Research output: Contribution to journalArticle

  2. A large explosive silicic eruption in the British Palaeogene Igneous Province

    Troll, V. R., Emeleus, C. H., Nicoll, G. R., Mattsson, T., Ellam, R. M., Donaldson, C. H. & Harris, C., 24 Jan 2019, In : Scientific Reports. 9, 1, 15 p., 494.

    Research output: Contribution to journalArticle

  3. An Escherichia coli ST131 pangenome atlas reveals population structure and evolution across 4,071 isolates

    Decano, A. G. & Downing, T., 22 Nov 2019, In : Scientific Reports. 9, 13 p., 17394.

    Research output: Contribution to journalArticle

  4. Automated tumour budding quantification by machine learning augments TNM staging in muscle-invasive bladder cancer prognosis

    Brieu, N., Gavriel, C., Nearchou, I. P., Harrison, D. J., Schmidt, G. & Caie, P. D., 26 Mar 2019, In : Scientific Reports. 9, 5174.

    Research output: Contribution to journalArticle

  5. Balanced cholinergic modulation of spinal locomotor circuits via M2 and M3 muscarinic receptors

    Nascimento, F., Spindler, L. R. B. & Miles, G. B., 1 Oct 2019, In : Scientific Reports. 9, 16 p., 14051.

    Research output: Contribution to journalArticle

ID: 253108976

Top