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Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility

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Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility. / Krishna, Aishwarya; Holden, Matthew T G; Peacock, Sharon J; Edwards, Andrew M; Wigneshweraraj, Sivaramesh.

In: Microbiology, Vol. 164, No. 9, 2018, p. 1189-1195.

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Krishna, A, Holden, MTG, Peacock, SJ, Edwards, AM & Wigneshweraraj, S 2018, 'Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility', Microbiology, vol. 164, no. 9, pp. 1189-1195. https://doi.org/10.1099/mic.0.000695

APA

Krishna, A., Holden, M. T. G., Peacock, S. J., Edwards, A. M., & Wigneshweraraj, S. (2018). Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility. Microbiology, 164(9), 1189-1195. https://doi.org/10.1099/mic.0.000695

Vancouver

Krishna A, Holden MTG, Peacock SJ, Edwards AM, Wigneshweraraj S. Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility. Microbiology. 2018;164(9):1189-1195. https://doi.org/10.1099/mic.0.000695

Author

Krishna, Aishwarya ; Holden, Matthew T G ; Peacock, Sharon J ; Edwards, Andrew M ; Wigneshweraraj, Sivaramesh. / Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility. In: Microbiology. 2018 ; Vol. 164, No. 9. pp. 1189-1195.

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@article{3b4b19802a0f4e52ac73c52a1dd3acae,
title = "Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility",
abstract = "Nasal colonization by the pathogen Staphylococcus aureus is a risk factor for subsequent infection. Loss of function mutations in the gene encoding the virulence regulator Rsp are associated with the transition of S. aureus from a colonizing isolate to one that causes bacteraemia. Here, we report the identification of several novel activity-altering mutations in rsp detected in clinical isolates, including for the first time, mutations that enhance agr operon activity. We assessed how these mutations affected infection-relevant phenotypes and found loss and enhancement of function mutations to have contrasting effects on S. aureus survival in blood and antibiotic susceptibility. These findings add to the growing body of evidence that suggests S. aureus 'trades off' virulence for the acquisition of traits that benefit survival in the host, and indicates that infection severity and treatment options can be significantly affected by mutations in the virulence regulator rsp.",
keywords = "Virulence regulator, Bacteraemia, Staphylococcus aureas, Rsp, Mutations",
author = "Aishwarya Krishna and Holden, {Matthew T G} and Peacock, {Sharon J} and Edwards, {Andrew M} and Sivaramesh Wigneshweraraj",
note = "This work was funded by the Imperial College President’s Scholarship awarded to A. K; M. T. G.H acknowledges funding from Chief Scientists Office (Grant Number SIRN10); S. J. P thanks the UKCRC Translational Infection Research Initiative and the Medical Research Council (Grant Number G1000803) for funding to cover the original isolate collection; A. M. E acknowledges funding from the Royal Society, Department of Medicine (Imperial College), and from the Imperial NIHR Biomedical Research Centre, Imperial College London; S.W is a recipient of a Wellcome Trust Investigator Award.",
year = "2018",
doi = "10.1099/mic.0.000695",
language = "English",
volume = "164",
pages = "1189--1195",
journal = "Microbiology",
issn = "1350-0872",
publisher = "SOC GENERAL MICROBIOLOGY",
number = "9",

}

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TY - JOUR

T1 - Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility

AU - Krishna, Aishwarya

AU - Holden, Matthew T G

AU - Peacock, Sharon J

AU - Edwards, Andrew M

AU - Wigneshweraraj, Sivaramesh

N1 - This work was funded by the Imperial College President’s Scholarship awarded to A. K; M. T. G.H acknowledges funding from Chief Scientists Office (Grant Number SIRN10); S. J. P thanks the UKCRC Translational Infection Research Initiative and the Medical Research Council (Grant Number G1000803) for funding to cover the original isolate collection; A. M. E acknowledges funding from the Royal Society, Department of Medicine (Imperial College), and from the Imperial NIHR Biomedical Research Centre, Imperial College London; S.W is a recipient of a Wellcome Trust Investigator Award.

PY - 2018

Y1 - 2018

N2 - Nasal colonization by the pathogen Staphylococcus aureus is a risk factor for subsequent infection. Loss of function mutations in the gene encoding the virulence regulator Rsp are associated with the transition of S. aureus from a colonizing isolate to one that causes bacteraemia. Here, we report the identification of several novel activity-altering mutations in rsp detected in clinical isolates, including for the first time, mutations that enhance agr operon activity. We assessed how these mutations affected infection-relevant phenotypes and found loss and enhancement of function mutations to have contrasting effects on S. aureus survival in blood and antibiotic susceptibility. These findings add to the growing body of evidence that suggests S. aureus 'trades off' virulence for the acquisition of traits that benefit survival in the host, and indicates that infection severity and treatment options can be significantly affected by mutations in the virulence regulator rsp.

AB - Nasal colonization by the pathogen Staphylococcus aureus is a risk factor for subsequent infection. Loss of function mutations in the gene encoding the virulence regulator Rsp are associated with the transition of S. aureus from a colonizing isolate to one that causes bacteraemia. Here, we report the identification of several novel activity-altering mutations in rsp detected in clinical isolates, including for the first time, mutations that enhance agr operon activity. We assessed how these mutations affected infection-relevant phenotypes and found loss and enhancement of function mutations to have contrasting effects on S. aureus survival in blood and antibiotic susceptibility. These findings add to the growing body of evidence that suggests S. aureus 'trades off' virulence for the acquisition of traits that benefit survival in the host, and indicates that infection severity and treatment options can be significantly affected by mutations in the virulence regulator rsp.

KW - Virulence regulator

KW - Bacteraemia

KW - Staphylococcus aureas

KW - Rsp

KW - Mutations

U2 - 10.1099/mic.0.000695

DO - 10.1099/mic.0.000695

M3 - Article

VL - 164

SP - 1189

EP - 1195

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 9

ER -

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