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No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio)

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No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio). / Jones, Nick A. R.; Mendo, Tania; Broell, Franziska; Webster, Mike M.

In: Journal of Experimental Biology, Vol. 222, No. 2, jeb.192971, 24.01.2019.

Research output: Contribution to journalArticle

Harvard

Jones, NAR, Mendo, T, Broell, F & Webster, MM 2019, 'No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio)' Journal of Experimental Biology, vol. 222, no. 2, jeb.192971. https://doi.org/10.1242/jeb.192971

APA

Jones, N. A. R., Mendo, T., Broell, F., & Webster, M. M. (2019). No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio). Journal of Experimental Biology, 222(2), [jeb.192971]. https://doi.org/10.1242/jeb.192971

Vancouver

Jones NAR, Mendo T, Broell F, Webster MM. No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio). Journal of Experimental Biology. 2019 Jan 24;222(2). jeb.192971. https://doi.org/10.1242/jeb.192971

Author

Jones, Nick A. R. ; Mendo, Tania ; Broell, Franziska ; Webster, Mike M. / No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio). In: Journal of Experimental Biology. 2019 ; Vol. 222, No. 2.

Bibtex - Download

@article{59dafab0691b4e48be36afd520d2701d,
title = "No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio)",
abstract = "Stress-induced hyperthermia (SIH) is characterised by a rise in body temperature in response to a stressor. In endotherms SIH is mediated by the autonomic nervous system, whereas ectotherms must raise their body temperature via behavioural means by moving to warmer areas within their environment (behavioural thermoregulation). A recent study suggested that zebrafish (Danio rerio), an important model species, may move to warmer water in response to handling and confinement and thus exhibit SIH, which, if accepted, may have important practical and welfare implications. However an alternative hypothesis proposed that the observed movements may been produced by avoidance behaviour rather than behavioural thermoregulation. Investigating the claims for SIH in zebrafish further we conducted two experiments that extend the earlier study. The first experiment incorporated new conditions that considered fish behaviour in the absence of thermal variation, i.e. their null distribution, an important condition that was not performed in the original study. The second was a refined version of the experiment to reduce the numbers of fish and aid movement between areas for the fish. In contrast to the previous study, we saw no effect of handling or confinement on preference for warmer areas, and no evidence for SIH in either experiment. Instead we observed a short-lived reduction in preference for warmer areas immediately post stress. Our work suggests that zebrafish may not experience SIH and claims regarding fish consciousness based on SIH may need to be revised.",
keywords = "Thermal preference, Fish welfare, Emotional fever, Fish stress, Behavioural thermoregulation, Ectotherm thermoregulation",
author = "Jones, {Nick A. R.} and Tania Mendo and Franziska Broell and Webster, {Mike M.}",
note = "NJ was supported by a studentship from The Fisheries Society of The British Isles.",
year = "2019",
month = "1",
day = "24",
doi = "10.1242/jeb.192971",
language = "English",
volume = "222",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio)

AU - Jones, Nick A. R.

AU - Mendo, Tania

AU - Broell, Franziska

AU - Webster, Mike M.

N1 - NJ was supported by a studentship from The Fisheries Society of The British Isles.

PY - 2019/1/24

Y1 - 2019/1/24

N2 - Stress-induced hyperthermia (SIH) is characterised by a rise in body temperature in response to a stressor. In endotherms SIH is mediated by the autonomic nervous system, whereas ectotherms must raise their body temperature via behavioural means by moving to warmer areas within their environment (behavioural thermoregulation). A recent study suggested that zebrafish (Danio rerio), an important model species, may move to warmer water in response to handling and confinement and thus exhibit SIH, which, if accepted, may have important practical and welfare implications. However an alternative hypothesis proposed that the observed movements may been produced by avoidance behaviour rather than behavioural thermoregulation. Investigating the claims for SIH in zebrafish further we conducted two experiments that extend the earlier study. The first experiment incorporated new conditions that considered fish behaviour in the absence of thermal variation, i.e. their null distribution, an important condition that was not performed in the original study. The second was a refined version of the experiment to reduce the numbers of fish and aid movement between areas for the fish. In contrast to the previous study, we saw no effect of handling or confinement on preference for warmer areas, and no evidence for SIH in either experiment. Instead we observed a short-lived reduction in preference for warmer areas immediately post stress. Our work suggests that zebrafish may not experience SIH and claims regarding fish consciousness based on SIH may need to be revised.

AB - Stress-induced hyperthermia (SIH) is characterised by a rise in body temperature in response to a stressor. In endotherms SIH is mediated by the autonomic nervous system, whereas ectotherms must raise their body temperature via behavioural means by moving to warmer areas within their environment (behavioural thermoregulation). A recent study suggested that zebrafish (Danio rerio), an important model species, may move to warmer water in response to handling and confinement and thus exhibit SIH, which, if accepted, may have important practical and welfare implications. However an alternative hypothesis proposed that the observed movements may been produced by avoidance behaviour rather than behavioural thermoregulation. Investigating the claims for SIH in zebrafish further we conducted two experiments that extend the earlier study. The first experiment incorporated new conditions that considered fish behaviour in the absence of thermal variation, i.e. their null distribution, an important condition that was not performed in the original study. The second was a refined version of the experiment to reduce the numbers of fish and aid movement between areas for the fish. In contrast to the previous study, we saw no effect of handling or confinement on preference for warmer areas, and no evidence for SIH in either experiment. Instead we observed a short-lived reduction in preference for warmer areas immediately post stress. Our work suggests that zebrafish may not experience SIH and claims regarding fish consciousness based on SIH may need to be revised.

KW - Thermal preference

KW - Fish welfare

KW - Emotional fever

KW - Fish stress

KW - Behavioural thermoregulation

KW - Ectotherm thermoregulation

U2 - 10.1242/jeb.192971

DO - 10.1242/jeb.192971

M3 - Article

VL - 222

JO - Journal of Experimental Biology

T2 - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

IS - 2

M1 - jeb.192971

ER -

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ID: 257381203