Skip to content

Research at St Andrews

Nonredundant Raman imaging using optical eigenmodes

Research output: Contribution to journalArticle

Author(s)

Sebastian Kosmeier, Svetlana Zolotovskaya, Anna Chiara De Luca, Andrew C Riches, C Simon Herrington, Kishan Dholakia, Michael Mazilu

School/Research organisations

Abstract

Various forms of imaging schemes have emerged over the last decade that are based on correlating variations in incident illuminating light fields to the outputs of single “bucket” detectors. However, to date, the role of the orthogonality of the illumination fields has largely been overlooked, and, furthermore, the field has not progressed beyond bright field imaging. By exploiting the concept of orthogonal illuminating fields, we demonstrate the application of optical eigenmodes (OEis) to wide-field, scan-free spontaneous Raman imaging, which is notoriously slow in wide-field mode. The OEi approach enables a form of indirect imaging that exploits both phase and amplitude in image reconstruction. The use of orthogonality enables us to nonredundantly illuminate the sample and, in particular, use a subset of illuminating modes to obtain the majority of information from the sample, thus minimizing any photobleaching or damage of the sample. The crucial incorporation of phase, in addition to amplitude, in the imaging process significantly reduces background noise and results in an improved signal-to-noise ratio for the image while reducing the number of illuminations. As an example we can reconstruct images of a surface-enhanced Raman spectroscopy sample with approximately an order of magnitude fewer acquisitions. This generic approach may readily be applied to other imaging modalities such as fluorescence microscopy or nonlinear vibrational microscopy.
Close

Details

Original languageEnglish
Pages (from-to)257-263
Number of pages7
JournalOptica
Volume1
Issue number4
Early online date17 Oct 2014
DOIs
Publication statusPublished - 20 Oct 2014

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Optimisation of wavelength modulated Raman spectroscopy: towards high throughput cell screening

    Praveen, B. B., Mazilu, M., Marchington, R. F., Herrington, C. S., Riches, A. & Dholakia, K., 25 Jun 2013, In : PLoS One. 8, 6, 5 p., e67211.

    Research output: Contribution to journalArticle

  2. Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis

    Balagopal, B., Ashok, P. C., Mazilu, M., Riches, A. C., Herrington, C. S. & Dholakia, K., 9 Jul 2012, In : Journal of Biomedical Optics. 17, 7, 6 p., 077006.

    Research output: Contribution to journalArticle

  3. Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples

    Canetta, E., Mazilu, M., De Luca, A. C., Carruthers, A. E., Dholakia, K., Neilson, S., Sargeant, H., Briscoe, T., Herrington, C. S. & Riches, A. C., Mar 2011, In : Journal of Biomedical Optics. 16, 3, 7 p., 037002.

    Research output: Contribution to journalArticle

  4. Optimal algorithm for fluorescence suppression of modulated Raman spectroscopy

    Mazilu, M., De Luca, A. C., Riches, A., Herrington, C. S. & Dholakia, K., 24 May 2010, In : Optics Express. 18, 11, p. 11382-11395 14 p.

    Research output: Contribution to journalArticle

  5. Online Fluorescence Suppression in Modulated Raman Spectroscopy

    De Luca, A. C., Mazilu, M., Riches, A. C., Herrington, C. S. & Dholakia, K., 15 Jan 2010, In : Analytical Chemistry. 82, 2, p. 738-745 8 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Low-threshold polariton lasing in a highly disordered conjugated polymer

    Wei, M., Rajendran, S. K., Ohadi, H., Tropf, L. C., Gather, M. C., Turnbull, G. A. & Samuel, I. D. W., 27 Aug 2019, In : Optica. 6, 9, p. 1124-1129 6 p., 362590.

    Research output: Contribution to journalArticle

  2. Monolithic frequency comb platform based on interband cascade lasers and detectors

    Schwarz, B., Hillbrand, J., Beiser, M., Andrews, A. M., Strasser, G., Detz, H., Schade, A., Weih, R. & Höfling, S., 12 Jul 2019, In : Optica. 6, 7, p. 890-895 6 p.

    Research output: Contribution to journalArticle

  3. Picosecond pulses from a mid-infrared interband cascade laser

    Hillbrand, J., Beiser, M., Andrews, A. M., Detz, H., Weih, R., Schade, A., Höfling, S., Strasser, G. & Schwarz, B., 20 Oct 2019, In : Optica. 6, 10, p. 1334-1337 4 p.

    Research output: Contribution to journalArticle

  4. Quantum frequency conversion of a quantum dot single-photon source on a nanophotonic chip

    Singh, A., Li, Q., Liu, S., Yu, Y., Lu, X., Schneider, C., Höfling, S., Lawall, J., Verma, V., Mirin, R., Nam, S. W., Liu, J. & Srinivasan, K., May 2019, In : Optica. 6, 5, p. 563-569 7 p.

    Research output: Contribution to journalArticle

  5. Rapid broadband characterization of scattering medium using hyperspectral imaging

    Boniface, A., Gusachenko, I., Dholakia, K. & Gigan, S., 20 Mar 2019, In : Optica. 6, 3, p. 274-279

    Research output: Contribution to journalArticle

ID: 163898288

Top