Skip to content

Research at St Andrews

Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells

Research output: Contribution to journalArticlepeer-review

DOI

Open Access permissions

Open

Author(s)

Luis Alberto Baena-Lopez, Lewis Arthurton, Marcus Bischoff, Jean-Paul Vincent, Cyrille Alexandre, Reuben McGregor

School/Research organisations

Abstract

The caspase-mediated regulation of many cellular processes, including apoptosis, justifies the substantial interest in understanding all of the biological features of these enzymes. To complement functional assays, it is crucial to identify caspase-activating cells in live tissues. Our work describes novel initiator caspase reporters that, for the first time, provide direct information concerning the initial steps of the caspase activation cascade in Drosophila tissues. One of our caspase sensors capitalises on the rapid subcellular localisation change of a fluorescent marker to uncover novel cellular apoptotic events relating to the actin-mediated positioning of the nucleus before cell delamination. The other construct benefits from caspase-induced nuclear translocation of a QF transcription factor. This feature enables the genetic manipulation of caspase-activating cells and reveals the spatiotemporal patterns of initiator caspase activity. Collectively, our sensors offer experimental opportunities not available by using previous reporters and have proven useful to illuminate previously unknown aspects of caspase-dependent processes in apoptotic and non-apoptotic cellular scenarios.
Close

Details

Original languageEnglish
Article numberdev170811
Number of pages11
JournalDevelopment
Volume145
Issue number23
Early online date9 Nov 2018
DOIs
Publication statusPublished - 4 Dec 2018

    Research areas

  • Apical caspase reporter, Apoptotic nuclear migration, Historical caspase reporter, Interkinetic nuclear migration, Non-apoptotic caspase functions

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis

    Pulido Companys, P., Norris, A. & Bischoff, M., 30 Mar 2020, In: Journal of Cell Science. 133, 6, 18 p., jcs235325.

    Research output: Contribution to journalArticlepeer-review

  2. In Vivo Imaging of Hedgehog Transport in Drosophila Epithelia

    Seijo, I., Guerrero, I. & Bischoff, M., 2015, Methods in Molecular Biology: Hedgehog Signalling Protocols. Riobo, N. (ed.). Springer Science and Business Media, Vol. 1322.

    Research output: Chapter in Book/Report/Conference proceedingChapter

  3. Exosomes as Hedgehog carriers in cytoneme-mediated transport and secretion

    Gradilla, A-C., González, E., Seijo, I., Andrés, G., Bischoff, M., González-Mendez, L., Sánchez, V., Callejo, A., Ibáñez, C., Guerra, M., Ortigão-Farias, J. R., Sutherland, J. D., González, M., Barrio, R., Falcón-Pérez, J. M. & Guerrero, I., 4 Dec 2014, In: Nature Communications. 5, 13 p., 5649.

    Research output: Contribution to journalArticlepeer-review

  4. Epidermal growth factor signalling controls Myosin II planar polarity to orchestrate convergent extension movements during Drosophila tubulogenesis

    Saxena, A., Denholm, B., Bunt, S., Bischoff, M., VijayRaghavan, K. & Skaer, H., 2 Dec 2014, In: PLoS Biology. 12, 12, 17 p., e1002013.

    Research output: Contribution to journalArticlepeer-review

  5. The conserved transmembrane proteoglycan Perdido/Kon-tiki is essential for myofibrillogenesis and sarcomeric structure in Drosophila

    Pérez-Moreno, J. J., Bischoff, M., Martín-Bermudo, M. D. & Estrada, B., Jul 2014, In: Journal of Cell Science. 127, p. 3162-3173

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. Development (Journal)

    John Barry Tucker (Editor)

    19781988

    Activity: Publication peer-review and editorial work typesEditor of research journal

Related by journal

  1. Evolutionary crossroads in developmental biology: annelids.

    Ferrier, D. E. K., 2012, In: Development. 139, p. 2643-2653

    Research output: Contribution to journalReview articlepeer-review

  2. Cell rearrangements, cell divisions and cell death in a migrating epithelial sheet in the abdomen of Drosophila

    Bischoff, M. & Cseresnyes, Z., 15 Jul 2009, In: Development. 136, 14, p. 2403-2411 9 p.

    Research output: Contribution to journalArticlepeer-review

  3. Formation of the embryonic-abembryonic axis of the mouse blastocyst: relationships between orientation of early cleavage divisions and pattern of symmetric/asymmetric divisions

    Bischoff, M., Parfitt, D-E. & Zernicka-Goetz, M., Mar 2008, In: Development. 135, 5, p. 953-962 10 p.

    Research output: Contribution to journalArticlepeer-review

  4. Populations of NGF-dependent neurons differ in their requirement for BAX to undergo apoptosis in the absence of NGF/TrkA signalling in vivo

    Middleton, G. H. & Davies, AM., Dec 2001, In: Development. 128, p. 4715-4728 14 p.

    Research output: Contribution to journalArticlepeer-review

ID: 257289666

Top