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Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells

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Author(s)

Luis Alberto Baena-Lopez, Lewis Arthurton, Marcus Bischoff, Jean-Paul Vincent, Cyrille Alexandre, Reuben McGregor

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Abstract

The caspase-mediated regulation of many cellular processes, including apoptosis, justifies the substantial interest in understanding all of the biological features of these enzymes. To complement functional assays, it is crucial to identify caspase-activating cells in live tissues. Our work describes novel initiator caspase reporters that, for the first time, provide direct information concerning the initial steps of the caspase activation cascade in Drosophila tissues. One of our caspase sensors capitalises on the rapid subcellular localisation change of a fluorescent marker to uncover novel cellular apoptotic events relating to the actin-mediated positioning of the nucleus before cell delamination. The other construct benefits from caspase-induced nuclear translocation of a QF transcription factor. This feature enables the genetic manipulation of caspase-activating cells and reveals the spatiotemporal patterns of initiator caspase activity. Collectively, our sensors offer experimental opportunities not available by using previous reporters and have proven useful to illuminate previously unknown aspects of caspase-dependent processes in apoptotic and non-apoptotic cellular scenarios.
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Original languageEnglish
Number of pages11
JournalDevelopment
Volume145
Issue number23
Early online date9 Nov 2018
DOIs
Publication statusPublished - 4 Dec 2018

    Research areas

  • Apical caspase reporter, Apoptotic nuclear migration, Historical caspase reporter, Interkinetic nuclear migration, Non-apoptotic caspase functions

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