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Obtaining accurate glucose measurements from wild animals under field conditions: comparing a hand held glucometer with a standard laboratory technique in grey seals

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Obtaining accurate glucose measurements from wild animals under field conditions : comparing a hand held glucometer with a standard laboratory technique in grey seals. / Bennett, Kimberley A.; Turner, Lucy M.; Millward, Sebastian; Moss, Simon E. W.; Hall, Ailsa J.

In: Conservation Physiology, Vol. 5, No. 1, 27.02.2017.

Research output: Contribution to journalArticle

Harvard

Bennett, KA, Turner, LM, Millward, S, Moss, SEW & Hall, AJ 2017, 'Obtaining accurate glucose measurements from wild animals under field conditions: comparing a hand held glucometer with a standard laboratory technique in grey seals' Conservation Physiology, vol. 5, no. 1. https://doi.org/10.1093/conphys/cox013

APA

Bennett, K. A., Turner, L. M., Millward, S., Moss, S. E. W., & Hall, A. J. (2017). Obtaining accurate glucose measurements from wild animals under field conditions: comparing a hand held glucometer with a standard laboratory technique in grey seals. Conservation Physiology, 5(1). https://doi.org/10.1093/conphys/cox013

Vancouver

Bennett KA, Turner LM, Millward S, Moss SEW, Hall AJ. Obtaining accurate glucose measurements from wild animals under field conditions: comparing a hand held glucometer with a standard laboratory technique in grey seals. Conservation Physiology. 2017 Feb 27;5(1). https://doi.org/10.1093/conphys/cox013

Author

Bennett, Kimberley A. ; Turner, Lucy M. ; Millward, Sebastian ; Moss, Simon E. W. ; Hall, Ailsa J. / Obtaining accurate glucose measurements from wild animals under field conditions : comparing a hand held glucometer with a standard laboratory technique in grey seals. In: Conservation Physiology. 2017 ; Vol. 5, No. 1.

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@article{1bb3cc350738411e949a0c574be1c4df,
title = "Obtaining accurate glucose measurements from wild animals under field conditions: comparing a hand held glucometer with a standard laboratory technique in grey seals",
abstract = "Glucose is an important metabolic fuel and circulating levels are tightly regulated in most mammals, but can drop when body fuel reserves become critically low. Glucose is mobilized rapidly from liver and muscle during stress in response to increased circulating cortisol. Blood glucose levels can thus be of value in conservation as an indicator of nutritional status and may be a useful, rapid assessment marker for acute or chronic stress. However, seals show unusual glucose regulation: circulating levels are high and insulin sensitivity is limited. Accurate blood glucose measurement is therefore vital to enable meaningful health and physiological assessments in captive, wild or rehabilitated seals and to explore its utility as a marker of conservation relevance in these animals. Point-of-care devices are simple, portable, relatively cheap and use less blood compared with traditional sampling approaches, making them useful in conservation-related monitoring. We investigated the accuracy of a hand-held glucometer for ‘instant’ field measurement of blood glucose, compared with blood drawing followed by laboratory testing, in wild grey seals (Halichoerus grypus), a species used as an indicator for Good Environmental Status in European waters. The glucometer showed high precision, but low accuracy, relative to laboratory measurements, and was least accurate at extreme values. It did not provide a reliable alternative to plasma analysis. Poor correlation between methods may be due to suboptimal field conditions, greater and more variable haematocrit, faster erythrocyte settling rate and/or lipaemia in seals. Glucometers must therefore be rigorously tested before use in new species and demographic groups. Sampling, processing and glucose determination methods have major implications for conclusions regarding glucose regulation, and health assessment in seals generally, which is important in species of conservation concern and in development of circulating glucose as a marker of stress or nutritional state for use in management and monitoring.",
keywords = "Glucose, Glucometer, Phocid, Pinniped, Point-of-care, Validation",
author = "Bennett, {Kimberley A.} and Turner, {Lucy M.} and Sebastian Millward and Moss, {Simon E. W.} and Hall, {Ailsa J.}",
note = "This work was supported in part by National Capability funding to Sea Mammal Research Unit, University of St Andrews (Grant No. SMRU 1001) from the UK Natural Environment Research Council. L.M.T. was supported by internal funding from Plymouth University.",
year = "2017",
month = "2",
day = "27",
doi = "10.1093/conphys/cox013",
language = "English",
volume = "5",
journal = "Conservation Physiology",
issn = "2051-1434",
publisher = "Oxford University Press",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Obtaining accurate glucose measurements from wild animals under field conditions

T2 - Conservation Physiology

AU - Bennett, Kimberley A.

AU - Turner, Lucy M.

AU - Millward, Sebastian

AU - Moss, Simon E. W.

AU - Hall, Ailsa J.

N1 - This work was supported in part by National Capability funding to Sea Mammal Research Unit, University of St Andrews (Grant No. SMRU 1001) from the UK Natural Environment Research Council. L.M.T. was supported by internal funding from Plymouth University.

PY - 2017/2/27

Y1 - 2017/2/27

N2 - Glucose is an important metabolic fuel and circulating levels are tightly regulated in most mammals, but can drop when body fuel reserves become critically low. Glucose is mobilized rapidly from liver and muscle during stress in response to increased circulating cortisol. Blood glucose levels can thus be of value in conservation as an indicator of nutritional status and may be a useful, rapid assessment marker for acute or chronic stress. However, seals show unusual glucose regulation: circulating levels are high and insulin sensitivity is limited. Accurate blood glucose measurement is therefore vital to enable meaningful health and physiological assessments in captive, wild or rehabilitated seals and to explore its utility as a marker of conservation relevance in these animals. Point-of-care devices are simple, portable, relatively cheap and use less blood compared with traditional sampling approaches, making them useful in conservation-related monitoring. We investigated the accuracy of a hand-held glucometer for ‘instant’ field measurement of blood glucose, compared with blood drawing followed by laboratory testing, in wild grey seals (Halichoerus grypus), a species used as an indicator for Good Environmental Status in European waters. The glucometer showed high precision, but low accuracy, relative to laboratory measurements, and was least accurate at extreme values. It did not provide a reliable alternative to plasma analysis. Poor correlation between methods may be due to suboptimal field conditions, greater and more variable haematocrit, faster erythrocyte settling rate and/or lipaemia in seals. Glucometers must therefore be rigorously tested before use in new species and demographic groups. Sampling, processing and glucose determination methods have major implications for conclusions regarding glucose regulation, and health assessment in seals generally, which is important in species of conservation concern and in development of circulating glucose as a marker of stress or nutritional state for use in management and monitoring.

AB - Glucose is an important metabolic fuel and circulating levels are tightly regulated in most mammals, but can drop when body fuel reserves become critically low. Glucose is mobilized rapidly from liver and muscle during stress in response to increased circulating cortisol. Blood glucose levels can thus be of value in conservation as an indicator of nutritional status and may be a useful, rapid assessment marker for acute or chronic stress. However, seals show unusual glucose regulation: circulating levels are high and insulin sensitivity is limited. Accurate blood glucose measurement is therefore vital to enable meaningful health and physiological assessments in captive, wild or rehabilitated seals and to explore its utility as a marker of conservation relevance in these animals. Point-of-care devices are simple, portable, relatively cheap and use less blood compared with traditional sampling approaches, making them useful in conservation-related monitoring. We investigated the accuracy of a hand-held glucometer for ‘instant’ field measurement of blood glucose, compared with blood drawing followed by laboratory testing, in wild grey seals (Halichoerus grypus), a species used as an indicator for Good Environmental Status in European waters. The glucometer showed high precision, but low accuracy, relative to laboratory measurements, and was least accurate at extreme values. It did not provide a reliable alternative to plasma analysis. Poor correlation between methods may be due to suboptimal field conditions, greater and more variable haematocrit, faster erythrocyte settling rate and/or lipaemia in seals. Glucometers must therefore be rigorously tested before use in new species and demographic groups. Sampling, processing and glucose determination methods have major implications for conclusions regarding glucose regulation, and health assessment in seals generally, which is important in species of conservation concern and in development of circulating glucose as a marker of stress or nutritional state for use in management and monitoring.

KW - Glucose

KW - Glucometer

KW - Phocid

KW - Pinniped

KW - Point-of-care

KW - Validation

U2 - 10.1093/conphys/cox013

DO - 10.1093/conphys/cox013

M3 - Article

VL - 5

JO - Conservation Physiology

JF - Conservation Physiology

SN - 2051-1434

IS - 1

ER -

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ID: 250299060