Skip to content

Research at St Andrews

Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2

Research output: Contribution to journalArticle


Yuanyuan Mei, Peter Thorpe, Athanas Guzha, Annelies Haegeman, Vivian C. Blok, Katrin MacKenzie, Godelieve Gheysen, John T. Jones, Sophie Mantelin

School/Research organisations


Analysis of the genome sequence of the potato cyst nematode, Globodera pallida, has shown that a substantial gene family (approximately 300 sequences) of proteins containing a SPRY domain is present in this species. This is a huge expansion of the gene family as compared to other organisms, including other plant-parasitic nematodes. Some SPRY domain proteins from G. pallida and G. rostochiensis have signal peptides for secretion and are deployed as effectors. One of these SPRYSEC proteins has been shown to suppress host defence responses. We describe further analysis of this gene family in G. pallida. We show that only a minority (10%) of the SPRY domain proteins in this species have a predicted signal peptide for secretion and that the presence of a signal peptide is strongly correlated with the corresponding gene being expressed at the early stages of parasitism. The data suggest that while the gene family is greatly expanded, only a minority of SPRY domain proteins in G. pallida are SPRYSEC candidate effectors. We show that several new SPRYSECs from G. pallida are expressed in the dorsal gland cell and demonstrate that some, but not all, of the SPRYSECs can suppress the hypersensitive response induced by co-expression of the resistance gene Gpa2 and its cognate avirulence factor RBP-1 in Nicotiana benthamiana.



Original languageEnglish
Pages (from-to)409-424
Number of pages16
Issue number4
Early online date25 Feb 2015
Publication statusPublished - 2015

    Research areas

  • Dorsal pharyngeal gland, Gpa2 gene, Hypersensitive response, Parasitism, Plant cell death, SPRYSEC protein

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The transcriptomes of Xiphinema index and Longidorus elongatus suggest independent acquisition of some plant parasitism genes by horizontal gene transfer in early-branching nematodes

    Danchin, E. G. J., Perfus-Barbeoch, L., Rancurel, C., Thorpe, P., Da Rocha, M., Bajew, S., Neilson, R., Guzeeva, E. S., Da Silva, C., Guy, J., Labadie, K., Esmenjaud, D., Helder, J., Jones, J. T. & den Akker, S. E., 23 Oct 2017, In : Genes. 8, 10, 287.

    Research output: Contribution to journalArticle

  2. The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence

    Eves-van den Akker, S., Laetsch, D. R., Thorpe, P., Lilley, C. J., Danchin, E. G. J., Da Rocha, M., Rancurel, C., Holroyd, N. E., Cotton, J. A., Szitenberg, A., Grenier, E., Montarry, J., Mimee, B., Duceppe, M-O., Boyes, I., Marvin, J. M. C., Jones, L. M., Yusup, H. B., Lafond-Lapalme, J., Esquibet, M. & 14 others, Sabeh, M., Rott, M., Overmars, H., Finkers-Tomczak, A., Smant, G., Koutsovoulos, G., Blok, V., Mantelin, S., Cock, P. J. A., Phillips, W., Henrissat, B., Urwin, P. E., Blaxter, M. & Jones, J. T., 10 Jun 2016, In : Genome Biology. 17, 1, p. 1-23 23 p., 124.

    Research output: Contribution to journalArticle

  3. Suppression of plant defences by plant-parasitic nematodes

    Mantelin, S., Thorpe, P. & Jones, J. T., Mar 2015, Plant nematode interactions: a view on compatible interrelationships. Escobar, C. & Fenoll, C. (eds.). Vol. 73. p. 325-337 13 p. (Advances in Botanical Research).

    Research output: Chapter in Book/Report/Conference proceedingChapter

  4. Genomic characterisation of the effector complement of the potato cyst nematode Globodera pallida

    Thorpe, P., Mantelin, S., Cock, P. JA., Blok, V. C., Coke, M. C., Eves-van den Akker, S., Guzeeva, E., Lilley, C. J., Smant, G., Reid, A. J., Wright, K. M., Urwin, P. E. & Jones, J. T., 23 Oct 2014, In : BMC Genomics. 15, 15 p., 923.

    Research output: Contribution to journalArticle

Related by journal

  1. Susceptibility of potato varieties to populations of Globodera pallida selected for increased virulence

    Varypatakis, K., Jones, J. & Blok, V. C., 9 Sep 2019, In : Nematology. Advance Articles , 4 p.

    Research output: Contribution to journalArticle

  2. Characterization of glutathione S-transferases from the pine wood nematode, Bursaphelenchus xylophilus

    Espada, M., Jones, J. T. & Mota, M., 2016, In : Nematology. 18, 6, p. 697-709 13 p.

    Research output: Contribution to journalArticle

  3. Plant-parasitic nematode feeding tubes and plugs: new perspectives on function

    Eves-van den Akker, S., Lilley, C. J., Jones, J. T. & Urwin, P. E., 2015, In : Nematology. 17, 1, p. 1-9 9 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Nematology (Journal)

    John Jones (Member of editorial board)
    2007 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

ID: 208025905