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Optical transfection of mammalian cells - art. no. 61910D

Research output: Chapter in Book/Report/Conference proceedingConference contribution

DOI

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Optical transfection of mammalian cells - art. no. 61910D. / Stevenson, David James; Agate, Ben; Paterson, Lynn; Lake, Tanya; Comrie, Muriel; Brown, Tom; Riches, Andrew; Bryant, Peter; Sibbett, Wilson; Gunn-Moore, Frank; Dholakia, Kishan.

Biophotonics and new therapy frontiers . ed. / R. Grzymala; O. Haeberle. SPIE, 2006. p. 1910-1910 (Proceeding of SPIE - The International Society for Optical Engineering; Vol. 6191).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Harvard

Stevenson, DJ, Agate, B, Paterson, L, Lake, T, Comrie, M, Brown, T, Riches, A, Bryant, P, Sibbett, W, Gunn-Moore, F & Dholakia, K 2006, Optical transfection of mammalian cells - art. no. 61910D. in R Grzymala & O Haeberle (eds), Biophotonics and new therapy frontiers . Proceeding of SPIE - The International Society for Optical Engineering, vol. 6191, SPIE, pp. 1910-1910, Conference on Biophotonics and New Therapy Frontiers, Strasbourg, France, 3/04/06. https://doi.org/10.1117/12.662325

APA

Stevenson, D. J., Agate, B., Paterson, L., Lake, T., Comrie, M., Brown, T., Riches, A., Bryant, P., Sibbett, W., Gunn-Moore, F., & Dholakia, K. (2006). Optical transfection of mammalian cells - art. no. 61910D. In R. Grzymala, & O. Haeberle (Eds.), Biophotonics and new therapy frontiers (pp. 1910-1910). (Proceeding of SPIE - The International Society for Optical Engineering; Vol. 6191). SPIE. https://doi.org/10.1117/12.662325

Vancouver

Stevenson DJ, Agate B, Paterson L, Lake T, Comrie M, Brown T et al. Optical transfection of mammalian cells - art. no. 61910D. In Grzymala R, Haeberle O, editors, Biophotonics and new therapy frontiers . SPIE. 2006. p. 1910-1910. (Proceeding of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.662325

Author

Stevenson, David James ; Agate, Ben ; Paterson, Lynn ; Lake, Tanya ; Comrie, Muriel ; Brown, Tom ; Riches, Andrew ; Bryant, Peter ; Sibbett, Wilson ; Gunn-Moore, Frank ; Dholakia, Kishan. / Optical transfection of mammalian cells - art. no. 61910D. Biophotonics and new therapy frontiers . editor / R. Grzymala ; O. Haeberle. SPIE, 2006. pp. 1910-1910 (Proceeding of SPIE - The International Society for Optical Engineering).

Bibtex - Download

@inproceedings{ce44d531be5a417ab59fc5326a03e58c,
title = "Optical transfection of mammalian cells - art. no. 61910D",
abstract = "The introduction of naked DNA or other membrane impermeable substances into a cell (transfection) is a ubiquitous problem in cell biology. This problem is particularly challenging when it is desired to load membrane impermeable substances into specific cells, as most transfection technologies (such as liposomal transfection) are based on treating a global population of cells. The technique of optical transfection, using a focused laser to open a small transient hole in the membrane of a biological cell (photoporation) to load membrane impermeable DNA into it, allows individual cells to be targeted for transfection, while leaving neighbouring cells unaffected. Unlike other techniques used to perform single cell transfection, such as microinjection, optical transfection can be performed in an entirely closed system, thereby maintaining sterility of the sample during treatment. Here, we are investigating the introduction and subsequent expression of foreign DNA into living mammalian cells by laser-assisted photoporation with a femtosecond-pulsed titanium sapphire laser at 800 nm, in cells that are adherent.",
keywords = "optical transfection, optoporation, femtosecond-pulsed laser, Chinese Hamster Ovary cells, green fluorescent protein, viability, plasmid, GENE DELIVERY, INFRARED-LASER, OPTOPORATION",
author = "Stevenson, {David James} and Ben Agate and Lynn Paterson and Tanya Lake and Muriel Comrie and Tom Brown and Andrew Riches and Peter Bryant and Wilson Sibbett and Frank Gunn-Moore and Kishan Dholakia",
year = "2006",
doi = "10.1117/12.662325",
language = "English",
isbn = "0-8194-6247-0",
series = "Proceeding of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
pages = "1910--1910",
editor = "R. Grzymala and O. Haeberle",
booktitle = "Biophotonics and new therapy frontiers",
address = "United States",
note = "Conference on Biophotonics and New Therapy Frontiers ; Conference date: 03-04-2006 Through 05-04-2006",

}

RIS (suitable for import to EndNote) - Download

TY - GEN

T1 - Optical transfection of mammalian cells - art. no. 61910D

AU - Stevenson, David James

AU - Agate, Ben

AU - Paterson, Lynn

AU - Lake, Tanya

AU - Comrie, Muriel

AU - Brown, Tom

AU - Riches, Andrew

AU - Bryant, Peter

AU - Sibbett, Wilson

AU - Gunn-Moore, Frank

AU - Dholakia, Kishan

PY - 2006

Y1 - 2006

N2 - The introduction of naked DNA or other membrane impermeable substances into a cell (transfection) is a ubiquitous problem in cell biology. This problem is particularly challenging when it is desired to load membrane impermeable substances into specific cells, as most transfection technologies (such as liposomal transfection) are based on treating a global population of cells. The technique of optical transfection, using a focused laser to open a small transient hole in the membrane of a biological cell (photoporation) to load membrane impermeable DNA into it, allows individual cells to be targeted for transfection, while leaving neighbouring cells unaffected. Unlike other techniques used to perform single cell transfection, such as microinjection, optical transfection can be performed in an entirely closed system, thereby maintaining sterility of the sample during treatment. Here, we are investigating the introduction and subsequent expression of foreign DNA into living mammalian cells by laser-assisted photoporation with a femtosecond-pulsed titanium sapphire laser at 800 nm, in cells that are adherent.

AB - The introduction of naked DNA or other membrane impermeable substances into a cell (transfection) is a ubiquitous problem in cell biology. This problem is particularly challenging when it is desired to load membrane impermeable substances into specific cells, as most transfection technologies (such as liposomal transfection) are based on treating a global population of cells. The technique of optical transfection, using a focused laser to open a small transient hole in the membrane of a biological cell (photoporation) to load membrane impermeable DNA into it, allows individual cells to be targeted for transfection, while leaving neighbouring cells unaffected. Unlike other techniques used to perform single cell transfection, such as microinjection, optical transfection can be performed in an entirely closed system, thereby maintaining sterility of the sample during treatment. Here, we are investigating the introduction and subsequent expression of foreign DNA into living mammalian cells by laser-assisted photoporation with a femtosecond-pulsed titanium sapphire laser at 800 nm, in cells that are adherent.

KW - optical transfection

KW - optoporation

KW - femtosecond-pulsed laser

KW - Chinese Hamster Ovary cells

KW - green fluorescent protein

KW - viability

KW - plasmid

KW - GENE DELIVERY

KW - INFRARED-LASER

KW - OPTOPORATION

U2 - 10.1117/12.662325

DO - 10.1117/12.662325

M3 - Conference contribution

SN - 0-8194-6247-0

T3 - Proceeding of SPIE - The International Society for Optical Engineering

SP - 1910

EP - 1910

BT - Biophotonics and new therapy frontiers

A2 - Grzymala, R.

A2 - Haeberle, O.

PB - SPIE

T2 - Conference on Biophotonics and New Therapy Frontiers

Y2 - 3 April 2006 through 5 April 2006

ER -

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    Research output: Chapter in Book/Report/Conference proceedingConference contribution

ID: 5835694

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