Skip to content

Research at St Andrews

Optimisation of wavelength modulated Raman spectroscopy: towards high throughput cell screening

Research output: Contribution to journalArticle

Author(s)

Bavishna B. Praveen, Michael Mazilu, Robert F. Marchington, C. Simon Herrington, Andrew Riches, Kishan Dholakia

School/Research organisations

Abstract

In the field of biomedicine, Raman spectroscopy is a powerful technique to discriminate between normal and cancerous cells. However the strong background signal from the sample and the instrumentation affects the efficiency of this discrimination technique. Wavelength Modulated Raman spectroscopy (WMRS) may suppress the background from the Raman spectra. In this study we demonstrate a systematic approach for optimizing the various parameters of WMRS to achieve a reduction in the acquisition time for potential applications such as higher throughput cell screening. The Signal to Noise Ratio (SNR) of the Raman bands depends on the modulation amplitude, time constant and total acquisition time. It was observed that the sampling rate does not influence the signal to noise ratio of the Raman bands if three or more wavelengths are sampled. With these optimised WMRS parameters, we increased the throughput in the binary classification of normal human urothelial cells and bladder cancer cells by reducing the total acquisition time to 6 s which is significantly lower in comparison to previous acquisition times required for the discrimination between similar cell types.

Close

Details

Original languageEnglish
Article numbere67211
Number of pages5
JournalPLoS One
Volume8
Issue number6
DOIs
Publication statusPublished - 25 Jun 2013

    Research areas

  • Absorption spectroscopy, Wavelength Modulated Raman spectroscopy (WMRS), Cell screening, Signal to Noise Ratio (SNR), Urothelial cells , Bladder cancer cells

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Nonredundant Raman imaging using optical eigenmodes

    Kosmeier, S., Zolotovskaya, S., De Luca, A. C., Riches, A. C., Herrington, C. S., Dholakia, K. & Mazilu, M., 20 Oct 2014, In : Optica. 1, 4, p. 257-263 7 p.

    Research output: Contribution to journalArticle

  2. Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis

    Balagopal, B., Ashok, P. C., Mazilu, M., Riches, A. C., Herrington, C. S. & Dholakia, K., 9 Jul 2012, In : Journal of Biomedical Optics. 17, 7, 6 p., 077006.

    Research output: Contribution to journalArticle

  3. Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples

    Canetta, E., Mazilu, M., De Luca, A. C., Carruthers, A. E., Dholakia, K., Neilson, S., Sargeant, H., Briscoe, T., Herrington, C. S. & Riches, A. C., Mar 2011, In : Journal of Biomedical Optics. 16, 3, 7 p., 037002.

    Research output: Contribution to journalArticle

  4. Optimal algorithm for fluorescence suppression of modulated Raman spectroscopy

    Mazilu, M., De Luca, A. C., Riches, A., Herrington, C. S. & Dholakia, K., 24 May 2010, In : Optics Express. 18, 11, p. 11382-11395 14 p.

    Research output: Contribution to journalArticle

  5. Online Fluorescence Suppression in Modulated Raman Spectroscopy

    De Luca, A. C., Mazilu, M., Riches, A. C., Herrington, C. S. & Dholakia, K., 15 Jan 2010, In : Analytical Chemistry. 82, 2, p. 738-745 8 p.

    Research output: Contribution to journalArticle

Related by journal

  1. PLoS ONE (Journal)

    Joanna Louise Kershaw (Reviewer)
    18 Mar 2019

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. PLoS ONE (Journal)

    Catharine Penelope Cross (Member of editorial board)
    1 Sep 2018 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  3. PLoS ONE (Journal)

    Will Cresswell (Reviewer)
    23 May 2018

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  4. PLoS ONE (Journal)

    Barbara Dritschel (Member of editorial board)
    May 2018 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  5. PLoS ONE (Journal)

    Simon Young (Reviewer)
    7 Jun 201719 Jun 2017

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. Drivers of the opioid crisis: An appraisal of financial conflicts of interest in clinical practice guideline panels at the peak of opioid prescribing

    Spithoff, S., Leece, P., Sullivan, F., Persaud, N., Belesiotis, P. & Steiner, L., 24 Jan 2020, In : PLoS ONE. 15, 1, 15 p., e0227045.

    Research output: Contribution to journalArticle

  2. Satellite derived offshore migratory movements of southern right whales (Eubalaena australis) from Australian and New Zealand wintering grounds

    Mackay, A. I., Bailleul, F., Carroll, E. L., Andrews-Goff, V., Baker, C. S., Bannister, J., Boren, L., Carlyon, K., Donnelly, D. M., Double, M., Goldsworthy, S. D., Harcourt, R., Holman, D., Lowther, A., Parra, G. J. & Childerhouse, S. J., 7 May 2020, In : PLoS ONE. 15, 5, e0231577.

    Research output: Contribution to journalArticle

  3. The shield of self-compassion: a buffer against disordered eating risk from physical appearance perfectionism

    Bergunde, L. & Dritschel, B., 13 Jan 2020, In : PLoS ONE. 15, 1, 17 p., e0227564.

    Research output: Contribution to journalArticle

  4. Under his thumb the effect of president Donald Trump’s Twitter messages on the US stock market

    Brans, H. & Scholtens, B., 11 Mar 2020, In : PLoS ONE. 15, 3, 11 p., e0229931.

    Research output: Contribution to journalArticle

ID: 103066397

Top