Skip to content

Research at St Andrews

Ovarian hormones induce de novo DNA methyltransferase expression in the Siberian hamster suprachiasmatic nucleus

Research output: Contribution to journalArticle

DOI

Open Access Status

  • Embargoed (until 1/01/50)

Author(s)

C. S. Coyle, F. Caso, E Tolla, P. J. Barrett, K. G. Onishi, J. A. Tello, T. J. Stevenson

School/Research organisations

Abstract

Experiments investigated neuroanatomically localized changes in de novo DNA methyltransferase expression in the female Siberian hamster (Phodopus sungorus). The objectives were to identify the neuroendocrine substrates that exhibit rhythmic Dnmt3a and Dnmt3b expression across the oestrous cycle and examine the role of ovarian steroids. Hypothalamic Dnmt3a expression was observed to significantly increase during the transition from proestrous to oestrous. A single bolus injection of diethylstilbestrol (DES) and progesterone was sufficient to increase Dnmt3a cell numbers and Dnmt3b immunoreactive intensity in the suprachiasmatic nucleus (SCN). In vitro analyses using an embryonic rodent cell line revealed that DES was sufficient to induce Dnmt3b expression. Upregulating DNA methylation in vitro reduced expression of vasoactive intestinal polypeptide, Vip, and the circadian clock gene, Bmal1. Together, these data indicate that ovarian steroids drive de novo DNA methyltransferase expression in the mammalian suprachiasmatic nucleus and increased methylation may regulate genes involved in the circadian timing of oestrous: Vip and Bmal1. Overall, epigenetically mediated neuroendocrine reproductive events may reflect an evolutionarily ancient process involved in the timing of female fertility.
Close

Details

Original languageEnglish
JournalJournal of Neuroendocrinology
VolumeIn press
DOIs
Publication statusAccepted/In press - 3 Dec 2019

    Research areas

  • Circadian, Neuroendocrine, Oestrogen, Rhythmic epigenetics

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Medial amygdala Kiss1 neurons mediate female pheromone stimulation of luteinizing hormone in male mice

    Aggarwal, S., Tang, C., Sing, K., Kim, H. W., Millar, R. P. & Tello, J. A., Apr 2019, In : Neuroendocrinology. 108, 3, p. 172–189 18 p.

    Research output: Contribution to journalArticle

  2. Photostimulation for in vitro optogenetics with high power blue organic light-emitting diodes

    Morton, A., Murawski, C., Deng, Y., Keum, C., Miles, G. B., Tello, J. A. & Gather, M. C., Mar 2019, In : Advanced Biosystems. 3, 3, 8 p., 1800290.

    Research output: Contribution to journalArticle

  3. Light-sheet microscopy with attenuation-compensated propagation-invariant beams

    Nylk, J., McCluskey, K. A., Preciado, M., Mazilu, M., Yang, Z., Gunn-Moore, F. J., Aggarwal, S., Tello, J. A., Ferrier, D. E. K. & Dholakia, K., 6 Apr 2018, In : Science Advances. 4, 4, 14 p., eaar4817.

    Research output: Contribution to journalArticle

Related by journal

  1. Social creatures: model animal systems for studying the neuroendocrine mechanisms of social behaviour

    Robinson, K. J., Bosch, O. J., Levkowitz, G., Busch, K. E., Jarman, A. & Ludwig, M., 28 Nov 2019, In : Journal of Neuroendocrinology. 31, 12, p. 1-12 12 p., e12807.

    Research output: Contribution to journalReview article

  2. A role for nonapeptides and dopamine in nest-building behaviour

    Hall, Z. J., Healy, S. D. & Meddle, S., Feb 2015, In : Journal of Neuroendocrinology. 25, p. 158-165 8 p.

    Research output: Contribution to journalArticle

ID: 263999444

Top